FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780541
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT29220.3743557353169148No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCGGGTCCCTCCGCCGAATACGAAAGTGTTCCTACCTGCATATGAGCA25100.321571832869766No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23950.3068384620410715No Hit
GTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGTGTTCTTGGGATTGTCTCTGGA23820.3051729505560887No Hit
GATTATTACTGCTGCTCATATGCAGGTAGGAACACTTTCGTATTCGGCGGAGGGACCCGGCTGACCGTCCTAGGT22290.28557116154052126No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT21870.2801902782813459No Hit
GACCTAGGACGGTCAGCCGGGTCCCTCCGCCGAATACGAAAGTGTTCCTACCTGCATATGAGCAGCAGTAATAAT21610.2768592553113802No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCT20120.2577699313681152No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCA18780.24060235144598424No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG18440.2362463983314137No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17550.22484405047268496No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG17220.22061621362619005No Hit
GTGTTGGTACCAGGAGACAAGGTTAAAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATC15670.20075819207447143No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC15280.19576165761952288No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTTTAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGC15230.19512107627914485No Hit
GGACTGAACAGAGAGAACTCACCATGGAATTTGGGCTGAGCTGGCTTTTT14870.1905088906284231No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14060.18013147291429918No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA14050.18000335664622358No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG13920.17833784516124074No Hit
GTAATAATCAGCCCCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGA13660.17500682219127503No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13630.17462247338704823No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG13590.1741100083147458No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCGGGTCCCTC13420.17193203175746052No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13290.17026652027247768No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13150.16847289251941922No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT13090.1677041949109656No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTTTAACCTTGTCTCCTGGTACCAACACCA12890.16514186954945353No Hit
CTATATTACTGTGCGAAAGATCTGGATTATGATCTTTGGAGGGGGGCTTTTGATATTTGGGGCCAAGGGACAATG12740.16322012552831947No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA12140.15553314944378321No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGG12110.1551488006395564No Hit
CAGTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGTGTTCTTGGGATTGTCTCTG11800.1511771963292127No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTATGCCATGACCTGCGTCCGCCAGGCTCCAGGGAAGGG11580.1483586384315494No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGG11450.14669312694656655No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11430.14643689441041532No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTG11420.14630877814233975No Hit
ATATTACTGTGCGAAAGATCTGGATTATGATCTTTGGAGGGGGGCTTTTGATATTTGGGGCCAAGGGACAATGGT11380.14579631307003732No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC11350.14541196426581052No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT10940.14015919727471074No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10790.13823745325357667No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGT10400.13324091879862815No Hit
GTCTGGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAA10360.13272845372632572No Hit
CCCCTGAGGGCCGCTTACTGCCCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGGA10360.13272845372632572No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10300.13195975611787208No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT10270.13157540731364528No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9930.12721945419907474No Hit
GTTTTAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGC9880.12657887285869673No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA9870.12645075659062113No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC9790.12542582644601627No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCA9750.12491336137371387No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9740.12478524510563826No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9730.12465712883756266No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9730.12465712883756266No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCGGGTCCCTCCGCCGAATAC9370.12004494318684092No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGAT9140.11709826902110204No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT9140.11709826902110204No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC8840.1132547809788339No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG8840.1132547809788339No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8820.1129985484426827No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAG8790.11261419963845588No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC8540.10941129293656579No Hit
ATATAGGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGTGTTCTTGGGATTGTCTCTGGAGAT8480.10864259532811216No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8420.10787389771965855No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACGCAGGTCATGGCATAGTTGCTAAAGGTGAATCCAGAGGCTGCACA8390.10748954891543173No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT8320.1065927350389025No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTG8280.1060802699666001No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAA8260.1058240374304489No Hit
GTATGTGCTACCGCTACCGCTAATAGTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACGCAGGTCAT8260.1058240374304489No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT8210.10518345609007086No Hit
AGCACATACTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAATCCCAAGAACACACTGTATCT8180.10479910728584405No Hit
ACCTAGGACGGTCAGCCGGGTCCCTCCGCCGAATACGAAAGTGTTCCTAC8030.10287736326470998No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGTCTGGGCACTGTGTGGCC7810.10005880536704671No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)