FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314253
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGATGGTTCGCTCACTTTCGGCGGAGGGACCAAGGTCGAAATCAAACGAACTGTG80990.6162435999765646No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54820.41711907829010086No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA50860.38698789350300133No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA48760.37100923490378185No Hit
GTATGATGGTTCGCTCACTTTCGGCGGAGGGACCAAGGTCGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT44600.33935627310723276No Hit
GTGTATTACTGTCAGCAGTATGATGGTTCGCTCACTTTCGGCGGAGGGACCAAGGTCGAAATCAAACGAACTGTG37710.28693105513169836No Hit
GTTAGGACCCAGAGGGAACCATGGATACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA36380.2768112380188594No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT34560.26296306723286916No Hit
CCATCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC34520.2626587118309793No Hit
GTGTTAGCACTAGGTTCTTTGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGACTCCTCATTTATGCTACAT32820.24972360725065873No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32090.24416912116616815No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC31600.24044076749301693No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31560.24013641209112707No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28040.2133531367248163No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC28010.21312487017339887No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACTAGGTTCTTTGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCA27610.2100813161544999No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT27270.20749429523843582No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTTGGATGTAGCATAAATGAGGAGTCTG26860.2043746523690644No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26820.2040702969671745No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC25590.19471136835906025No Hit
GTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACTAGGTTCTTTGCCTG25440.19357003560197314No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA25250.19212434744299614No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA25180.1915917254896888No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25170.19151563663921634No Hit
GTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCAAAGAACCTAGTGCTAACACTCTGACTGGCCCTGCAG24250.18451546239574876No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT24230.18436328469480381No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACTA23860.1815479972273223No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23070.17553697803999688No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA22360.17013466965645124No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCA22130.16838462609558436No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTGACGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGG21940.16693893793660733No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTGACGCCTGGATGAGCTGGGTCCGCCAGGCTCCAG21920.16678676023566238No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21900.16663458253471744No Hit
CTCCCAGACTCCTCATTTATGCTACATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTG21840.1661780494318826No Hit
GGATACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCA21760.16556933862810283No Hit
GTCAGAGTGTTAGCACTAGGTTCTTTGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGACTCCTCATTTAT21600.16435191702054322No Hit
GTGCAGCCACAGTTCGTTTGATTTCGACCTTGGTCCCTCCGCCGAAAGTG21070.1603192079455021No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA20800.15826480898274534No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC20590.1566669431228234No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC20480.15582996576762617No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCGACCTTGGTCC20470.1557538769171537No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGATGGTTC20160.153395122552507No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGATACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA19840.15096027933738787No Hit
CAGTTAGGACCCAGAGGGAACCATGGATACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC19740.15019939083266312No Hit
GTATTATTGTTCCACGGAGGGGCCGGATGTCCACGGTGAGAGACACCGGG19480.14822108072037882No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19390.14753628106612654No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19380.14746019221565407No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGACTGAGCTGGATTTTC18750.1426665946358882No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18480.14061219567313143No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17660.13437290993438858No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAG17600.13391637683155375No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGTCTGGGCACTGTGTGGCC16010.12181824960643042No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA15990.12166607190548548No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15590.11862251788658652No Hit
GAGTTTGGACTGAGCTGGATTTTCCTTGCCGCTATTTTAAAAGGTGTCCAGTGTGAGGGACAACTGATGGAGT15480.11778554053138932No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCA15410.11725291857808201No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT15370.11694856317619211No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGG15360.11687247432571964No Hit
ACATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTC15060.11458980881154542No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14980.11398109800776562No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14920.11352456490493078No Hit
GTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCCTTCCCCTGGAGACAAAGACAGGGTGCCTGGA14760.1123071432973712No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14700.11185061019453636No Hit
CAATAATACATGGCTGTGTCTTCGCTTTTCAGGCTGTTCACTTGGAGAAACAACGTGTTTTTTGAATCATCTCTT14590.11101363283933915No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGACTGAGCTGGA14590.11101363283933915No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14300.1088070561756374No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14040.10682874606335309No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA13990.10644830181099071No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAACGTGGTCGTCGCATGCCTGGTCCAGG13740.1045460805491789No Hit
GTTGGATGTAGCATAAATGAGGAGTCTGGGAGCCTGGCCAGGTTTCTGCT13660.10393736974539909No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA13520.10287212583878445No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13510.10279603698831198No Hit
GCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGACTGA13370.10173079308169737No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG13340.10150252653027994No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTTCAGGGCCAGCTCCTCCGACGGCGGC13250.10081772687602766No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13240.1007416380255552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAA650.0208.7994770
CGATTAA403.3052135E-4113.09972470
TACGTAT508.026745E-490.47977470
AATGGTA4750.079.36822570
CCGTTTA651.8189894E-1276.7936669
CATTTAA1605.9117156E-965.9748470
GGGCCGT12150.065.7809170
GAATGCA850.006580952653.223470
ACCGTTT908.3309715E-1050.2665470
ATGGCAA1602.9571973E-547.1248870
GGTATCA9150.044.364911
GTTGCGG405.5850083E-542.32722524
TAAACGC405.658271E-542.233825
GAGCAAA24400.040.1720370
TAGGCAA4205.456968E-1239.4951470
GACAGTA18400.039.33903570
CATAAAT23600.037.6999170
TATTCTA1650.001951665536.55748470
AAGGACA25450.036.1445170
TCGTTTT400.00285763933.83210821
Produced by FastQC (version 0.11.5)