FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324282
Sequences flagged as poor quality0
Sequence length19-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG62450.4715762956832457No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAAATTGTTGCTGCCTGCATATGAGCTGCAG56590.427325901884946No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCC49250.37189964071096643No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48130.36344222756180333No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAAATTGTTGCTGCCTGCATATGAGCTGCAGTAATAATCAG45170.3410904928104437No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG43470.3282533478518926No Hit
CTCCTGCACTGGGACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACTCAGGCAAAGC41930.3166244047717933No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC40150.30318315887401626No Hit
GATTATTACTGCAGCTCATATGCAGGCAGCAACAATTTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG36290.2740352885563649No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT34900.26353903473731427No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAACTTCAGGAAGATCTGGTTCTTGGACGTGTCCATTGA34430.25998994171936185No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC34200.25825315151908734No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG33950.25636533608400625No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGGACCAGCAGTGACGTT31600.23861987099424442No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31200.23559936629811476No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30430.22978489475806516No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30220.22819912979259704No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG29130.2199682544956437No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC28140.21249250537272274No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28050.21181289181609356No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27940.21098225302465787No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG27560.2081127735633347No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT26980.20373304175394666No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26450.19973087303157486No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC25070.18931013182992745No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24970.18855500565589503No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24800.18727129116003993No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC23990.18115476915037734No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG23870.18024861774153844No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23650.17858734015866712No Hit
GTCAGTCACCATCTCCTGCACTGGGACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA23490.17737913828021523No Hit
GGCCTGGGCTCTGCTCCTCTCCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC23150.174811709288505No Hit
GGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGCAACAATTTATTCGGCGGAGGGACCAAGCTGACCG22970.17345248217524664No Hit
TCTCCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGG22510.16997890177469752No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22080.16673185922635814No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAAATTGTTGCTGCCTGCATATGAGCTGCAGTAATAATCAGC22040.16642980875674515No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACGCGGAGGGAGG21460.16205007694735715No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACTCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCACTAAG21210.16016226151227608No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21180.15993572366006636No Hit
ACCATGGCCTGGGCTCTGCTCCTCTCCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGAC21010.15865200916421124No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCTCCACCCTCCTC20980.15842547131200152No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG20860.15751931990316262No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT20810.15714175681614642No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAAATTGT20500.15480086567664592No Hit
CCCCTGAGGGCCGCTTAGTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGAGTGCTGTTGGTACCAGGAGA20360.15374368903300054No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19600.14800473011035414No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAAATTGTTGCTGC18990.1433984604487564No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18950.1430964099791434No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGCTTAGTGACCTCA18680.1410575693092559No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18480.13954731696119105No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGCT18230.13765950152610998No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG17900.13516758515180302No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGGACCAGCAG17840.13471450944738356No Hit
GTGTATTACTGTGCGAGGGATATTGGATTCTATGCTTTTGATATGTGGGGCCAGGGGACAGTGGTCACCGTCTCT17800.1344124589777706No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA17360.13108990381202795No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGGACCAGCAGTGACGT17310.13071234072501173No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17300.1306368281076085No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCG17160.1295796514639631No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17110.1292020883769469No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG16860.12731427294186587No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCC16760.12655914676783345No Hit
GTACCAACAGCACTCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCACTAAGCGGCCCTCAGGGGTCCCTGA16700.12610607106341398No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCT16500.12459581871534914No Hit
GCTTGGTCCCTCCGCCGAATAAATTGTTGCTGCCTGCATATGAGCTGCAGTAATAATCAGCCTCATCCTCAGC16380.12368966730651025No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGCAACAA16380.12368966730651025No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG16180.12217941495844539No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16150.12195287710623569No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16060.1212732635496065No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16030.12104672569739677No Hit
GGCAAAGCCCCCAAACTCATGATTTATGAGGTCACTAAGCGGCCCTCAGG15890.1199895490537514No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAACTTCAGGAAGATCTGGTTCTTGGACGTGTCCAT15800.11930993549712221No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15540.11734660744463792No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGGACCAGCAGTGACGTTGGTGG15260.11523225415734716No Hit
CCCCAGGGAAGGGCCTGGAGTGGCTTGCCGATATCTATCTCAGTGGGAGC15230.11500571630513742No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15110.11409956489629852No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14900.11251379993083045No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14730.11123008543497534No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14720.11115457281757209No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14690.11092803496536235No Hit
CATATGCAGGCAGCAACAATTTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCC14530.10971983308691048No Hit
GCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACTCA14390.1086626564432651No Hit
GTTATAACCACCAACGTCACTGCTGGTCCCAGTGCAGGAGATGGTGACTG14290.10790753026923268No Hit
CTATGTCTCCTGGTACCAACAGCACTCAGGCAAAGCCCCCAAACTCATGA14260.10768099241702297No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14120.10662381577337757No Hit
GCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC14020.10586868959934516No Hit
CCTGAGGGCCGCTTAGTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGAGTGCTGTTGGTACCAGGAGACA14020.10586868959934516No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACT13910.1050380508079095No Hit
CCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGCAACAATT13890.104887025573103No Hit
CCTCTCCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCC13810.10428292463387706No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT13680.10330126060763493No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAG13470.10171549564216686No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC13420.10133793255515064No Hit
CGTCACCACCATGGCCTGGGCTCTGCTCCTCTCCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC13420.10133793255515064No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13410.10126241993774739No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13400.10118690732034416No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG13350.10080934423332795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)