FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164238
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA64940.557789730278517No Hit
GAGTATTACTACCTGGTTGGCCTGGTATCAGCAGAGACCACGGGAAGCCCCTAAGCTCCTGATCTATAAGGCG47110.4046423497601006No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT40290.3460632619790799No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36450.3130803151932852No Hit
ACAATATACTGATTATCCGAGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA29580.25407176195932446No Hit
GTATATTGTTGGCAATAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCT28260.24273387400170757No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT27520.23637778529819503No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26880.2308806275005626No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25950.22289257007587795No Hit
CTCCAGGCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAGTAACTAC25830.22186185298882188No Hit
GTTTATTACTGTGCGAGAGATTGGGGCCGCTACTGGGGCCAGGGAACCCT25750.2211747082641178No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT24320.20889199631003286No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23910.20537037959592455No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23760.20408198323710441No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23390.2009039388853482No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23190.19918607707358804No Hit
CCTTTAGTAGTTACTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGC23010.19764000144300392No Hit
GGCCTGGAGTGGGTGGCCAGCATAAAGCAAGATGGAAGTGAGAAATACTA22800.1958362465406558No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG22380.1922287367359595No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC21540.18501371712656692No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21110.18132031423128261No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21000.18037549023481453No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG20540.17642440806776621No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20450.17565137025247415No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20360.1748783324371821No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20010.17187207426660184No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTATTGCCAACAATATACTGATTA19990.17170028808542584No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC19950.17135671572307382No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA19930.1711849295418978No Hit
GATCAGCACTGAGCGCAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAG19810.1701542124548417No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19350.16620313028779338No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18850.16190847575839304No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTACTACCTGGTTGGCCTGGTA18780.161307224124277No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18770.161221331033689No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTACTACCTGG18690.16053418630898492No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18440.15838685904428476No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18240.15666899723252461No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC18210.1564113179607606No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT17970.15434988378664843No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC17920.1539204183337084No Hit
GTATTACTACCTGGTTGGCCTGGTATCAGCAGAGACCACGGGAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTA17870.15349095288076836No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17260.14825147435489994No Hit
CTCCTGTGAAGCCTCTGGATTCACCTTTAGTAGTTACTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGC16940.14550289545608372No Hit
GAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGCTGTCCAGTGTGAGGTGCAGCTGG16680.14326967510079555No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT16510.14180949256079942No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC16230.13940448602433522No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG16210.13923269984315922No Hit
GCATAAAGCAAGATGGAAGTGAGAAATACTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG16080.13811608966551514No Hit
GTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA16080.13811608966551514No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16050.1378584103937511No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACA16020.1376007311219871No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG16000.13742894494081107No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATG15930.13682769330669503No Hit
GTTTATTACTGTGCGAGAGATTGGGGCCGCTACTGGGGCCAGGGAACCCTGGTCACCGTCTCTTCAGCATCCCCG15820.13588286931022694No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15810.13579697621963893No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG15800.13571108312905095No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15680.13468036604199485No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15340.1317600009620026No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCGCAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTT15250.13098696314671054No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCGGCCCCAATCTCTCG15220.13072928387494653No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15110.12978445987847845No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14900.12798070497613032No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14710.12634873625495818No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCGCAGAGGACTCACCATGGAATTGGGGCTGAG14600.1254039122584901No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG14570.12514623298672609No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTACTACCTGGTTGGCCTGGTATCAGCAGAGA14410.12377194353731798No Hit
CCCTGAGACTCTCCTGTGAAGCCTCTGGATTCACCTTTAGTAGTTACTGG14360.12334247808437793No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14290.12274122645026188No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14270.12256944026908588No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG14270.12256944026908588No Hit
GGGTTCCCTGGCCCCAGTAGCGGCCCCAATCTCTCGCACAGTAATAAACAGCCGTGTCCTCGGCTCTCAGGCTGT14260.12248354717849788No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14200.12196818863496983No Hit
GGTATCAGCAGAGACCACGGGAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTACTTTACAAATTGGGGTCCCA14150.12153872318202978No Hit
CTCCAGAGACAACGCCAAGATCTCATTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTTTATTA13890.11930550282674161No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC13760.11818889264909752No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13280.1140660243008732No Hit
CAATATACTGATTATCCGAGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA12910.11088797994911694No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCAGGGTTCCC12890.11071619376794091No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12820.11011494213382486No Hit
CTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGATCTCATTGTATCTGCAAATGAA12770.10968547668088484No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCAATTTGTAAAGTAGACGCCTTATAGA12740.10942779740912081No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA12730.10934190431853281No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT12690.10899833195618079No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12670.10882654577500477No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12600.10822529414088872No Hit
GAGAAATACTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGATCTCATTGTATC12530.10762404250677267No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT12310.10573439451383652No Hit
CCCCTAAGCTCCTGATCTATAAGGCGTCTACTTTACAAATTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATC12250.10521903597030847No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACATCCAGATGACCCAGTCTCCTTCCACC12230.10504724978913248No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12070.10367296033972435No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT12000.1030717087056083No Hit
GTCCAGTGTGAGGTGCAGCTGGTGGAATCTGGGGGAGGCTTGGTCCAGTCGGGGGGGTCCCTGAGACTCTCCTG11820.10152563307502419No Hit
GTGTGAGGTGCAGCTGGTGGAATCTGGGGGAGGCTTGGTCCAGTCGGGGGGGTCCCTGAGACTCTCCTGTGAAGC11780.10118206071267216No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11730.10075259525973211No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGGGCTTCTTCCCCCAGGAGCCACTCAG11680.10032312980679209No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11670.10023723671620406No Hit
CCATGGAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGCTGTCCAGTGTGAGGTGCAGCTGG11650.10006545053502806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)