FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335095
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCATCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACT51160.3831937053168501No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46470.348065118961572No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA45150.33817818207693084No Hit
GTCTCTGGTGGCTCCATCAGTGGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATT36160.2708421498095641No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG32560.24387777648781547No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31620.23683707900935888No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31460.23563866241728118No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT29880.22380429857051373No Hit
GAGCTACAGTTCCCCGTGGACGTTCGGCCCAGGGGCCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT28890.2163890959070328No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC28470.21324325235282882No Hit
GTGTATTACTGTGCGAGAGACTCGGAGGAAAGTACCAGTCTCTACTACTACTATTACATGGACGTCTGGGGCAAA27820.2083746849475131No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGTAGTAGATCTGGGACAGATTTCA26470.19826304495185734No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC25970.1945179931016145No Hit
ATCTATGACAGTGGGACCACCAAGTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAA25090.18792670184518703No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA24640.18455615517996846No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGAGCATTAGCACCAATTTAAATTGGTATCAGCAGAAA24410.18283343132885674No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24020.17991229088566732No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23730.17774016081252644No Hit
GGGTATATCTATGACAGTGGGACCACCAAGTACAACCCCTCCCTCAAGAG23380.17511862451735644No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA23070.17279669237020587No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC22920.171673176815133No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC22540.16882693740894844No Hit
CTGTAGCTCTGTTGACAGTAGTAAGTTGCTAAATCTTCAAGTTGCAGACTACTGATGGTGAGAGTGAAATCTGTC22220.16643010422479299No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGAGCATTAGCAC22170.16605559903976871No Hit
ACTTACTACTGTCAACAGAGCTACAGTTCCCCGTGGACGTTCGGCCCAGGGGCCAAGGTGGAAATCAAACGAA22030.1650069845217007No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21290.15946430778334125No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATTGGTGCTAATGCTCTGGCTT21290.15946430778334125No Hit
GTAGTAAGTTGCTAAATCTTCAAGTTGCAGACTACTGATGGTGAGAGTGAAATCTGTCCCAGATCTACTACCA21080.15789138600623925No Hit
CAGTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCATCTTCAGGGAGAACTGGTTCTTGGACGTGTCTA20800.15579415697010324No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20740.1553447507480741No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGT20640.15459574037802554No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20440.1530977196379284No Hit
CTACTGATGGTGAGAGTGAAATCTGTCCCAGATCTACTACCACTGAACCTTGATGGGACCCCACTTTGCAAACTG20440.1530977196379284No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT20200.15130009474981182No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGCCCCTGGGCCGAACGTC20140.15085068852778266No Hit
GTCATAGATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGATCCAGCTCCAGTAGTAACCACTGAT20110.1506259854167681No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTA19480.14590722008546209No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19450.1456825169744475No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA18820.1409637516431415No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18770.1405892464581172No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17920.13422265831270433No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT17800.13332384586864607No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGCCCCTGGGCCGAACGTCCACGGGGAA17260.12927918987038375No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA17250.1292042888333789No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17040.1276313670562769No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16670.12486002868709717No Hit
GATTTCACTCTCACCATCAGTAGTCTGCAACTTGAAGATTTAGCAACTTACTACTGTCAACAGAGCTACAGTTCC16560.12403611728004375No Hit
GAGTGAAATCTGTCCCAGATCTACTACCACTGAACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCATA16420.12298750276197574No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG16260.12178908616989803No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16190.12126477891086403No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC16150.12096517476284459No Hit
GTGGTAGTAGATCTGGGACAGATTTCACTCTCACCATCAGTAGTCTGCAACTTGAAGATTTAGCAACTTACTA16130.12081537268883488No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGA16090.12051576854081544No Hit
GTATTACTGTGCGAGAGACTCGGAGGAAAGTACCAGTCTCTACTACTACTATTACATGGACGTCTGGGGCAAAGG15800.1183436384676746No Hit
GCCCAGGGGCCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC15750.1179691332826503No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATTGGTGCTAATGCTCTGGCTTGCCCG15460.11579700320950943No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15310.11467348765443656No Hit
GTGGGGTCCCATCAAGGTTCAGTGGTAGTAGATCTGGGACAGATTTCACTCTCACCATCAGTAGTCTGCAACTT15230.11407427935839771No Hit
GTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGATGA15170.11362487313636858No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT15110.11317546691433944No Hit
CACTTGCACTGTCTCTGGTGGCTCCATCAGTGGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGG14990.11227665447028114No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14930.11182724824825199No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14850.11122803995221313No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14650.109730019212116No Hit
GAACTGTAGCTCTGTTGACAGTAGTAAGTTGCTAAATCTTCAAGTTGCAGACTACTGATGGTGAGAGTGAAAT14370.10763279017597999No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCA14340.10740808706496542No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14260.10680887876892656No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14120.10576026425085855No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT14100.10561046217684884No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13490.10104149891955255No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13360.10006778543848939No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT650.002374621668.8474270
AATGGTA4050.058.9311370
GTTATTA1052.2214506E-456.82644770
TATTCTA1502.1533699E-549.7231470
GACACGT17950.049.0306270
GTGGTAT3450.046.1709751
GAGCAAA26700.045.25364370
CCAGAGT9100.042.61983570
GACAGGT3250.039.6269381
TAAGTCA1908.6924236E-539.2551170
ACTCAGT12150.038.0596970
TAGGCAA4401.0913936E-1137.29235570
GTATCAA21800.035.9125141
AAGGACA28500.035.59129770
TATTGTT5550.034.94058270
CTCGTAT3009.0567846E-734.806270
TTACTTT2151.7990066E-434.69056370
GAGCCAT12300.033.95726470
ATGGCAA2202.0591824E-433.90213870
TAGTACG400.002849912333.850844
Produced by FastQC (version 0.11.5)