FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067678
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT51510.4824488282047583No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42770.40058894160973624No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA34360.3218198745314598No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31420.2942834824731801No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT30640.286977909069963No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG29910.28014064165413166No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCG28760.26936960394426035No Hit
GTCCCAGACCCACTGCCAGTGAACCTGGCTGGGATGCCAGTGGCCCTGTT27300.2556950691125976No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26980.2526979107933291No Hit
CTCCTAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTC26980.2526979107933291No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCTAGGC25640.24014731033139206No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25530.23911703715914348No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC25470.23855506997428064No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA24940.23359102650799213No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24270.22731572627702362No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23690.22188337682334935No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23650.2215087320334408No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23200.2172939781469694No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC23190.21720031694949224No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22940.21485878701256372No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22610.21176796749581805No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCGTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA21780.20399408810521524No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC21020.19687583709695244No Hit
GCCTAGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTAGCTGCTA20660.19350403398777533No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGACGGAGGCCAGT20640.19331671159282104No Hit
GTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCTAGGCTCCTCATCTATGA20520.19219277722309536No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCGTCTTTCGGCGGAGGGA20360.19069419806346108No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGAC20230.18947660249625822No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCAG19370.18142173951322402No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19370.18142173951322402No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA19310.18085977232836117No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT19140.17926753197124976No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCAGTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGA18850.17655135724441265No Hit
ATAATATACGGCCGTGTCCGCAGCGGTCACAGAACTCAGCTTCAGGGAAAACTGCTCCTTGGACGTGTCTAGTGA18110.16962042863110413No Hit
GTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCT17440.16334512840013563No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGACTTCAC17080.1599733252909585No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16900.15828742373636995No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16110.15088818913567573No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC15930.1492022875810872No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15900.14892130398865575No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC15790.1478910308164072No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15760.14761004722397578No Hit
GCGTAGCAACTGGCCTCCGTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA15640.14648611285425006No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15130.14170939178291583No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15120.1416157305854387No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA15010.14058545741319012No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14950.14002349022832727No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC14640.1371199931065359No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14550.13627704232924157No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14140.13243693323267877No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC14130.1323432720352016No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13930.1304700480856588No Hit
GAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCTAGGCTCCTCATCTATGATGCATC13920.13037638688818165No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13660.12794119575377594No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13640.12775387335882166No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13630.12766021216134452No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGACTTCA13510.12653627779161883No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13500.1264426165941417No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13340.12494403743450741No Hit
ACAGAAACCTGGCCAGGCTCCTAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGT13280.12438207024964457No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGT13250.12410108665721314No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13170.12335179707739599No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12780.11969901037578745No Hit
GGGTATATCTATTACAGTGGGAGCACCAGCTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCACTAGACACG12600.11801310882119889No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12560.11763846403129033No Hit
CAATAATATACGGCCGTGTCCGCAGCGGTCACAGAACTCAGCTTCAGGGAAAACTGCTCCTTGGACGTGTCTAG12490.11698283564895035No Hit
GTCTCTGGTGGCTCCATCAGTAATTACTACTGGAGCTGGATCCGACAGCCCCCAGGGAAGGGACTGGAGTGGAT12040.11276808176247895No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC11920.11164414739275326No Hit
TCATAGATGAGGAGCCTAGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTAGCTGCTAACACTCTGAC11590.10855332787600756No Hit
TTATTACTGTCAGCAGCGTAGCAACTGGCCTCCGTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTG11560.10827234428357614No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT11510.10780403829619044No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT11460.10733573230880472No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT11280.10564983075421616No Hit
GAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGT11220.1050878635693533No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCT11200.10490054117439902No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11140.10433857398953618No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11140.10433857398953618No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGG11120.1041512515945819No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTT11120.1041512515945819No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCT11110.10405759039710474No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAC11010.10312097842233334No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10980.10283999482990189No Hit
CAACAGGGCCACTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG10930.10237168884251618No Hit
GGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAAC10900.10209070525008476No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG10840.1015287380652219No Hit
ACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCTAGGCTCCTCATCTATGATGCATCCAACAGGGCCAC10730.10049846489297336No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT10710.10031114249801906No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10690.10012382010306478No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT150.002419537968.53477539
TCGACTT252.458764E-455.68380758
TGTTAGA8850.054.95635670
GCGTATG252.6426013E-454.87698740
TGCGTTA200.007763306651.05441313
CGTATGC2400.048.61882841
TCGTATG2200.046.7701640
GGTATCA8350.045.2521861
CACGAGA3000.043.14494721
TGTCGTA405.2214134E-542.8120338
CCCTAAG2950.041.63915628
GCGTAGA25500.041.32502470
ACGAGAC3150.041.10391222
AACTCTA350.001098752141.10080766
ATGCCGT2950.040.78314644
GTATGCC2950.040.74460242
GCCGTCT2750.040.05610346
TATACAC3300.039.191453
TGCCGTC2900.039.13787545
ATCTCGT2450.039.1239937
Produced by FastQC (version 0.11.5)