FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791538
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85661.082196938112889No Hit
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC32900.41564650086287713No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30370.3836834112828443No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA29430.3718077969724764No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26800.33858134416793634No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25530.32253663121669457No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24930.31495645186965127No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA23950.3025754922694804No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT23910.30207014697967755No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22930.2896891873795068No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA22140.2797086179058997No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG19660.24837720993812046No Hit
CTACTACTGTTACTCAACAGACAGCAGTGGTAATCATAGAGCGGTGTTCG19170.24218673013803507No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18990.23991267633392205No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT18570.23460655079099174No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG17610.22247826383572236No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC17250.21793015622749634No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16480.20820225939879072No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT15970.20175910695380386No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15920.20112742534155026No Hit
CCCCTGTGCTGGTCATCTATGAGGACACCAAGCGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGTTCAG15890.20074841637419807No Hit
GACTACTACTGTTACTCAACAGACAGCAGTGGTAATCATAGAGCGGTGTTCGGCGGAGGGACCAAGCTGACCGTC15890.20074841637419807No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15770.19923238050478942No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGCTCTATGATTACCACTGCTGTCTGTT15500.1958212997986199No Hit
GTTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAGGCTGACTACTACTGTTACTCAA15300.19329457334960545No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG15010.18963081999853448No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG14810.18710409354952004No Hit
CAGTAGTAGTCAGCCTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGGCCATTGTCCCTGAACTGGAGCCA14470.1828086585861955No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14260.1801555958147303No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC13920.17586016085140577No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT13830.17472313394934924No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13530.17093304427582756No Hit
TCTATGAGGACACCAAGCGACCCTCCGGGATCCCTGAGAGATTCTCTGGC13510.17068037163092611No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATT13300.16802730885946093No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13150.16613226402270012No Hit
GGATGAGGCTGACTACTACTGTTACTCAACAGACAGCAGTGGTAATCATAGAGCGGTGTTCGGCGGAGGGACCAA13080.16524790976554504No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13000.16423721918593928No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATGAGGACACCAAGCGACCCTCCGG12480.1576677304185017No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC12430.15703604880624808No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12180.15387764074498003No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG12060.15236160487557138No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12000.15160358694086704No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGG11920.15059289636126125No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG11720.1480661699122468No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA11540.14579211610813378No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG11470.14490776185097873No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG11430.14440241656117583No Hit
GTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC11300.14276004436931644No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAG11260.14225469907951357No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATTC11250.14212836275706284No Hit
GTGCTGGTCATCTATGAGGACACCAAGCGACCCTCCGGGATCCCTGAGAG11210.14162301746725994No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC11180.14124400849990776No Hit
GTAGTAGTCAGCCTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGGCCATTGTCCCTGAACTGGAGCCAGA10990.13884361837334405No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACG10980.13871728205089331No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10780.13619055560187887No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10740.13568521031207598No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT10730.13555887398962527No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGT10560.13341115650796298No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTG10480.1324004659283572No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGCTCTATGATTA10310.1302527484466949No Hit
GCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAACACGCATATTTTTTTGGCAATGAATCTCCAGAGCAGGT10220.1291157215446384No Hit
TCATAGAGCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACT10210.1289893852221877No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCA10180.1286103762548355No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC9790.12368325967925735No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC9680.12229356013229939No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAA9590.1211565332302429No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9570.12090386058534144No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGG9540.12052485161798927No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC9450.11938782471593277No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT9410.1188824794261299No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC9240.11673476194446761No Hit
GGTGGAGGATGAGGCTGACTACTACTGTTACTCAACAGACAGCAGTGGTAATCATAGAGCGGTGTTCGGCGGAGG9170.11585040768731256No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTA9120.11521872607505894No Hit
GACTATCAGTGGGGCCCAGGTGGAGGATGAGGCTGACTACTACTGTTACTCAACAGACAGCAGTGGTAATCATA9090.11483971710770677No Hit
GTCCCTGAACTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGCTTGGTGTCCTCATAGATGACCAGCACA9030.11408169917300243No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC9020.11395536285055172No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA8870.11206031801379086No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8680.10965992788722714No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTAT8670.10953359156477642No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC8640.10915458259742425No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT8620.1089019099525228No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCA8600.10864923730762137No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGCT8520.10763854672801558No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8470.10700686511576198No Hit
GGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGT8430.1065015198259591No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG8310.10498548395655041No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA8290.10473281131164897No Hit
CCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAGGCTGACTACTACTGT8150.10296410279733885No Hit
GGTAATCATAGAGCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACG8140.10283776647488814No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGGT455.620823E-498.948970
AAGAGGT652.1147214E-591.3374570
CTCGTAT650.002420653668.5030870
CTTGGTT750.0042676659.3693470
GTACGTT253.0397763E-453.3412514
TCTAGGG350.00981106148.11352569
GGTAGAA19300.044.60390570
GCTTGGG752.3646862E-1144.5072861
TGTTGGT11750.041.68485370
GTATCAA41700.040.584881
AAGCGGT11400.040.36073770
TGACAGT26900.040.27845470
GGTATCA15550.039.2837641
AACAGTA11950.037.2610970
GACAGGT1101.8189894E-1236.4150541
GTGGTAT5450.035.5241621
TATCAAC47950.035.113442
GTACGTC1052.3646862E-1134.93685520
TGGTATC5550.034.842212
CGTCCTA18550.034.4054170
Produced by FastQC (version 0.11.5)