FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004685824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468503
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGACA98780.6726578018567207No Hit
GTATATTACTGTGTGAGACTAATGTGGGTCGGCAGTGGCTGGGACTTTGA55880.38052356719734315No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT38140.25972027295824385No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37610.2561111553738739No Hit
CAGTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGACAGTGAGTTCTTGGCGTTGTCTC35430.24126610568722026No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA29160.19856956369854198No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT27550.18760601782904085No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC27550.18760601782904085No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG27140.18481405894301886No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26550.18079636200947496No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTATTAACTATTGGATGGGTTGGGTCCGCCAGGCTCCAGGGAAGGGG25940.17664247195953975No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT25310.1723523887932132No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT24870.16935614023260423No Hit
GTAATAAACCCCGACATCCTCAGCTTCCACCCTGCTGATTTTCAGTGTGAAATCTGTCCCTGCCCCACTGCCA24660.16792611251049538No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGG24650.16785801595229974No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA23870.16254648441303832No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGCGACAGTCGGGGG23830.16227409818025568No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC23510.16009500831799459No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC23440.15961833241062498No Hit
ACATAAAGCAGGATGGAATTGAGAAATACTATTTGGACTCTGTGGAGGGCCGATTCACCATCTCCAGAGACAACG23100.15730304943197257No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22300.15185532477631983No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA22080.15035720049601534No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22040.1500848142632327No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21910.14919955900668913No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGG21650.14742904849360197No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG21640.14736095193540633No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21280.14490947584036262No Hit
GTGATGGAAACACCTACTTGAGTTGGCTTCACCAGAGGCCAGGCCAGCCTCCAAGACTCCTAATTTATAGGATT21120.14381993090923206No Hit
ATATTACTGTGTGAGACTAATGTGGGTCGGCAGTGGCTGGGACTTTGACTACTGGGGCCAGGGAACCCTGGTCAC21020.14313896532727546No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC20740.141232261697797No Hit
CTATTTGGACTCTGTGGAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTCTCTGCAAATGAA20730.14116416513960134No Hit
GTCCAAATAGTATTTCTCAATTCCATCCTGCTTTATGTTGGCCACCCACT20140.13714646820605747No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20140.13714646820605747No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20100.13687408197327483No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACCCATCCAATAGTTAA19970.13598882671673126No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG19910.1355802473675573No Hit
GAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCACTGTGAGGTGCAACTGGTGGAG19720.13428641276183978No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAG19570.1332649643889049No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCAGGATGGAATTGAGAAATACTA19550.13312877127251357No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC19540.13306067471431793No Hit
GCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTC19090.12999632959551324No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTT19060.12979203992092628No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA18960.12911107433896968No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18850.12836201219881743No Hit
GTTTATTACTGCATGCAATCTACACAAGCTCCCCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA18480.12584243954557806No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18290.12454860493986053No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC18160.12366334968331696No Hit
GTGCAGGTGAAGGTCTCCCCATGGTTCCATGGCTGGGCACAGCCAGGCAGGACACTGGACACGCTGTAGCAGCCA18090.12318667377594734No Hit
CTATTGGATGGGTTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTGGCCAACATAAAGCAGGA17900.12189283917022982No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17450.11882849405142515No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGTTCCCATCTTGGGGGG16980.11562795581622919No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT16870.11487889367607693No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTCTCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTATATTA15920.10840972064748931No Hit
GTCTGGGACCCCAGAGAACCGGTTAGAAATCCTATAAATTAGGAGTCTTGGAGGCTGGCCTGGCCTCTGGTGAAG15820.10772875506553273No Hit
GTATCAACGCAGAGTACGGGAATTAGGACTCCTCAGGTCACCTTCTCACA15820.10772875506553273No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTATTAACTATTGG15710.10697969292538048No Hit
GCTTTATGTTGGCCACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACCCATCCAATAGTTAATAAAGG15710.10697969292538048No Hit
CTCCTCACCTGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACACAGTGA15650.10657111357620652No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15580.10609443766883689No Hit
GTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGATTTCACACTGAAAATCAGCAGGGTGGAAGCTGAGGA15570.10602634111064124No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGG15490.10548156864507596No Hit
GTTCCATGGCTGGGCACAGCCAGGCAGGACACTGGACACGCTGTAGCAGCCACAGAGGTCACGCTCAGGTGGTC15460.105277278970489No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15250.10384725124838017No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC15010.10221293385168434No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14860.10119148547874945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)