FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1576385
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA120580.7649146623445415No Hit
GTCTATTACTGTGCGAGAGACCGGTCAGGGCTCACCCCTACTTTCTTTGA67250.426608981942863No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT40370.2560922617254034No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA38590.2448006039133841No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38400.2435953145963708No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT37730.23934508384690287No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT33740.21403400818962373No Hit
GTATATGTAACTACTACTACTACTAATGGATGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGT33510.2125749737532392No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGG33290.2111793755966975No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATAGCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG30860.19576435959489594No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC29750.18872293253234457No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA29650.18808856973391652No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG29390.1864392264580036No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC28820.1828233585069637No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC28720.18218899570853567No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC28490.18072996127215116No Hit
CAGTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTGGCGTTGTCTC26920.17077046533683077No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26690.16931143090044629No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT26540.1683598867028042No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG26360.1672180336656337No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG26180.16607618062846324No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGG25850.16398278339365066No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC25830.16385591083396506No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA25570.16220656755805213No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA25180.15973255264418273No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24360.1545307776970727No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT23420.14856776739184907No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23340.14806027715310663No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC23270.147616223194207No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG23200.14717216923530735No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23110.14660124271672212No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT22730.1441906640826955No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG22680.14387348268348152No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22250.1411457226502409No Hit
CCTTGGGGCTGGTCGGGGATGCAGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGAAAGTAGGGG22080.14006730589291322No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC22050.1398769970533848No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTCTATTA22040.13981356077354198No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG21860.1386717077363715No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21710.13772016353872943No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA21510.13645143794187334No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGC21360.13549989374423127No Hit
AGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC21350.13543645746438845No Hit
TACATATACTATGCAGACTCATTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATC21210.13454834954658917No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21050.13353336906910432No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC20770.13175715323350579No Hit
ATACTATGCAGACTCATTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAA20770.13175715323350579No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20700.13131309927460613No Hit
GTCTGGGGGAGGCCTGGTCAAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAG20680.13118622671492053No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG20600.1306787364761781No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGAAAGTAGGGGTGAGCCCTGACCGGTCTCTCGCACAGTAATAGACAGCCG20550.13036155507696406No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG20510.13010780995759286No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC20230.1283315941219943No Hit
CTATTACTGTGCGAGAGACCGGTCAGGGCTCACCCCTACTTTCTTTGACTACTGGGGCCAGGGAACCCTGGTCAC20150.12782410388325186No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCATTCACTTTCGGCCC20150.12782410388325186No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20050.1271897410848238No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGCTATAGCTAC19780.1254769615290681No Hit
GAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAG19280.12230514753692784No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCATTCACTTTCGGCCC19120.12129016705944298No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT18800.1192602061044732No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT18480.11723024514950346No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT18480.11723024514950346No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGGGCTTCTTCCCCCAGGAGCCACTCAG18350.11640557351154698No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCCTGGTCAAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC18160.11520028419453368No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC18050.11450248511626283No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC17950.11386812231783479No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17830.11310688695972114No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17780.1127897055605071No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCAGAGGAGACGGTGACCAGGGTTCCC17700.11228221532176468No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCG17210.10917383760946722No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATAGC17130.1086663473707248No Hit
GGGCTGGAGTGGGTCTCATCCATTAGTAGTAGTAGTAGTTACATATACTA16850.10689013153512626No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCACTGCCA16600.10530422453905613No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAATGGAGGCCAGTTGCTACGC16580.10517735197937053No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCG16560.10505047941968491No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA16510.10473329802047088No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC16500.10466986174062808No Hit
CCCCTACTTTCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCTGCATCCCCGACCAGCCCCAAGG16420.10416237150188563No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCTTAGCCCTGGAT16410.10409893522204283No Hit
GCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAG16300.10340113614377199No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGCA16250.10308395474455795No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGATGAGGAGCC16180.10263990078565834No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT16060.10187866542754466No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15990.10143461146864503No Hit
GCATAGTATATGTAACTACTACTACTACTAATGGATGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACC15960.10124430262911661No Hit
GTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGA15840.10048306727100295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG31300.088.8981570
GTTATTA651.3399238E-462.95276370
ACGATCA500.003744593661.3789470
TAGGTCG200.007538894751.4357541
TTGTACG200.00800522450.6607443
TGTTAGA10550.046.5432770
GGTATCA9600.042.5866621
CTTATAC4050.042.5475581
CTCTTAT1150.041.132931
AATGGTA2753.6379788E-1240.9192970
CGTATGC3950.040.80135341
TTATACA4250.040.5285952
ACGAGAC4100.039.56017322
GCCGTCT3950.039.28446
GCTAATC4000.039.0841433
TCGTATG4050.038.89462340
ATCTCGT3700.038.72604437
TGTACGG350.001496534938.5986634
CTAATCT3800.038.49248534
ATGCCGT4150.038.12908644
Produced by FastQC (version 0.11.5)