FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164238
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAATGAGATCTTGGCGTTGTCTCTGGA53540.4598716070081891No Hit
GAGTATTACTACCTGGTTGGCCTGGTATCAGCAGAGACCACGGGAAGCCCCTAAGCTCCTGATCTATAAGGCGT43550.37406440951077014No Hit
GTTTATTACTGTGCGAGAGATTGGGGCCGCTACTGGGGCCAGGGAACCCTGGTCACCGTCTCTTCAGCATCCCC39330.3378175252826312No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39080.335670198017931No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA35090.30139885487331625No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34170.2934966905392196No Hit
GTATATTGTTGGCAATAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCA27810.23886868492524724No Hit
ACAATATACTGATTATCCGAGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT27430.23560474748290297No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26110.22426685952528608No Hit
CTCCAGGCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAGTAACTACTAAAGGTGAATCCAGAGGCTTCACA24320.20889199631003286No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC24050.20657288286415665No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT23770.20416787632769245No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23060.19806946689594393No Hit
CCTTTAGTAGTTACTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGC22970.1972964290806519No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCA22290.1914556989206674No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21660.186044434213623No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC21470.18441246549245086No Hit
GGCCTGGAGTGGGTGGCCAGCATAAAGCAAGATGGAAGTGAGAAATACTA21390.1837253207677468No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC21350.18338174840539478No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG20760.17831405606070236No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20570.17668208733953022No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20490.17599494261482618No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19720.16938117463954966No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19530.1677492059183775No Hit
GATCAGCACTGAGCGCAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAA19510.1675774197372015No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC18780.161307224124277No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18470.15864453831604877No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG18430.15830096595369675No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTATTGCCAACAATATACTGATTAT18370.1577856074101687No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA18310.15727024886664068No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA17780.1527179150654763No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17690.15194487725018424No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17360.14911040526078No Hit
GTATTACTACCTGGTTGGCCTGGTATCAGCAGAGACCACGGGAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTA17120.14704897108666784No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG16940.14550289545608372No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16920.14533110927490772No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA16850.14472985764079166No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCGCAGAGGACTCACCATGGAATTGGG16840.14464396455020365No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG16560.14223895801373945No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT16280.13983395147727526No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16040.1377725173031631No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16000.13742894494081107No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA15750.1352816176761109No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTACTACCTGGTTGGCCTGGTATCAGCAGA15650.13442268677023084No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCAATTTGTAAAGTAGACGCCTTATA15570.13373554204552676No Hit
CTCCTGTGAAGCCTCTGGATTCACCTTTAGTAGTTACTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGC15550.13356375586435076No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCGCAGAGGACTCACC15340.1317600009620026No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT15200.1305574976937705No Hit
GCATAAAGCAAGATGGAAGTGAGAAATACTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAAC15030.1290973151537744No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGT15010.12892552897259837No Hit
GAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGCTGTCCAGTGTGAGGTGCAGCTGGTGGAAT14950.12841017042907035No Hit
GGGTTCCCTGGCCCCAGTAGCGGCCCCAATCTCTCGCACAGTAATAAACA14920.12815249115730631No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14890.1278948118855423No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACAT14680.12609105698319417No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14580.1252321260773141No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC14430.12394372971849398No Hit
GTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGG14390.12360015735614195No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14340.12317069190320193No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA14320.1229989057220259No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT14210.12205408172555783No Hit
GGTATCAGCAGAGACCACGGGAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTACTTTACAAATTGGGGTCCCAT14140.12145283009144178No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13760.11818889264909752No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCGCAGAGGACTCACCATGGAATTGGGGCTGA13750.11810299955850952No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCGGCCCCAATCTCTC13740.11801710646792152No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT13730.11793121337733349No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG13720.11784532028674549No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13390.11501084829734128No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC13380.11492495520675325No Hit
CTCCAGAGACAACGCCAAGATCTCATTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTTTATTA13210.11346477266675715No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG13160.1130353072138171No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC13080.11234816248911306No Hit
CCCTGAGACTCTCCTGTGAAGCCTCTGGATTCACCTTTAGTAGTTACTGGATGAGCTGGGTCCGCCAGGCTCCAG13040.11200459012676103No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT12980.11148923158323298No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGT12960.11131744540205696No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12920.11097387303970493No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT12770.10968547668088484No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12730.10934190431853281No Hit
CTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGATCTCATTGTATCTGCAAATGAA12380.10633564614795257No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC12370.10624975305736457No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12330.10590618069501252No Hit
GTGTGAGGTGCAGCTGGTGGAATCTGGGGGAGGCTTGGTCCAGTCGGGGGGGTCCCTGAGACTCTCCTGTGAAGC12220.10496135669854445No Hit
GTCCAGTGTGAGGTGCAGCTGGTGGAATCTGGGGGAGGCTTGGTCCAGTCGGGGGGGTCCCTGAGACTCTCCTGT12210.10487546360795645No Hit
CCCCTAAGCTCCTGATCTATAAGGCGTCTACTTTACAAATTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTG12070.10367296033972435No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11890.10212688470914023No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11800.10135384689384816No Hit
GAGAAATACTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGATCTCATTGTATCT11740.10083848835032011No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11650.10006545053502806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)