FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985825
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCAGTGTCCTCAGCCTTTAGGCTGCTGATCTGCAGATATGCCGTGCTGACAGAGGTGTCCAAGGA46040.46702000862222No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCCCCCTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA31940.32399259503461564No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24920.2527832018867446No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA24310.24659549108614612No Hit
GTATATTACTGTGCGAGAGATCGTATGCGGGCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCA24170.24517536073846777No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA22530.2285395480942358No Hit
GCTCCACACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAGCAACAGGTGCCCACTCCCAGGTGCA19910.20196282301625543No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA19370.1964851773894961No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18540.1880658331854031No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18530.18796439530342607No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT17800.1805594299191033No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16220.16453224456673346No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCCCCCTACAGTTTTGGCCA16090.16321355210103214No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG15910.16138767022544567No Hit
GTATGGTAGCTCACCCCCCTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT15830.1605761671696295No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC15760.15986610199579032No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15740.15966322623183626No Hit
GTGTTGGTGTTGATCCATCCCATCCACTCAAGCCCTTGTCCAGGGGCCTGTCGCACCCAATTCATAGCATAGTTA15160.1537798290771689No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC15020.1523596987294905No Hit
CTCCCAGGTGCAGCTGGTCCAATCTGGGTCTGAGTTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAG14820.15033094108995004No Hit
GCCTTTAGGCTGCTGATCTGCAGATATGCCGTGCTGACAGAGGTGTCCAAGGAGAGGACAAACCGTCCTGTGAA14280.14485329546319073No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14040.14241878629574214No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14020.14221591053178811No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13990.14191159688585703No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC13990.14191159688585703No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13800.13998427712829356No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13750.13947708771840844No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAG13650.13846270889863818No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG13580.13775264372479903No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13530.13724545431491392No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA13370.1356224482032815No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGATGAGGAGCCTG13280.1347095072654883No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13150.13339081479978698No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT12750.12933329952070602No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC12600.12781173129105064No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCA12600.12781173129105064No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAGCA12390.12568153576953314No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGGTAACACTCT12390.12568153576953314No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA12050.1222326477823143No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCA11950.12121826896254405No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC11810.11979813861486571No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11710.11878375979509548No Hit
CAGTAATATACGGCAGTGTCCTCAGCCTTTAGGCTGCTGATCTGCAGATATGCCGTGCTGACAGAGGTGTCCAAG11700.11868232191311846No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT11550.11716075368346308No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAGCTACTTAGCCTG11450.11614637486369285No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA11070.11229173534856592No Hit
GGGCTTCACAGGACGGTTTGTCCTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAA10960.11117591864681865No Hit
CTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTATATTA10840.10995866406309435No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10790.10945147465320924No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC10760.10914716100727817No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATA10750.10904572312530116No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT10740.10894428524332411No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT10690.108437095833439No Hit
GAGTGTTACCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG10630.10782846854157685No Hit
GTCCAGGGGCCTGTCGCACCCAATTCATAGCATAGTTAGTGAAGGTGTATCCAGAAGCCTTGCAGGAAACCTTCA10610.10762559277762279No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10580.10732127913169172No Hit
GCCTAAAGGCTGAGGACACTGCCGTATATTACTGTGCGAGAGATCGTATGCGGGCTTTTGATATCTGGGGCCAAG10560.10711840336773769No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAGCCCGCATAC10530.10681408972180662No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10520.10671265183982959No Hit
CACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCCTCTCCTTGGACACCTCTGTCA10410.10559683513808231No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA10330.10478533208226612No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10220.10366951538051884No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC10110.10255369867877158No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG10100.10245226079679456No Hit
GGACAATGGTCACCGTCTCTTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGC10050.10194507138690943No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG10020.10164075774097836No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT9990.10133644409504729No Hit
GTCCAAGGAGAGGACAAACCGTCCTGTGAAGCCCTGGGCATACGTTGGGTTCCCAGTGTTGGTGTTGATCCATCC9950.1009306925671392No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG9900.10042350315725408No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9900.10042350315725408No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATTAT351.4969085E-4137.9846270
AGCCGGT559.0378604E-487.8083970
CGATTAA752.875893E-585.8570970
GTAGTTT1152.8849172E-883.9906470
GTCTTTT700.0023538468.9923170
AATGGTA3600.049.1889670
AAGAGTT1000.00965890248.29461770
TATTCTA1406.3028146E-445.99487370
GAGCCAT12400.042.84270
GGTATCA6300.042.2651671
GTATCAA17800.041.847381
CCTACTT1956.457618E-541.2774570
GTAGATA1600.001216223240.24551470
AAACGAA18650.038.84285470
GAGCAAA14550.037.617870
GGATAAT2251.4998534E-435.77379270
TATCAAC20850.035.700452
ATCAACG21300.035.253533
TTTCCAT2751.1828628E-535.12335670
CAACGCA21900.034.135655
Produced by FastQC (version 0.11.5)