FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974974
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGTAGAGCTTGCATGCAGTAATAAATCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT33340.3419578368243666No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32240.33067548467959146No Hit
CTACAAACCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG30830.3162135605667433No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA29980.30749537936396254No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTG24120.24739121248361495No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT22420.22995485007805336No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGCCTCTG21530.22082640152455346No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21390.21939046579703667No Hit
GTCTCAACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT21250.21795453006951981No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGGTCCTGATCTATTTGCGTT20610.21139025245801427No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC20380.20903121519137946No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGC20110.20626191057402554No Hit
GTCTCTGGTGGCTCCATCGGCAGTGGTAATTACTACTGGAGCTGGATCCG19760.20267207125523348No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17870.18328693893375617No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC17510.17959453277728432No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT17360.17805603021208768No Hit
GCTAATGCTCTGGGCCTCTGGATCCAGTGGAGAAATTGTGATGACTCAGTCTCAACTCTCCCTGCCCGTCACCC16570.16995325003538558No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16480.16903014849626757No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16280.1669788117426721No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA15900.1630812719108407No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC15550.15949143259204862No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC15470.15867089789061042No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG15070.15456822438341947No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA14990.15374768968198127No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14810.15190148660374533No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGCCTCTGGATCCA14680.15056811771390827No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14670.1504655508762285No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC14510.1488244814733521No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGCCTCTGGATCCAGTGGAGAAATTGTGAT14390.1475936794211948No Hit
GAAATACACGGCCGTGTCTGCGGCGGTCACAGAGTTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCCATT14250.14615774369367798No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA14130.1449269416415207No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14040.14400384010240275No Hit
GGCCAAAAGTGTACGGGGTTTGTAGAGCTTGCATGCAGTAATAAATCCCAACATCCTCAGCCTCCACTCTGCT14030.14390127326472296No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAACGCAAATA14010.14369613958936342No Hit
GTAATAAATCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA13980.1433884390763241No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13700.14051656762129042No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13680.14031143394593087No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGCCTCTGGATCCAGTGGAGAAATTGTGATGACTCAGTCTCAACTCTCC13500.13846523086769494No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA13270.13610619360106013No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT13230.13569592625034102No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGATTTATTACTGCATGCAAGCTCTACAAACC12930.13261892111994783No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG12810.13138811906779055No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTTGAGACTGAGTCATCACAATTTCT12650.12974704966491415No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12580.12902908180115574No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12160.12472127461860522No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACGCAAATAGATCAGGACCTGTGGAGACTGCCCTGGCTTCTGCAGGTA12080.12390073991716702No Hit
GGAATGGATTGGGCGTATTTATACCAGTGGAAGCACCAACTACAACCCCT11730.12031090059837493No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11710.12010576692301539No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11710.12010576692301539No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG11620.11918266538389741No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11560.11856726435781878No Hit
GTCCCTCACCTGCTCTGTCTCTGGTGGCTCCATCGGCAGTGGTAATTACTACTGGAGCTGGATCCGGCAGGCCGC11550.11846469752013901No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGAT11460.11754159598102103No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11260.11549025922742555No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11170.11456715768830758No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11080.11364405614918963No Hit
AGCCAGGGCAGTCTCCACAGGTCCTGATCTATTTGCGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTG10960.11241325409703233No Hit
GGGTTCCCTGGCCCCAGTAGTCTTCTCTATAGCATCTGGTACGACTACAA10880.11159271939559415No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG10870.11149015255791438No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT10590.10861828110288069No Hit
CCACAGGTCCTGATCTATTTGCGTTCTAATCGGGCCTCCGGGGTCCCTGA10520.10790031323912228No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10440.10707977853768409No Hit
TCTCTATAGCATCTGGTACGACTACAATCCTCCTTCTCTCTCGCACAGAA10210.10472074127104929No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG10110.10369507289425153No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTTCTCTATAGCAT10090.10348993921889199No Hit
CTACAACCCCTCCCTCGAGAGTCGAGTCACCATATCAATGGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAA9960.10215657032905494No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9930.10184886981601561No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGCCTCTGGATCCAGTGGAGAAATTGTGATGACTCAGTCTCAACT9900.10154116930297628No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG9820.10072063460153809No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT9780.100310367250819No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)