FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416236
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGACTGCTCATTTGAAGATACAGCGTATTCTTGGAATTGTCTCTGGA58300.4116545547493497No Hit
GTGTATTACTGTGTGAAACAGTATTACGATTTTTGGAGTGGTACGTCCCCCTTCGACCCCTGGGGCCAGGGAACC39550.27926136604351254No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35960.2539124835126349No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34680.24487444183031642No Hit
GAGTAGTAGTTTACCCCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT34160.24120273739687453No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGACTGCTCATTTGAAGATACAGCGTATTCTTGGAATTGTCTCTG34140.2410615179955883No Hit
GTATTACTGTCATCAGAGTAGTAGTTTACCCCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGT31670.22362092193673935No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA30880.21804275558593342No Hit
CTCCTGTTCAGCCTCTGGATTCACCTTCAGTGGCTATGCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGG25670.18125510155087146No Hit
AGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCATCAAGTATGCTTCCCAGTCCTTCTCA25580.18061961424508344No Hit
GTATTACTGTGTGAAACAGTATTACGATTTTTGGAGTGGTACGTCCCCCTTCGACCCCTGGGGCCAGGGAACC25440.17963107843607987No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC24340.1718640113653374No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23970.16925145244154224No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC23880.16861596513575422No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23760.16776864872803685No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT23750.16769803902739372No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAATATGTTTC23130.16332023758752073No Hit
CAGTAATACGTTGCAGCATCTTCAGCTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA22810.1610607271669411No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG22690.16021341075922374No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21800.15392914740198665No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC21550.15216390488590884No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG21430.1513165884781915No Hit
GTCTGAGAGCTGAGGACACGGCTGTGTATTACTGTGTGAAACAGTATTAC21090.14891585865632564No Hit
CTACTACTCTGATGACAGTAATACGTTGCAGCATCTTCAGCTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTC20720.14630329973253045No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20620.14559720272609933No Hit
GCACTGAAGACAGAAGACTCATCATGGAGTTCTGGCTGAGCTGGGTTCTC20140.14220793709522989No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20020.14136062068751254No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA19630.13860684236243112No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCCCAGGCCCGGGATTCCCAGGTGTTTCCATTCAGTGATCAGCACTGAA19550.13804196475728622No Hit
GTGTAAGCTACTACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGACTTTCTCCTTTGGAGTCACAGACTGAAA19410.13705342894828262No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC19300.1362767222412084No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19210.13564123493542038No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG18890.13338172451484073No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18820.13288745661033896No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCAGGCCCGGGATTCCCAGGTGTTTCCATTCAGT18320.1293569715781833No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC17740.12526160894088273No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17540.12384941492802047No Hit
GGGTTCCCTGGCCCCAGGGGTCGAAGGGGGACGTACCACTCCAAAAATCGTAATACTGTTTCACACAGTAATACA17470.12335514702351867No Hit
GTACGGGAGCTCTGGGAGAGGAGCCCCAGGCCCGGGATTCCCAGGTGTTT17150.12109563660293907No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16870.11911856498493188No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC16640.11749454187014029No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT16480.11636478665985048No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAGGGGGACGTAC16400.11579990905470558No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA16080.11354039863412595No Hit
TCCCAGTCCTTCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGG15810.1116339367167619No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG15630.11036296210518587No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT15550.10979808450004096No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG15530.10965686509875473No Hit
GATTTCACCCTCACCATCAATAGCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATCAGAGTAGTAGTTTA15500.10944503599682538No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15220.10746796437881821No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC15160.10704430617495954No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15130.10683247707303022No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC15030.10612638006659908No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT14890.10513784425759548No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG14830.10471418605373681No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14800.10450235695180746No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT14680.10365504054409011No Hit
GTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTTCAGCCTCTGGATTCACCTTCAGTG14610.10316077263958832No Hit
GTATTACGATTTTTGGAGTGGTACGTCCCCCTTCGACCCCTGGGGCCAGG14600.10309016293894521No Hit
GCTCTCAGACTGCTCATTTGAAGATACAGCGTATTCTTGGAATTGTCTCTGGAGATGGTGAATCTGCCCTTCACG14500.10238406593251408No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14420.10181918832736916No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT14410.10174857862672605No Hit
CCCTGAGACTCTCCTGTTCAGCCTCTGGATTCACCTTCAGTGGCTATGCTATGCACTGGGTCCGCCAGGCTCCAG14310.10104248162029492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)