FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985825
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCAGTGTCCTCAGCCTTTAGGCTGCTGATCTGCAGATATG53520.5428955443410342No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCCCCCTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA34260.3475261836532853No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT26280.2665787538356199No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA25340.25704359292977963No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24740.25095732001115817No Hit
GTATATTACTGTGCGAGAGATCGTATGCGGGCTTTTGATATCTGGGGCCA23590.23929196358380037No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21800.221134582709913No Hit
GCTCCACACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAG21630.2194101387163036No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT19150.1942535439860016No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18170.1843126315522532No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18060.18319681485050593No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT17510.17761773134176959No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCCCCCTACAGTTTTGGCCA17360.17609616311211423No Hit
GTATGGTAGCTCACCCCCCTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA16920.17163289630512515No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16700.16940126290163063No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15870.16098191869753758No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15530.15753303071031877No Hit
CTCCCAGGTGCAGCTGGTCCAATCTGGGTCTGAGTTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGG15460.1568229655364796No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15350.15570714883473233No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15300.1551999594248472No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG15140.15357695331321483No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14950.15164963355565136No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAGCTACTTAGCCTGGTACC14820.15033094108995004No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14740.14951943803413387No Hit
GTGTTGGTGTTGATCCATCCCATCCACTCAAGCCCTTGTCCAGGGGCCTG14390.145969112164938No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA14050.14252022417771917No Hit
GCCTTTAGGCTGCTGATCTGCAGATATGCCGTGCTGACAGAGGTGTCCAAGGAGAGGACAAACCGTCCTGTGAAG14020.14221591053178811No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13980.14181015900388No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13900.1409986559480638No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGATGAGGAGCC13900.1409986559480638No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA13860.1405929044201557No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG13780.1397814013643395No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13700.13896989830852333No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13660.13856414678061524No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13530.13724545431491392No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC13460.13653538914107474No Hit
CAGTAATATACGGCAGTGTCCTCAGCCTTTAGGCTGCTGATCTGCAGATA13290.1348109451474653No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGGTAACACTCTGA13050.13237643598001675No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12940.13126061927826949No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTA12880.13065199198640734No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12880.13065199198640734No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12640.12821748281895873No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAG12520.12700022823523446No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCA12470.12649303882534932No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG12320.12497147059569397No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12250.1242614054218548No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAGCTACTTAGCC12120.12294271295615349No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12040.12213120990033728No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC11780.11949382496893464No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11490.11655212639160094No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC11490.11655212639160094No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC11490.11655212639160094No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG11360.11523343392589962No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGG11340.11503055816194559No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11160.11320467628635913No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11140.1130018005224051No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC10950.11107448076484164No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC10870.11026297770902543No Hit
CTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTATATTA10810.1096543504171633No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGA10750.10904572312530116No Hit
GTCCAAGGAGAGGACAAACCGTCCTGTGAAGCCCTGGGCATACGTTGGGTTCCCAGTGTTGGTGTTGATCCATCC10480.10630690031192147No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG10470.10620546242994446No Hit
GAGTGTTACCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG10430.10579971090203637No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT10310.10458245631831206No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10140.10285801232470265No Hit
GGACAATGGTCACCGTCTCTTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGC10140.10285801232470265No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10100.10245226079679456No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10060.10204650926888648No Hit
GGGCTTCACAGGACGGTTTGTCCTCTCCTTGGACACCTCTGTCAGCACGG10050.10194507138690943No Hit
GTCAGAGTGTTACCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTA10000.10143788197702433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT150.002454799468.2847239
GCATAGA150.002569624767.501956
AAAACCG908.642664E-658.01427570
TAGTCCG550.005032102656.95946570
GTTATTA550.005032102656.95946570
TACGGTA200.00802739350.6238987
ACGGTAG200.00802739350.6238988
AAGTCGT200.00802739350.62389820
TGTTAGA7300.050.06711270
AACTCTA650.00974167248.1964770
AGTAAGA1351.7466809E-646.4114270
CGCGTAG1650.045.06759328
GGTATCA6850.044.8438871
ACGCGTA1600.044.34764527
GACGCGT1600.044.32738526
AGACGCG1600.044.3206425
GCGTAGT1800.041.33295429
GTCGGTT550.001232405940.14997569
CCGTCTT1750.037.5388347
CGGCAAA1401.17540825E-437.29488870
Produced by FastQC (version 0.11.5)