FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1216811
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG52260.4294832969129963No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATACCACATAAGTACCACTGTCTGCTGATTGA51930.42677128987163987No Hit
GTCTTTATATATCACCAACACAGGGGCCTGGCCTGGCTTTTGCTGGTACC49460.40647232807724454No Hit
ATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACGT46850.38502281784106157No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATACCACATAAGTACCACTGTCTGCTGATTGACAGTAATAG45870.37696897874854846No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG42620.3502598184927651No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC37810.3107302613142057No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG36810.30251205815858007No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGCATTGCCAAAG36720.3017724198745738No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC34370.2824596424588535No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGA32360.26594105411604596No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT31930.2624072267591269No Hit
GACTATTACTGTCAATCAGCAGACAGTGGTACTTATGTGGTATTTGGCGGAGGGACCAAGCTGACCGTCCTAGG31590.2596130376862142No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30330.2492581017101259No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG30010.24662827670032567No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA29610.2433409954380754No Hit
GTGTTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCG29240.24030026027049395No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28710.23594461259801233No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG28530.23446533602999972No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26590.21852202190808598No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26370.21671401721384834No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC26200.215316922677392No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGCATTGCCAAAGCAATATGCTTATTGG26060.21416637423560436No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25980.2135089179831543No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25130.20652344530087252No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25030.20570162498530997No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24250.19929142652392196No Hit
AAGTACCACTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA23430.19255249993630894No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22820.18753939601137726No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22780.18721066788515225No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22520.1850739350646896No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC22360.1837590225597895No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTG21970.18055392332909548No Hit
GAGTAGTATTGCTTCGCCAATCCAAAGTCGGGACCCCTCGCACAGTAATACACGGCCGTGTCCTCAGATCTCAGG21600.17751318816151399No Hit
CTATTACTGTCAATCAGCAGACAGTGGTACTTATGTGGTATTTGGCGGAG21400.17586954753038886No Hit
GCAATATGCTTATTGGTACCAGCAAAAGCCAGGCCAGGCCCCTGTGTTGGTGATATATAAAGACAGTGAGAGGCC21280.1748833631517138No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC20990.17250008423658236No Hit
CAGTGGTACTTATGTGGTATTTGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGG20840.1712673537632385No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA20760.17060989751078845No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATACCACATAAGTACCAC20510.16855534672188202No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGT20470.168226618595657No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19970.1641175170178442No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19940.16387097092317543No Hit
CATAAGTACCACTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACG19310.15869350293513126No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG19160.1574607724617874No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT19030.15639240605155608No Hit
GTGGTACTTATGTGGTATTTGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA18610.1529407607261933No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT18400.1512149380635119No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG17990.1478454747697054No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG17850.1466949263279178No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC17780.14611965210702402No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17580.1444760114758989No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATACCACATAAGTAC17400.14299673490788628No Hit
GTACCACTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA16980.1395450895825235No Hit
GGCCAGGCCCCTGTGTTGGTGATATATAAAGACAGTGAGAGGCCCTCAGG16900.13888763333007345No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16730.13749053879361708No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT16710.13732617473050457No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16650.13683308254116702No Hit
GTGTATTACTGTGCGAGGGGTCCCGACTTTGGATTGGCGAAGCAATACTA16640.13675090050961078No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16400.13477853175226062No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16370.13453198565759183No Hit
ATACTACTCCATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGG16170.13288834502646674No Hit
GCTTGGTCCCTCCGCCAAATACCACATAAGTACCACTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTTC15820.13001197392199776No Hit
CAGTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTA15800.12984760985888524No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15690.12894360751176642No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15050.123683957492166No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14940.12277995514504718No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCAGACAGTGGTACTTATGTGGTATT14890.1223690449872659No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14880.12228686295570963No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14620.12015013013524697No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCT14540.11949267388279691No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG14540.11949267388279691No Hit
GGATCACCTGCTCTGCAGATGCATTGCCAAAGCAATATGCTTATTGGTACCAGCAAAAGCCAGGCCAGGCCC14330.11776685122011554No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14320.11768466918855927No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14300.11752030512544676No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14230.11694503090455297No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14160.11636975668365918No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14160.11636975668365918No Hit
GTATTACTGTGCGAGGGGTCCCGACTTTGGATTGGCGAAGCAATACTACTCCATGGACGTCTGGGGCAAAGGGAC14100.11587666449432163No Hit
CCCCTGTGTTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCT13960.11472611605253404No Hit
ACACAGGGGCCTGGCCTGGCTTTTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTGCAGAGCAGGTGA13940.11456175198942153No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13930.11447956995786528No Hit
GTATTTGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC13910.11431520589475276No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCG13810.1134933855791902No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG13510.11102792463250251No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGGGGTCCCGACTTTGGA13440.11045265041160869No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13360.10979519415915866No Hit
CCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCTCTCA13330.10954864806448988No Hit
CAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCAGACAGTGGTA13270.10905555587515234No Hit
GCATATTGCTTTGGCAATGCATCTGCAGAGCAGGTGATCCTGGCCGTCTGTCCTGGGGACACTGACACCGAGGG13090.10757627930713973No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12820.10535736445512081No Hit
GTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCAGAC12820.10535736445512081No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC12710.10445336210800199No Hit
CACCAACACAGGGGCCTGGCCTGGCTTTTGCTGGTACCAATAAGCATATT12640.10387808788710819No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC12630.10379590585555193No Hit
GTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACGTTGACCATCAG12600.10354935976088316No Hit
ACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCTCTCA12550.10313844960310188No Hit
GGGATCCCTGAGGGCCTCTCA12520.10289190350843312No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGC12430.10215226522442682No Hit
GTCCTGGGGACACTGACACCGAGGGTGGCTGTGTCAGCTCATAGGAGGCC12410.1019879011613143No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG12310.10116608084575172No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT12260.10075517068797045No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGCATTGCCAAAGCAATATGCT12230.10050862459330166No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA12180.10009771443552039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2600.066.1819370
GTCTTTA5600.058.6533161
TCTTTAT6500.051.0257642
ACGCAGC200.00793715950.7696046
CTTTATA6550.050.6383363
TCTGGTA650.00867329249.63644870
CGCGGTA12400.049.436370
TTTATAT6650.049.369944
TGGTATC2200.049.227082
TATTCTA1102.3824603E-548.8843870
TTATATA6800.048.280895
TATATAT7350.045.1285326
TATACAC2550.045.1266823
TCGTATG2550.044.36829440
CAATGCA22700.043.58677770
TATATCA7650.043.357018
GGTATCA7200.043.267741
CGTATGC2650.042.7402841
TATCTCG2400.042.6816236
TTATACA2700.042.617892
Produced by FastQC (version 0.11.5)