FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289517
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAACAGTATGGTAGCTCACCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTG47590.3690529089573848No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT40380.31314050144356376No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36570.2835945551706569No Hit
GTAATATAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA31210.24202860450850977No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA29680.23016369695009836No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA29210.22651892142561905No Hit
CAGTAATATAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACAGGGAGTTCTTGGCGTTGTCTC28860.22380472688611316No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT27330.21193981932770178No Hit
GTGTATTACTGTCAACAGTATGGTAGCTCACCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA27200.21093168992731387No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT26740.207364462818249No Hit
GTATGGTAGCTCACCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCA26070.2021687189854806No Hit
GCCTTATATTACTGTGCGAGACGGGGGTACTATGGTTCAGGGAGTGATGC25480.19759336247602782No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTCCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA25430.1972056203989556No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24910.193173102797404No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTCCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA24050.18650393907176097No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23670.18355709928601174No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23530.1824714214702094No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG23440.1817734857314793No Hit
ATATTACTGTGCGAGACGGGGGTACTATGGTTCAGGGAGTGATGCCTACT23250.18030006583860467No Hit
CTCCTGCAGGGCCAGTCAGACTATTAGTAGCAGCTACTTAGGCTGGTACC23130.17936948485363124No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC22260.17262277271257379No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21930.17006367500389682No Hit
GCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTC21560.16719438363356204No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21540.1670392868027331No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA21290.16510057641737177No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21230.16463528592488505No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGTAGCAGCTACTTAGGC21140.16393735018615496No Hit
ATATAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACAGGGAGTTCTTGGCGTTGTCTCTGGAGA21060.16331696286283934No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20960.16254147870869481No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20850.1616884461391358No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC20650.16013747783084675No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19980.15494173399807834No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC19940.15463154033642054No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19820.1537009593514471No Hit
CTCCTGTGTAGGCTCTGGATTCACCTTTGATGATTATGGCATGAGCTGGGTCCGCCAAGCTCCAGGGAAGGGG19820.1537009593514471No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19160.14858276393409314No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19090.14803992502619198No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGGAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAACAGTA18980.147186892456633No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCACTGTGAGGTGCAGGTGGTGGAG18730.14524818207127163No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT18550.14385231059381148No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG18540.14377476217839702No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGTAG18350.1423013422855224No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGTACCATAGATGAGGAGCC18160.1408279223926478No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG18090.1402850834847466No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17910.13888921200728646No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC17800.13803617943772745No Hit
ACCATACTGTTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTCC17590.13640766271402394No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT17580.1363301142986095No Hit
GTTATGGAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCC17490.13563217855987938No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17220.13353837134368915No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG16840.13059153155793993No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT16530.12818753068009184No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC16350.1267916592026317No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC16190.1255508845560004No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG16140.12516314247892818No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGTACCATAGA16100.12485294881727034No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC16000.12407746466312582No Hit
GTGTAGGCTCTGGATTCACCTTTGATGATTATGGCATGAGCTGGGTCCGCCAAGCTCCAGGGAAGGGGCTGGAG15850.12291423843190899No Hit
GACTATTAGTAGCAGCTACTTAGGCTGGTACCAGCAGAAACCTGGCCAGG15700.12175101220069219No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCACGTGTTTCCATTCAGTGA15580.12082043121571875No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCACGTGT15580.12082043121571875No Hit
GTCTGAGAGCCGAGGACACGGCCTTATATTACTGTGCGAGACGGGGGTACTATGGTTCAGGGAGTGATGCCTACT15520.12035514072323203No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG15470.11996739864615975No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15340.11895926924577187No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15340.11895926924577187No Hit
CTACCATACTGTTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTCCTGATGGTGAGAGTGAAGTCTGTC15090.11702055886041052No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14960.11601242946002263No Hit
CCTTGGGGCTGGTCGGGGATGCTGCGGAGACGGTGACCTGGGTTCCCTGGCCCCAGTAGTCAAAGTAGGCATCA14900.11554713896753589No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14870.11531449372129254No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA14710.1140737190746613No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14670.11376352541300347No Hit
GTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCACGTGTTT14530.11267784759720113No Hit
GGGCTGGAGTGGGTCTCTGCTATTACTTGGGATGGTGGTAGCACAAGTTA14210.11019629830393862No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14200.11011874988852415No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTG14140.10965345939603743No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT14090.10926571731896516No Hit
GCCCTGAGATTCCCACGTGTTTCCATTCAGTGATCAGCACTGAACACAGA14080.10918816890355071No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13950.1081800395031628No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT13880.10763720059526163No Hit
GACTTCACTCTCACCATCAGGAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAACAGTATGGTAGCTCA13780.10686171644111711No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCG13760.1067066196102882No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA13690.10616378070238701No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC13430.10414752190161121No Hit
CTCCAGCCCCTTCCCTGGAGCTTGGCGGACCCAGCTCATGCCATAATCAT13400.10391487665536786No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13360.10360468299371005No Hit
GATCAGCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGG13350.1035271345782956No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13280.10298429567039441No Hit
CATCTATGGTACATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA13220.1025190051779077No Hit
ACTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTACATCCAGCAGGGCCAC12940.10034764954630299No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)