FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063778
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTCCTTAGCCTGGTACCAGCAGAA57010.5359200885899126No Hit
GTCTATGGTGGGTCCTTCAGTAATTACTACTGGACCTGGATCCGCCAGTCCCCAGGGAAGGGGCTGGAGTGGATT56790.5338519879147717No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47080.4425735444801453No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA42160.3963232930179041No Hit
CTCCTAAGCTGCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTG36850.34640686308609503No Hit
ATGTTATAGTCCTCCGTACACTTTTGGCCAGGGGACCAAGCTCGAGATCAAACGAACTGTGGCTGCACCAT36020.3386044832662454No Hit
CTATAACATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCACGCTGCTGATGGTGAGAGTGAAATCTGTC31780.2987465429817124No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGGCAGGGAGTCTGGA29690.2790995865678741No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT29570.27797153165416094No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29340.27580942640287726No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAGGCTAAGGAGTTCTTATTGTTGGAGCTGTATAAAACACT28840.271109197595739No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC26980.2536243464331843No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC25900.24347185220976558No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25520.23989967831634043No Hit
GTTATAGTCCTCCGTACACTTTTGGCCAGGGGACCAAGCTCGAGATCAAA24410.22946517036449335No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC22150.20822013615622806No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20820.1957175275292401No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA20810.19562352295309735No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20060.18857317974238988No Hit
CAATAATACACAGCCGTATCCGCGGCGAGCAGAGAGCTCAACTTCAGGGAGAACTGGTTCTTGGACGTGTCTAGT19990.1879151477093905No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19750.1856590378819641No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19210.1805827907702547No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA19080.17936073128039873No Hit
CTTTTGGCCAGGGGACCAAGCTCGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT19060.1791727221281132No Hit
GTGCAGCCACAGTTCGTTTGATCTCGAGCTTGGTCCCCTGGCCAAAAGTG18830.17701061687682956No Hit
GTATTATTGTGGGAGACTCCGAGGCCAGCCGAAAGCGGGTATAGCAGGAATCAGGAGATACTACTCTCACGGTGT18740.17616457569154467No Hit
GTAATAAACTGCCACATCTTCAGCCTGCACGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA18470.17362645213568997No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT18300.17202837434126295No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18010.1693022416331227No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18000.16920823705697993No Hit
GTTTATTACTGTCAGCAATGTTATAGTCCTCCGTACACTTTTGGCCAGGGGACCAAGCTCGAGATCAAACGAA18000.16920823705697993No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17700.16638809977269695No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA17620.1656360631635548No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17480.16431999909755607No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA16980.15961977029041774No Hit
GGCCAAAAGTGTACGGAGGACTATAACATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCACGCTGCT16940.15924375198584667No Hit
TCTTCAGCCTGCACGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC16350.15369748199342345No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16290.15313345453656685No Hit
GTCTCCAGACTCCCTGCCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA16150.1518173904705681No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16020.15059533098071212No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15690.14749317996800085No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCGTGCAGGCTGAAGAT15410.1448610518360034No Hit
GGACTATAACATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCACGCTGCTGATGGTGAGAGTGAAAT15390.14467304268371783No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15350.14429702437914677No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGAAGCCCAGTAAATGAGCAGCTTA14990.14091285963800718No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG14820.13931478184358015No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTA14790.13903276811515183No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC14630.1375286948968676No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATGTTATAGTCCTCCGTACACTTTTGGCCAGGGGACCAAGCT14530.13658864913543992No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14470.1360246216785833No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC14460.13593061710244053No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTG14210.13358050269887137No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT13980.13141839744758774No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13850.13019633795773178No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG13810.1298203196531607No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13700.12878626931559028No Hit
ACCATAGACAGCGCATGTGAGGGACAGGGTCTCCGAAGGCTTCAACTGTCCTGCGCCCCACTGCTGTAGCTGCAC13620.12803423270644815No Hit
CTCCCTGCCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAA13600.1278462235541626No Hit
GATTTCACTCTCACCATCAGCAGCGTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATGTTATAGTCCT13460.1265301594881639No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13230.12436805423688024No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13070.12286398101859598No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCG12810.12041986203888407No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG12400.11656567441703063No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12260.11524961035103189No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT12240.11506160119874635No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12190.11459157831803252No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC12090.11365153255660486No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG12030.11308750509974827No Hit
GTGTGGACGTCTGGGGCCAAGGGAGCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCC12000.11280549137131995No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11930.1121474593383206No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11800.11092539984846463No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11780.11073739069617909No Hit
CCTTAGCCTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCTTCTACCCGGGAATCCG11760.11054938154389354No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11710.11007935866317972No Hit
GTCAGGGACCCCGGATTCCCGGGTAGAAGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA11620.10923331747789483No Hit
GTCCTGGTTTCTGCTGGTACCAGGCTAAGGAGTTCTTATTGTTGGAGCTG11460.10772924425961054No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA11380.10697720765046842No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11270.10594315731289798No Hit
CCATCAGCAGCGTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATGTTATAGTCCTCCGTACACTTTTG11240.1056611435844697No Hit
CACCATCAGCAGCGTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC11010.10349903833318605No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCGAGCTTGGTCC11010.10349903833318605No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC10980.10321702460475775No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC10720.10077290562504583No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGATT200.0060910727152.5365370
AATGGTA2350.077.8909970
GGCGTGT800.004946856357.201270
TAGCGTA200.007678129751.1970437
AGAATTT900.00788256950.84551270
TAACGGC352.439543E-548.6989236
GTGGATT16300.046.31934469
AAAGTCA1703.951489E-544.8636870
CTGCTCA47600.042.94095670
ACTTCCA3206.1118044E-1042.90089870
GGTATCA6900.042.628061
GAGCAAA21550.041.05391370
GTGGTAT2400.040.851891
GTATCAA18700.039.9554521
AGTAAAT13800.039.7921470
GAGCCAT13650.039.11192770
TGGTATC2550.038.403652
AAGCGGT11450.035.9693170
CTATCTA400.00250450134.75394452
AGTGGAT16650.034.41618368
Produced by FastQC (version 0.11.5)