FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127276
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTTTGGGAGTCCACCTTACAATTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGT41710.37000699030228623No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA37570.33328129047367283No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35080.31119264492457926No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34330.3045394384338884No Hit
GTATTATTGTGTGAGGTACACCAGCTCATTGGAGGCGGGTGGTCTTGACTATTGGGGCCAGGGAACCCTGGTCAC31910.283071758823926No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28190.25007185463009945No Hit
CAATAATACAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACACCGTGTTCTTGGAATTGTCTCTG25030.22203967794932208No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC24910.22097516491081157No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA24760.21964452361267336No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA23430.20784617076918163No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT23190.20571714469216057No Hit
GTGTATTACTGTCAGCAGTTTGGGAGTCCACCTTACAATTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA19470.17271724049833403No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA19350.1716527274598235No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19170.17005595790205771No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA18990.1684591883442919No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18390.16313662315173924No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC16910.15000762901010933No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC16730.14841085945234353No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA16630.14752376525358477No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16030.1422012000610321No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14600.12951575301878157No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14470.12836253056039515No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT14450.1281851117206434No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14400.12774156462126401No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13770.1221528711690837No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT13680.12135448639020081No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13440.11922546031317974No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12770.11328192918149593No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12660.11230612556286128No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12340.10946742412683318No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA12070.10707226979018448No Hit
GTTTGGGAGTCCACCTTACAATTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT11800.10467711545353578No Hit
GTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCACCTTCTTAGCCTG11540.10237067053676296No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGGTGGATGCACCGTAGATGAGAAGCCTG11450.10157228575788006No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11420.10130615749825242No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11390.10104002923862479No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCACCTTCTTAGCCTGGTACCAGCACAAACCTGGCCAGCCTCCCAG11380.10095131981874891No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG11280.10006422561999014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTTT2508.54925E-1152.42267670
TATTCTA3250.049.2862770
ATTTAAT3151.6370905E-1146.22810470
TAAACCG306.941267E-445.1203885
GTGATTT1654.3587122E-544.1268370
GTGGTAT3850.042.2223321
GGTATCA11200.041.122791
TAGGCAA2154.353373E-640.6377370
GTATCAA31650.040.4463921
TAATCGT3351.5079422E-939.121470
GAGCAAA25250.038.63937470
TGGTATC4150.037.5113832
GGTACGT551.1752291E-437.3052268
AAGCGGT12050.036.2535870
GAGCCAT14250.035.76595770
GTACGGT400.002847882733.8553114
GAACTGT18350.033.78128469
GCTGACA10400.033.60427570
ATCAACG38350.033.3534853
AATGGTA1750.003091195633.28423770
Produced by FastQC (version 0.11.5)