FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729797
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT23920.3277623777571023No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23340.3198149622429251No Hit
GTGTAAATCTGTGGTTGGCCTGGTATCATCAGAAAGAAGGGGAAGCCCCTAAACTCCTACTTCATACATCGTCCA21420.2935062764028901No Hit
CATCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGGTGTGACA16640.22800861061363636No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT15890.21773178020737274No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15360.21046948672027976No Hit
ATCCTGGAGGGTCCCTGAGACTCACCTGCGAAGCCTCTGGATTCGTCTTT15250.20896221826069442No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15010.20567363253069004No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14060.1926563140160894No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14060.1926563140160894No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13860.18991582590775244No Hit
GTATGATTCGACTCCGTGGACCTTTGGCCAAGGAACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCA13660.18717533779941545No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13590.1862161669614975No Hit
AGTCAGGACACATCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12860.17621338536606754No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12860.17621338536606754No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12680.17374694606856428No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12610.17278777523064634No Hit
GTAATAAATGCCCGTGTCGTCGACTCTGAGACCATTCATCTCTAGAAACAAGGAACTCTTGGCATTGTCTCTGGA12240.16771787223022291No Hit
GTTCAGGGGCAGTGGATCTGGGACAGAATTCAATCTCATCATCGACAGTGTCCAGCCTGAGGATTGTGGAATTGT12180.16689572579772183No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC11850.1623739204189658No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11700.16031855433771308No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT11250.1541524560939549No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT11050.1514119679856179No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10820.14826040666103038No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10730.14702718701227876No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG10700.1466161137960282No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10560.1446977721201923No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC10370.1420943084172722No Hit
ATGTATGAAGTAGGAGTTTAGGGGCTTCCCCTTCTTTCTGATGATACCAGGCCAACCACAGATTTACACTGTGA10270.1407240643631037No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT10260.14058703995768687No Hit
GATTTACACTGTGACTGGCCCGGCAGGTAATGGCGACTCTGTCTCCCACATATGCGGACACGACGTCAGGAGA10070.13798357625476673No Hit
CTTCATACATCGTCCACTTTAAATATTGGGGTCCCGTCAAGGTTCAGGGGCAGTGGATCTGGGACAGAATTCAA9840.1348320149301792No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA9840.1348320149301792No Hit
CTATTATATGAATTGGGTCCGCCAGATCCCAGGGAAGGGGCTGGAATTTGTCTCATACATCAGTAATGGAAGTGG9770.13387284409226127No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9680.13263962444350963No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9220.1263365017943346No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9120.1249662577401661No Hit
GGACACATCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGGTG9010.12345898928058077No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9010.12345898928058077No Hit
GTGTCCAGCCTGAGGATTGTGGAATTGTTTATTGTCAACAGTATGATTCGACTCCGTGGACCTTTGGCCAAGGAA8930.12236279403724597No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8910.12208874522641228No Hit
GACCCAGTCAGGACACATCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG8900.12195172082099542No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTTC8840.12112957438849432No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGGTGTGACATCCAGATGACCCAGTCTCCTGACGTCG8770.1201704035505764No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8630.11825206187474052No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC8620.11811503746932367No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG8590.1177039642530731No Hit
GAACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCATC8570.11742991544223941No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTTCCTTGGCCAAAGGTC8540.11701884222598886No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8520.11674479341515517No Hit
GAATCATACTGTTGACAATAAACAATTCCACAATCCTCAGGCTGGACACTGTCGATGATGAGATTGAATTCTGTC8370.11468942733390244No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8140.11153786600931492No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8140.11153786600931492No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8130.11140084160389807No Hit
GAATTCAATCTCATCATCGACAGTGTCCAGCCTGAGGATTGTGGAATTGTTTATTGTCAACAGTATGATTCGAC7850.1075641582522263No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7800.10687903622514205No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7770.10646796300889151No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7770.10646796300889151No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG7750.10619391419805782No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7580.10386449930597139No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7560.1035904504951377No Hit
CTTTAAATATTGGGGTCCCGTCAAGGTTCAGGGGCAGTGGATCTGGGACAGAATTCAATCTCATCATCGACAGTG7560.1035904504951377No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC7550.10345342608972086No Hit
ATCCAGAGACAATGCCAAGAGTTCCTTGTTTCTAGAGATGAATGGTCTCAGAGTCGACGACACGGGCATTTATTA7500.1027683040626366No Hit
CTCTGTGAGGGGCCGGTTCACCATATCCAGAGACAATGCCAAGAGTTCCT7490.10263127965721974No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTG7470.10235723084638605No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCAGGAGAAGGTGTCCCCCTTCTTCCAG7450.10208318203555236No Hit
CCCCTAAACTCCTACTTCATACATCGTCCACTTTAAATATTGGGGTCCCGTCAAGGTTCAGGGGCAGTGGATC7410.10153508441388495No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAGTATGCTGGTCACAGCGAAGGTGG7360.10084996238680072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGA200.001276030880.520269
AATCGAG400.001829562573.5222570
GCTGATA500.00443048658.817870
CCGTAAC200.006958928452.48475658
GTCGTAT200.00756214451.3929139
ACCCGAG600.00911258549.0148370
GTGGTAT1600.048.8397331
CAACTAG3600.046.29178670
TCGTATG2400.045.70469740
CGTATGC2500.043.92206641
GCCGTCT2450.043.641446
ACGAGAC2700.041.545522
CTTATAC2800.041.255921
TATACAC2900.040.996443
TTATACA2850.040.52382
CCGTCTT2700.039.62809847
ACTAGGC2850.039.39130827
TAGGCAT2950.039.2523229
TCTCCGA3050.038.98022511
CGTCTTC2850.038.7885648
Produced by FastQC (version 0.11.5)