FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063778
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTCCTTAGCCTGGTACCAGCAGAA66860.6285145960905377No Hit
GTCTATGGTGGGTCCTTCAGTAATTACTACTGGACCTGGATCCGCCAGTCCCCAGGGAAGGGGCTGGAGTGGAT56740.5333819650340579No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT48610.45695624462998863No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43490.4088259016448921No Hit
CTCCTAAGCTGCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTC40330.3791204555837778No Hit
ATGTTATAGTCCTCCGTACACTTTTGGCCAGGGGACCAAGCTCGAGATCAAACGAACTGTGGCTGCACCA39690.3731041627106408No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGGCAGGGAGTCTGGAGA33890.3185815085478361No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAGGCTAAGGAGTTCTTATTGTTGGAGCTGTATAAAACAC33310.31312924313155566No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT31160.29291825926086085No Hit
CTATAACATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCACGCTGCTGATGGTGAGAGTGAAATCTGTC30700.28859404875829353No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29820.2803216460577301No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27310.25672649744589565No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25990.2443178933950505No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC25460.23933565085948383No Hit
GTTATAGTCCTCCGTACACTTTTGGCCAGGGGACCAAGCTCGAGATCAAACGAACTGTGGCTGCACCATCTGTCT25260.23745555933662849No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24230.22777308799392357No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22880.21508247021465007No Hit
CTTTTGGCCAGGGGACCAAGCTCGAGATCAAACGAACTGTGGCTGCACCA22740.2137664061486513No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22630.21273235581108088No Hit
CAATAATACACAGCCGTATCCGCGGCGAGCAGAGAGCTCAACTTCAGGGAGAACTGGTTCTTGGACGTGTCTAG21960.2064340492095155No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21400.20116979294552056No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21110.19844366023738036No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21060.1979736373566665No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAG20730.19487148634395524No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT20520.19289739024495714No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC19620.18443697839210813No Hit
GTTTATTACTGTCAGCAATGTTATAGTCCTCCGTACACTTTTGGCCAGGGGACCAAGCTCGAGATCAAACGAA19270.1811468182271113No Hit
GTCTCCAGACTCCCTGCCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA18760.1763525848438302No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCGTGCAGGCTGAAGA18670.1755065436585453No Hit
GTGCAGCCACAGTTCGTTTGATCTCGAGCTTGGTCCCCTGGCCAAAAGTG18460.1735324475595472No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18390.17287441552654784No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT18320.17221638349354848No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17850.16779816841483844No Hit
GTATTATTGTGGGAGACTCCGAGGCCAGCCGAAAGCGGGTATAGCAGGAATCAGGAGATACTACTCTCACGGTG17740.16676411807726801No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCTGACCGATTCAG17170.1614058572371303No Hit
GGCCAAAAGTGTACGGAGGACTATAACATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCACGCTGCTGA16770.15764567419141964No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16770.15764567419141964No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16710.15708164673456304No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16440.15454352317870834No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16280.15303944996042407No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16240.152663431655853No Hit
CTCCCTGCCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAA15400.14476704725986062No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG15260.14345098319386188No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCGAGCTTGGTCC15200.14288695573700527No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA15190.14279295116086252No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15160.1425109374324342No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15130.14222892370400592No Hit
CCTTAGCCTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATT14960.14063084590957886No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14840.13950279099586568No Hit
ACCATAGACAGCGCATGTGAGGGACAGGGTCTCCGAAGGCTTCAACTGTCCTGCGCCCCACTGCTGTAGCTGCAC14750.13865674981058076No Hit
GTAATAAACTGCCACATCTTCAGCCTGCACGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA14660.13781070862529587No Hit
GATTTCACTCTCACCATCAGCAGCGTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATGTTATAGTCC14620.1374346903207248No Hit
GGACTATAACATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCACGCTGCTGATGGTGAGAGTGAAA14250.13395652100344246No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCGTGCAGGCTGAAGATGTGGCAGTTTA14230.13376851185115693No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14210.13358050269887137No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14200.13348649812272861No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC14170.1332044843943003No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14100.13254645236130094No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATGTTATAGTCCTCCGTACACTTTTGGCCAGGGGACCAAGC14100.13254645236130094No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG14050.13207642948058712No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13760.1293502967724469No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13360.12559011372673623No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13170.12380402678002365No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13050.12267597186631045No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA13020.12239395813788215No Hit
CCATCAGCAGCGTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATGTTATAGTCCTCCGTACACTTT12850.12079588034345512No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12730.11966782542974191No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12490.11741171560231553No Hit
GTCCTGGTTTCTGCTGGTACCAGGCTAAGGAGTTCTTATTGTTGGAGCTG12420.11675368356931616No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12190.11459157831803252No Hit
GTGTGGACGTCTGGGGCCAAGGGAGCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG12130.11402755086117593No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG12110.1138395417088904No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12050.11327551425203378No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12040.11318150967589101No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGCCTGTGTCTCTGGGCGAGAGGGCCACCATCAAC11870.11158343188146398No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG11870.11158343188146398No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC11810.11101940442460739No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT11690.10989134951089419No Hit
CCCTTGGCCCCAGACGTCCACACCGTGAGAGTAGTATCTCCTGATTCCTG11440.10754123510732504No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11440.10754123510732504No Hit
CACCATCAGCAGCGTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATGTTATAGTCCTCCGTACACTTT11280.10603716188904075No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC11280.10603716188904075No Hit
ATAATACACAGCCGTATCCGCGGCGAGCAGAGAGCTCAACTTCAGGGAGAACTGGTTCTTGGACGTGTCTAGTGA11250.10575514816061246No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11180.10509711612761308No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11150.1048151023991848No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT11120.10453308867075649No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG11030.1036870474854716No Hit
GTCAGGGACCCCGGATTCCCGGGTAGAAGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA10960.10302901545247223No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10860.10208896969104457No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCGTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAA10860.10208896969104457No Hit
GTGTATTATTGTGGGAGACTCCGAGGCCAGCCGAAAGCGGGTATAGCAGGAATCAGGAGATACTACTCTCACGG10860.10208896969104457No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10790.10143093765804519No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10730.1008669102011886No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10710.10067890104890305No Hit
TCTTCAGCCTGCACGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC10680.10039688732047478No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10640.1000208690159037No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTC208.6137385E-568.83761645
CGTATGC1100.062.3441441
GGAATAA701.8687923E-458.85170470
TCGTATG1050.058.72007440
GGCGAAT1150.056.17816532
TATCGTA252.6860452E-454.69608738
GCGAATC1000.054.44012533
CCCCGTT200.00616063354.12847565
ATCTCGT950.053.9379837
ACTAAGG1450.053.7750827
CCGTCTT1200.051.76931447
GTATGCC1400.051.4903342
AGACTAA1450.051.39346325
TAAGGCG1600.050.88367529
GCCGTCT1100.050.1308446
GACTAAG1500.049.6920426
ATGCCGT1400.049.11570744
AATCTCG1000.047.76833336
TACACAT1850.047.549315
CACGAGA1650.047.1924321
Produced by FastQC (version 0.11.5)