FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729797
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23420.32091115748625987No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23400.32063710867542616No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA20830.285421836483296No Hit
GTGTAAATCTGTGGTTGGCCTGGTATCATCAGAAAGAAGGGGAAGCCCCTAAACTCCTACTTCATACATCGTCCA18040.24719202737199525No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16550.22677539096488475No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT14330.19635597296234433No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC14060.1926563140160894No Hit
CATCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGGTGTGACAT13980.1915601187727546No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC13380.1833386544477437No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA13320.1825165080152426No Hit
ATCCTGGAGGGTCCCTGAGACTCACCTGCGAAGCCTCTGGATTCGTCTTTGGAGACTATTATATGAATTGGGTCC12990.1779947026364866No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12700.17402099487939798No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12650.17333587285231372No Hit
AGTCAGGACACATCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA12510.17141753117647784No Hit
GTAATAAATGCCCGTGTCGTCGACTCTGAGACCATTCATCTCTAGAAACAAGGAACTCTTGGCATTGTCTCTG11780.16141474958104787No Hit
GTATGATTCGACTCCGTGGACCTTTGGCCAAGGAACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT11650.15963343231062885No Hit
GTTCAGGGGCAGTGGATCTGGGACAGAATTCAATCTCATCATCGACAGTG11200.15346733406687063No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11150.1527822120397864No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC11030.15113791917478422No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10920.14963065071519888No Hit
GATTTACACTGTGACTGGCCCGGCAGGTAATGGCGACTCTGTCTCCCACATATGCGGACACGACGTCAGGA10880.1490825530935315No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA10350.14182025960643851No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10270.1407240643631037No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10240.14031299114685317No Hit
ATGTATGAAGTAGGAGTTTAGGGGCTTCCCCTTCTTTCTGATGATACCAGGCCAACCACAGATTTACACTGT10190.13962786911976893No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT10070.13798357625476673No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC9950.13633928338976456No Hit
CTTCATACATCGTCCACTTTAAATATTGGGGTCCCGTCAAGGTTCAGGGGCAGTGGATCTGGGACAGAATTCAAT9720.13318772206517704No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA9570.1311323559839243No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9550.1308583071730906No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA9500.13017318514600634No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9440.12935103871350526No Hit
GGACACATCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGGTGT9290.12729567263225253No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG9180.1257884041726672No Hit
CTATTATATGAATTGGGTCCGCCAGATCCCAGGGAAGGGGCTGGAATTTG8980.12304791606433022No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG8850.12126659879391118No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8800.12058147676682694No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8650.11852611068557421No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8570.11742991544223941No Hit
GACCCAGTCAGGACACATCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8470.11605967138807094No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTTC8340.1142783541176519No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTTCCTTGGCCAAAGGTC8200.11236001244181602No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8110.11112679279306438No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7990.1094824999280622No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG7980.10934547552264534No Hit
GTGTCCAGCCTGAGGATTGTGGAATTGTTTATTGTCAACAGTATGATTCGACTCCGTGGACCTTTGGCCAAGGAA7940.10879737790097796No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7900.10824928027931055No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7900.10824928027931055No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGGTGTGACATCCAGATGACCCAGTCTCCTGACGTCGT7830.10729010944139261No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7800.10687903622514205No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC7700.10550879217097357No Hit
CTTTAAATATTGGGGTCCCGTCAAGGTTCAGGGGCAGTGGATCTGGGACA7680.10523474336013988No Hit
GAACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCATC7630.10454962133305562No Hit
GAATTCAATCTCATCATCGACAGTGTCCAGCCTGAGGATTGTGGAATTGTTTATTGTCAACAGTATGATTCGACT7570.10372747490055455No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC7470.10235723084638605No Hit
GAATCATACTGTTGACAATAAACAATTCCACAATCCTCAGGCTGGACACTGTCGATGATGAGATTGAATTCTGTC7400.1013980600084681No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)