FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974974
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACAAACCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG32720.3355986928882206No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32710.33549612605054085No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT32560.3339576234853442No Hit
GGGTTTGTAGAGCTTGCATGCAGTAATAAATCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAA31650.3246240412564848No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATT28650.2938539899525526No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT23490.2409295017097892No Hit
GTCTCAACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCA22030.22595474340854219No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21990.22554447605782307No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGGTCCTGATCTATTTGCGT21920.22482650819406466No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGCCTC21650.22205720357671077No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGC20760.21292875502321087No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20590.21118511878265472No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19590.20092843501467733No Hit
GTCTCTGGTGGCTCCATCGGCAGTGGTAATTACTACTGGAGCTGGATCCGGCAGGCCGCCGGGAAGGGAC19500.20000533347555935No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19250.19744116253356503No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18740.19221025381189652No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC18480.18954351603222241No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18420.18892811500614376No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18330.18800501346702578No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC18300.18769731295398645No Hit
GCTAATGCTCTGGGCCTCTGGATCCAGTGGAGAAATTGTGATGACTCAGTCTCAACTCTCCCTGCCCGTCACCC18110.18574854303807076No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17890.18349207260911574No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17210.1765175276468911No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17010.17446619089329563No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16990.17426105721793606No Hit
GGCCAAAAGTGTACGGGGTTTGTAGAGCTTGCATGCAGTAATAAATCCCAACATCCTCAGCCTCCACTCTGCTGA15310.15702982848773403No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGCCTCTGGATCCAGTGGAGAAATTGTGATGA15150.15538875908485766No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT15110.15497849173413855No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14930.15313228865590262No Hit
GAAATACACGGCCGTGTCTGCGGCGGTCACAGAGTTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCCATTGA14890.15272202130518353No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC14660.15036298403854872No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT14530.14902961514871166No Hit
GTAATAAATCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA14530.14902961514871166No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAACGCAAATAGA14020.14379870642704318No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGCCTCTGGATCCAGTGGAGAAATTGTGATGACTCAGTCTCAACTCTCC14020.14379870642704318No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG13930.14287560488792522No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13700.14051656762129042No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13510.1385677977053747No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTTGAGACTGAGTCATCACAATTTCTCCACTGGATCCAGAGGCCCAGAGCA13420.13764469616625674No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGA13260.13600362676338035No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACGCAAATAGATCAGGACCTGTGGAGACTGCCCTGGCTTCTGCAGGTA13040.13374715633442533No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12990.13323432214602646No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGATTTATTACTGCATGCAAGCTCTACAAACC12830.13159325274315006No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12830.13159325274315006No Hit
AGCCAGGGCAGTCTCCACAGGTCCTGATCTATTTGCGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAG12760.13087528487939165No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12670.1299521833402737No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12590.1291316486388355No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGGGTTTG12450.12769571291131865No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12350.1266700445345209No Hit
GTCCCTCACCTGCTCTGTCTCTGGTGGCTCCATCGGCAGTGGTAATTACTACTGGAGCTGGATCCGGCAGGCCGC12230.12543924248236363No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11840.12143913581285244No Hit
GGAATGGATTGGGCGTATTTATACCAGTGGAAGCACCAACTACAACCCCT11780.12082373478677381No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11750.12051603427373449No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11450.11743902914334126No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11410.11702876179262216No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11390.11682362811726262No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11390.11682362811726262No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11300.11590052657814463No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGCCTCTGGATCCAGTGGAGAAATTGTGATGACTCAGTCTCAAC11190.11477229136366714No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG11170.11456715768830758No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11170.11456715768830758No Hit
CCACAGGTCCTGATCTATTTGCGTTCTAATCGGGCCTCCGGGGTCCCTGA11140.11425945717526825No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTTGAGACTGAGTCATCACAA11110.11395175666222895No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA10950.11231068725935256No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTTCTCTATAGCA10840.11118245204487505No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC10770.11046448418111662No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGATTTA10760.11036191734343685No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10710.109849083155038No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10660.1093362489666391No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG10520.10790031323912228No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG10510.10779774640144249No Hit
CTACAACCCCTCCCTCGAGAGTCGAGTCACCATATCAATGGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAA10460.10728491221304362No Hit
GGGTTCCCTGGCCCCAGTAGTCTTCTCTATAGCATCTGGTACGACTACAA10390.10656694434928521No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA10380.10646437751160544No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGCCTCTGGATCCAGTGGAGAAAT10380.10646437751160544No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA10230.10492587494640881No Hit
TCTCTATAGCATCTGGTACGACTACAATCCTCCTTCTCTCTCGCACAGAA10160.10420790708265042No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTACTATGC9840.10092576827689766No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC9760.10010523357545946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)