FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335095
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCATCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTGA56040.4197454113752204No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT49670.3720334508031264No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45350.33967620281702804No Hit
GTCTCTGGTGGCTCCATCAGTGGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGAT37770.28290121676734614No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33150.24829693767110206No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG32420.24282916196974746No Hit
GAGCTACAGTTCCCCGTGGACGTTCGGCCCAGGGGCCAAGGTGGAAATCAAACGAACTGTGGCTGCACCA31460.23563866241728118No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT30210.22627603279167402No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29610.22178197057138258No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGTAGTAGATCTGGGACAGATTTCA28970.21698830420307172No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27580.20657706005939655No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27390.20515394035630422No Hit
GTGTATTACTGTGCGAGAGACTCGGAGGAAAGTACCAGTCTCTACTACTA26760.20043517502499822No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGAGCATTAGCACCAATTTAAATTGGTATCAGCAGAAA25930.19421838895359506No Hit
GGGTATATCTATGACAGTGGGACCACCAAGTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACG25240.1890502174002599No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC25210.18882551428924535No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25100.1880016028821919No Hit
ATCTATGACAGTGGGACCACCAAGTACAACCCCTCCCTCAAGAGTCGAGT24390.18268362925484705No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24190.18118560851474988No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24050.1801369939966819No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGAGCATTAGCAC24020.17991229088566732No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG23890.17893857740460414No Hit
GTCATAGATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGATCCAGCTCCAGTAGTAACCACTGA23860.1787138742935896No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23520.17616723903542444No Hit
CTGTAGCTCTGTTGACAGTAGTAAGTTGCTAAATCTTCAAGTTGCAGACTACTGATGGTGAGAGTGAAATCTGTC23170.17354570274025444No Hit
ACTTACTACTGTCAACAGAGCTACAGTTCCCCGTGGACGTTCGGCCCAGGGGCCAAGGTGGAAATCAAACGAA22830.1709990674820893No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC22800.1707743643710747No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATTGGTGCTAATGCTCTGGCT22720.17017515607503586No Hit
GTAGTAAGTTGCTAAATCTTCAAGTTGCAGACTACTGATGGTGAGAGTGAAATCTGTCCCAGATCTACTACCA22680.16987555192701642No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC22030.1650069845217007No Hit
CTACTGATGGTGAGAGTGAAATCTGTCCCAGATCTACTACCACTGAACCTTGATGGGACCCCACTTTGCAAAC21990.16470738037368127No Hit
CAGTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCATCTTCAGGGAGAACTGGTTCTTGGACGTGTCTA21790.16320935963358413No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21750.1629097554855647No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT21750.1629097554855647No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGCCCCTGGGCCGAACGTC21050.1576666828952247No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20630.15452083934102068No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC20490.15347222482295267No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20110.1506259854167681No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATG19360.1450084076414038No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACA19150.1434354858643018No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18410.13789280912594234No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGCCCCTGGGCCGAACGTCCACGGGGAA18300.13706889771888892No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18260.1367692935708695No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18260.1367692935708695No Hit
GAGTGAAATCTGTCCCAGATCTACTACCACTGAACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCATAGA17720.1327246375726072No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT17520.13122661683251005No Hit
GTATTACTGTGCGAGAGACTCGGAGGAAAGTACCAGTCTCTACTACTACT17450.13070230957347603No Hit
GATTTCACTCTCACCATCAGTAGTCTGCAACTTGAAGATTTAGCAACTTACTACTGTCAACAGAGCTACAGTTCC17270.1293540909073886No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT17030.12755646601927204No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16950.12695725772323319No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG16710.12515963283511658No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGAGCATTAGCACCAATTTAAATTGGTA16340.12238829446593688No Hit
GCCCAGGGGCCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC16190.12126477891086403No Hit
CACTTGCACTGTCTCTGGTGGCTCCATCAGTGGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA16150.12096517476284459No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15790.11826873743066973No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15670.11736992498661145No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15630.117070320838592No Hit
GAACTGTAGCTCTGTTGACAGTAGTAAGTTGCTAAATCTTCAAGTTGCAGACTACTGATGGTGAGAGTGAAA15470.11587190424651428No Hit
GTGGTAGTAGATCTGGGACAGATTTCACTCTCACCATCAGTAGTCTGCAACTTGAAGATTTAGCAACTTACTA15270.11437388350641715No Hit
GTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGATGAG15240.11414918039540256No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC15170.11362487313636858No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14860.111302940989218No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14820.11100333684119856No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14700.11010452439714027No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA14570.10913081091607714No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGTAGTAGATCTGGGACAGAT14480.10845670158303343No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14360.10755788913897513No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14230.10658417565791198No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14190.10628457150989255No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14110.1056853632138537No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14110.1056853632138537No Hit
GTAGTAGATCTGGGACAGATTTCACTCTCACCATCAGTAGTCTGCAACTT13930.10433714454776626No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13900.10411244143675169No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13880.10396263936274198No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13670.10238971758563997No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13560.10156580617858654No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA13520.1012662020305671No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT13410.10044229062351369No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGAG452.1543057E-591.02711570
ATGCGGG500.0037287161.44329570
TGGTCCG550.005437967455.85754470
TAGCGTA200.00787967750.863317
TATTCTA1652.8467184E-949.65115470
TCGTATG2550.048.38419340
CGTATGC2650.046.5971841
ACGAGAC2750.045.65363322
TGTTAGA12850.043.03421870
ATGCCGT2900.042.68691644
GTGGTAT2550.042.5682561
ATCTCGT2700.041.7743137
ACATGCG3000.040.76852427
CGAGACA3000.040.7226823
CGCAGAT2900.039.93471532
ATCTCCG3150.039.82947510
GATCTCG2850.039.5443136
GCGTAGA27850.039.34430370
GCCGTCT3250.038.16814446
CCGTCTT3300.037.63424747
Produced by FastQC (version 0.11.5)