FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324282
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT58820.4441652155658689No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAAATT50940.3846612730521143No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG49010.37008733789328857No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCT46970.3546827639430272No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAAATTGTTGCTGCCTGCATATGAGCTGCAGTAATAATCA43260.3266675828864245No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42700.3224388763118429No Hit
CTCCTGCACTGGGACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACTCAGGCAAAGC42540.3212306744333911No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37740.28498461807983494No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC36590.2763006670784622No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC36440.27516797781741353No Hit
GATTATTACTGCAGCTCATATGCAGGCAGCAACAATTTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCA35640.26912696842515416No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32960.24888958696108535No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAACTTCAGGAAGATCTGGTTCTTGGACGTGTCCATT32640.2464731832041816No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGGACCAGCAGTGACGTT32090.24231998924700326No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31190.23552385368071152No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG31070.23461770227187262No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA30370.22933181905364566No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA29540.2230642718091766No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28080.21203942966830325No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG26870.202902402962511No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26470.19988189826638134No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26390.1992777973271554No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGCAACAATTTATTCGGCG25820.1949735781351706No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG25290.19097140941279878No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG24410.18432629908131348No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA24350.18387322337689405No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG23610.17828528968905413No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG23440.17700157519319903No Hit
GTCAGTCACCATCTCCTGCACTGGGACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA23360.17639747425397312No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT22690.17133812888795588No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT22620.1708095405661332No Hit
GGCCTGGGCTCTGCTCCTCTCCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC22610.17073402794872994No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACTCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCACTAA22190.1675624980177938No Hit
GGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGCAACAATTTATTCGGCGGAGGGACCAAGCTGACCGT21820.16476853117387386No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCTCCACCCTCCTC21600.16310725359100253No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21600.16310725359100253No Hit
TCTCCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT21500.1623521274169701No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG21340.16114392553851822No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGA21130.15955816057305017No Hit
CCCCTGAGGGCCGCTTAGTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGAGTGCTGTTGGTACCAGGA20800.15706624419874318No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20790.15699073158133992No Hit
ACCATGGCCTGGGCTCTGCTCCTCTCCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT20610.15563150446808158No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT20080.15162933574570975No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC19850.14989254554543519No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAAATTGTTGCTGCCTGCATATGAGCTGCAGTAATAATCAGC19140.14453114970980502No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT18920.1428698721269337No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18740.14151064501367533No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACGCGGAGGGAGGCTGAGTCAGGGCAGACTGGGCCCAG18500.13969834219599753No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18490.1396228295785943No Hit
GTGTATTACTGTGCGAGGGATATTGGATTCTATGCTTTTGATATGTGGGG18400.1389432160219651No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGGACCA18290.13811257723052944No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC18160.1371309132042873No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC18130.13690437535207758No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGGACCAGCAGT17830.13463899682998035No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG17730.1338838706559479No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAAATTGTTGCTGC17680.13350630756893167No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT17530.13237361830788305No Hit
GTACCAACAGCACTCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCACTAAGCGGCCCTCAGGGGTCCCT16740.12640812153302694No Hit
GCTTGGTCCCTCCGCCGAATAAATTGTTGCTGCCTGCATATGAGCTGCAGTAATAATCAGCCTCATCCTCAGC16390.12376517992391349No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA16370.123614154689107No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16200.12233044019325189No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG16010.12089570046259031No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCA15910.12014057428855789No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACT15870.1198385238189449No Hit
CCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGC15600.11779968314905738No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA15580.11764865791425089No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAG15220.1149302036877342No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAA15130.11425059013110501No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15000.11326892610486286No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14970.11304238825265314No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14930.11274033778304017No Hit
GGCAAAGCCCCCAAACTCATGATTTATGAGGTCACTAAGCGGCCCTCAGG14830.11198521160900773No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTAT14780.11160764852199154No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14700.11100354758276561No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAG14400.10873816906066835No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT14220.10737894194740999No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCA14070.10624625268636137No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTG13950.10534010127752247No Hit
CTATGTCTCCTGGTACCAACAGCACTCAGGCAAAGCCCCCAAACTCATGA13940.10526458866011923No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAACTTCAGGAAGATCTGGTTCTTGGACGTGTCCATT13930.10518907604271598No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG13680.10330126060763493No Hit
CTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTG13680.10330126060763493No Hit
CAACAGCACTCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCACTAAGCGGCCCTCAGGGGTCCCT13460.10163998302476361No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCC13440.10148895778995712No Hit
CCCCAGGGAAGGGCCTGGAGTGGCTTGCCGATATCTATCTCAGTGGGAGCACCTACTACAACCCGTCCCTCAAGA13370.10096036946813443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)