FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546090
Sequences flagged as poor quality0
Sequence length18-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC14290.26167847790657217No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAA12150.22249079822007362No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG11240.20582687835338495No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT11040.2021644783826842No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTT9960.18238751854089985No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTT9830.18000695855994434No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCG9080.16627295866981634No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT8690.15913127872694977No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGA8550.1565675987474592No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA8520.15601823875185408No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC8110.14851031881191745No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT8030.14704535882363715No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGG7840.14356607885147138No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC7400.13550879891592962No Hit
GAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTT6710.12287351901701185No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC6680.12232415902140673No Hit
CCTTTAATAGCGGCTGCACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGAC6670.12214103902287168No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCCAACCTCCGAGCAGTA6630.12140855902873153No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTGCCGAGTTCCTTTTACTT6570.1203098390375213No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGA6420.1175630390594957No Hit
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGT6290.11518247907854016No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAG6040.11060447911516416No Hit
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGC6030.11042135911662912No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGTGCAGC5890.10785767913713856No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCT5750.10529399915764802No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA5570.1019978391840173No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT5500.10071599919427202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2950.071.0783870
GTTAAGC252.077544E-457.60923855
AACGTAA600.00847031349.92409570
TACTTAC200.00863147549.7000351
GTCTATA200.00863147549.7000351
CTCGTAT1950.049.36665339
TCGTATG2150.048.1372740
GACCGGA3600.044.37697670
ACGAGAC2250.044.2183122
TCTCGTA2200.043.5869638
CGTATGC2450.042.36006541
TATACAC2600.040.7683143
TTATACA2650.039.9991042
TAGGCAA2251.2278178E-939.93927470
ATCTCGT2400.039.81149737
ACGCGTA2550.039.1958227
GACGCGT2550.039.12735426
AGACGCG2550.039.098625
GTATCAA34550.038.7434961
TAATCTC2450.038.732335
Produced by FastQC (version 0.11.5)