FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416236
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGACTGCTCATTTGAAGATACA64950.45861000567702No Hit
GTGTATTACTGTGTGAAACAGTATTACGATTTTTGGAGTGGTACGTCCCCCTTCGACCCCTGGGGCCAGGGAACC40800.28808757862390166No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGACTGCTCATTTGAAGATACAGCGTATTCTTGGAATTGTCTC37560.265210035615533No Hit
GAGTAGTAGTTTACCCCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCA37120.26210320878723603No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT35010.24720456195153917No Hit
GTATTACTGTCATCAGAGTAGTAGTTTACCCCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTG33920.2395081045814398No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33760.23837834937115002No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA30740.21705421977692985No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT28510.2013082565335156No Hit
AGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCAT28260.19954301401743776No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT26880.18979887532868817No Hit
GTATTACTGTGTGAAACAGTATTACGATTTTTGGAGTGGTACGTCCCCCTTCGACCCCTGGGGCCAGGGAACC26370.1861977805958894No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAATATGTTTC26250.18535046418817203No Hit
CAGTAATACGTTGCAGCATCTTCAGCTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA24980.17638303220649665No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT24700.17440596058848948No Hit
CTCCTGTTCAGCCTCTGGATTCACCTTCAGTGGCTATGCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGA23820.16819230693189555No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG22800.160990117466298No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22530.15908365554893394No Hit
CTACTACTCTGATGACAGTAATACGTTGCAGCATCTTCAGCTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTC22430.1583775585425028No Hit
GCACTGAAGACAGAAGACTCATCATGGAGTTCTGGCTGAGCTGGGTTCTC22360.15788329063800102No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22140.15632987722385253No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21720.15336426979684176No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG21610.15258756308976754No Hit
GTCTGAGAGCTGAGGACACGGCTGTGTATTACTGTGTGAAACAGTATTACGATTTTTGGAGTGGTACGTCCCCCT21450.1514578078794777No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21260.15011622356725857No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21210.149763175064043No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA20630.14566781242674243No Hit
GTGTAAGCTACTACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGACTT20390.14397317961130773No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19910.1405839139804383No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC19750.13945415877014847No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG19670.13888928116500357No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19420.13712403864892575No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC19050.13451147972513056No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18970.13394660211998563No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCCCAGGCCCGGGATTCCCAGGTGT18880.13331111481419763No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18810.13281684690969583No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCAGGCCCGGGATTCCCAGGTGTTTCCATTCAGTGA18740.13232257900519404No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18690.1319695305019785No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG18560.13105160439361802No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC18510.13069855589040244No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG18450.13027489768654377No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT18340.12949819097946952No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC18310.12928636187754017No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18260.12893331337432462No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT18070.12759172906210547No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAGGGGGACGTAC17910.12646197385181565No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17880.1262501447498863No Hit
GTACGGGAGCTCTGGGAGAGGAGCCCCAGGCCCGGGATTCCCAGGTGTTT17070.12053075899779415No Hit
GATTTCACCCTCACCATCAATAGCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATCAGAGTAGTAGTTTA16890.11925978438621812No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16700.11791820007399896No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT16670.11770637097206962No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG16660.11763576127142653No Hit
TCCCAGTCCTTCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGG16580.11707088366628161No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC16330.11530564115020377No Hit
GGGTTCCCTGGCCCCAGGGGTCGAAGGGGGACGTACCACTCCAAAAATCGTAATACTGTTTCACACAGTAATACA16290.11502320234763133No Hit
GCTCTCAGACTGCTCATTTGAAGATACAGCGTATTCTTGGAATTGTCTCT16030.11318735013091039No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16010.11304613072962416No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15510.1095156456974685No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT15380.10859771958910804No Hit
CCCTGAGACTCTCCTGTTCAGCCTCTGGATTCACCTTCAGTGGCTATGCT15310.10810345168460622No Hit
GTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTTCAGCCTCTGGATTCACCTTCAG15270.10782101288203379No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC15270.10782101288203379No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT15150.10697369647431643No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14910.1052790636588817No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14760.10421991814923502No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC14650.10344321144216076No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT14520.1025252853338003No Hit
GTATTACGATTTTTGGAGTGGTACGTCCCCCTTCGACCCCTGGGGCCAGG14250.10061882341643624No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14240.10054821371579313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10600.047.31181
GTGGTAT2100.045.1806371
TGTTAGA11000.044.99491570
AATGGTA3100.044.7046270
TCTGGAG33150.044.19409670
TCGTATG3300.041.502840
CGTATGC3450.039.7522441
TATCCCG350.001474967538.7125855
GCCGTCT3600.038.2998446
ATGCCGT3600.038.21608444
TATACAC3750.037.9369933
ATCTCGT3250.037.82996737
AATCTCG3250.037.79229736
GCAATCT3300.037.1734234
GTATCAA27350.037.1687131
CTCTTAT1100.036.9659771
CTTATAC3850.036.9659771
ATCTCCG3850.036.95292710
CCGTCTT3850.036.74776547
GGCAATC3350.036.59779733
Produced by FastQC (version 0.11.5)