FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291868
Sequences flagged as poor quality0
Sequence length18-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38821.3300533117710744No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT7320.250798306083572No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC5330.18261679937505998No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4350.14903997697589322No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGA4270.1462990118820837No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC3750.12848273877232175No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTT3690.1264270149519646No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC3610.12368604985815507No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGAT3600.12334342922142887No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT3580.12265818794797649No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG3530.12094508476434554No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT3260.11169432757273837No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT3170.10861074184220264No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA3140.10758287993202406No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT3030.10381405292803597No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT3020.10347143229130977No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG2980.10210094974440499No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGT150.0052626315160.1144170
AACGTTA405.4123248E-6120.0857970
GTAGGAA500.001977387872.0514870
TCGGTTT1253.880814E-757.64118670
TTAAGTT700.007496202451.46534370
TATGCAA200.008412356550.01859716
GGAATAA1901.3638055E-744.24213870
CGCATAA1456.303545E-541.40889770
TAATCTT1250.001521026638.42745670
CAACGTT550.001674495437.70866469
TACAGTA400.001753669937.3610566
GGTACCT953.1286618E-1035.11882819
TCCCAAA2408.5201646E-735.0250370
GTAGTTT2408.5201646E-735.0250370
GTATCAA21500.034.628051
CAAAGTT600.002566554634.56627769
AGGATCA2501.1724278E-633.62402370
GGACTGA400.0030716633.3285946
GAAACCA1450.003150893433.12711770
GGTATCA9800.032.3637731
Produced by FastQC (version 0.11.5)