FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314253
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGATGGTTCGCTCACTTTCGGCGGAGGGACCAAGGTCGAAATCAAACGAACTG91650.6973543145802217No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT56710.43149987102939846No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA52260.3976403325691476No Hit
GTATGATGGTTCGCTCACTTTCGGCGGAGGGACCAAGGTCGAAATCAAACGAACTGTGGCTGCACCATCTGTCT49170.37412887777315323No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48260.36720479238015813No Hit
GTGTATTACTGTCAGCAGTATGATGGTTCGCTCACTTTCGGCGGAGGGACCAAGGTCGAAATCAAACGAACTG40940.3115077538343074No Hit
GTTAGGACCCAGAGGGAACCATGGATACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA39440.3000944262634363No Hit
CCATCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC37170.2828222572061848No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35200.2678327536631075No Hit
GTGTTAGCACTAGGTTCTTTGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGACTCCTCATTTATGCTACA34930.2657783547003507No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT34190.26014777976538767No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32370.24629960897939743No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA29200.22217944337962325No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTTGGATGTAGCATAAATGAGGAGTC28740.21867935625788948No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28370.21586406879040795No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28230.2147988248837933No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28210.21464664718284834No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC28050.2134292255752888No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTA27640.21030958270591735No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC27080.20604860707945882No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26630.20262460880819752No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26350.20049412099496822No Hit
GTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACTAGGTTCTTTGCC26140.1988962551350463No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25340.1928091470972484No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25110.1910591035363815No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGC24430.18588506170425328No Hit
CTCCCAGACTCCTCATTTATGCTACATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTC24370.18542852860141845No Hit
GTCAGAGTGTTAGCACTAGGTTCTTTGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGACTCCTCATTTA23790.18101537527401498No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCA23640.17987404251692787No Hit
GTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCAAAGAACCTAGTG23090.17568915574094182No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTTGGATGTAGCATAAA22770.17325431252582266No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTGACGCCTGG22710.17279777942298782No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT22240.16922160345078155No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCGACCTTGGTCC22230.16914551460030908No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTGACGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG22190.1688411591984192No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC21260.1617648961044791No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21200.16130836300164428No Hit
CAGTTAGGACCCAGAGGGAACCATGGATACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC21180.16115618530069933No Hit
GGATACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATA21040.1600909413940847No Hit
GTGCAGCCACAGTTCGTTTGATTTCGACCTTGGTCCCTCCGCCGAAAGTG20910.15910178633794253No Hit
GTATTATTGTTCCACGGAGGGGCCGGATGTCCACGGTGAGAGACACCGGGACTGGTTCTTCGATCTCTGGGGCCG20700.1575039204780206No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGACTGAGCTGGATTTTC20290.15438427760864917No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19880.15126463473927773No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGATACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA19810.15073201278597043No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACTAGGTTCTTTGCCTGGTACC19740.15019939083266312No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGATGGTTCG19440.1479167253184889No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT19350.14723192566423662No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT18980.1444166381967551No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18930.14403619394439274No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18610.14160135072927357No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC18180.13832953015895721No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17810.1355142426914757No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC17650.1342968210839161No Hit
GAGTTTGGACTGAGCTGGATTTTCCTTGCCGCTATTTTAAAAGGTGTCCAGTGTGAGGGACAACTGATGGAG17450.13277504407446664No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA17370.13216633327068683No Hit
CAATAATACATGGCTGTGTCTTCGCTTTTCAGGCTGTTCACTTGGAGAAACAACGTGTTTTTTGAATCATCTCT17260.13132935591548964No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA17200.1308728228126548No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16650.12668793603666872No Hit
ACATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTC16280.1238726485691872No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16230.12349220431682484No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16090.12242696041021021No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGACTGAGCTGGATTTTCCTTGCCGCTATTTTAAAAGG15460.11763336283044437No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15440.11748118512949941No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGCGACAGTCGGGGG15220.115807230419105No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC15110.11497025306390779No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15030.11436154226012799No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC14900.11337238720398585No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14690.11177452134406389No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG14600.11108972168981163No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14540.11063318858697678No Hit
GCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA14330.10903532272705484No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGTTCCCATCTTGGGGGG14290.10873096732516493No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14230.1082744342223301No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT14130.10751354571760535No Hit
GTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCCTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGA13640.10378519204445416No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA13520.10287212583878445No Hit
GTTGGATGTAGCATAAATGAGGAGTCTGGGAGCCTGGCCAGGTTTCTGCT13480.10256777043689458No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13450.10233950388547715No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13410.10203514848358725No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13360.10165470423122488No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT13350.10157861538075241No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13340.10150252653027994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)