FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951446
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT42540.4471089268334724No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42120.44269459328222516No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTGTTATTGTTGGAGGTGTAGAAAACAC34600.36365700207894086No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCACCGGGTC34450.3620804543820669No Hit
GTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAA33340.35041400142519913No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT28350.29796751470919003No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26510.27862852962753537No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25620.2692743466260828No Hit
ATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCA25580.2688539339069164No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA24770.2603405763437967No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24490.25739768730963186No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24440.25687217141067387No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24180.2541394887360922No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23900.25119659970192737No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC23800.2501455679040114No Hit
ATTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCT23100.24278834531859927No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22920.24089648808235045No Hit
TTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCT22880.24047607536318402No Hit
GAGTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAA22090.23217292415964752No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21940.2305963764627735No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21240.2232391538773614No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGGTGCCA20680.21735337580903172No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT20090.21115228820132725No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC19090.20064197022216712No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGAGACATCGTGATGACCCAG17580.1847713900736353No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17440.18329994555655288No Hit
GAAATATACAGCCGTGTCCTCGGCTCTCAGACTATTCATTTGCAGATACA16390.17226411167843472No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTCTA15930.16742936540802106No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15820.16627323043031345No Hit
GTTTATTACTGTCAGCAATATTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA15810.16616812725052185No Hit
GTCCCAGACCCGGTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT15780.16585281771114702No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAG15750.16553750817177224No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15650.16448647637385622No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15600.1639609604748982No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTCTACACCTCCAA15480.162699722317399No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC15420.16206910323864937No Hit
GGCCAAAAGTGTACGGAGTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGA15320.16101807144073338No Hit
GAGTGAAATCTGTCCCAGACCCGGTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAA14730.1548169838330289No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAATAC14700.15450167429365408No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14580.15324043613615485No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14530.15271492023719685No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14490.15229450751803045No Hit
GTCCCTGACCGATTCAGTGGCACCGGGTCTGGGACAGATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGA14420.15155878525948924No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGGTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGA14360.15092816618073962No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14090.1480903803263664No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14050.1476699676072No Hit
GTTTTCTACACCTCCAACAATAACAACTACTTAGCTTGGTACCAGCAGAA14020.1473546580678252No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13880.14588321355074277No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13700.14399135631449395No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCACCGGGTCTGGGACAGATT13690.14388625313470235No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13690.14388625313470235No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13430.1411535704601207No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA13270.1394719195834551No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13170.13842088778553907No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA13010.13673923690887343No Hit
TCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGGTGCCACTGAATCGGTCAGGGACC12910.13568820511095742No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTGTTATTGTTGGAGGTG12900.13558310193116582No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12740.13390145105450021No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTCTACACCTCCAACAATAACAACTA12460.13095856202033535No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGGTGCCA12260.12885649842450334No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT12150.12770036344679572No Hit
ATATTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA12140.12759526026700413No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11800.12402175215408967No Hit
CACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAATACTCCGTACACTTT11600.12191968855825763No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11550.12139417265929964No Hit
ATTCAGTGGCACCGGGTCTGGGACAGATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTA11520.12107886311992483No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGG11410.11992272814221722No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11380.11960741860284241No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG11370.1195023154230508No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11350.1192921090634676No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11340.11918700588367602No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11280.11855638680492639No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA11260.11834618044534319No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATC11200.11771556136659359No Hit
GGATCTCTGGTGCCTACGGAGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG10900.11456246597284556No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10720.11267060873659672No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT10690.11235529919722191No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10600.1114093705790975No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10570.1110940610397227No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG10560.1109889578599311No Hit
CTACTACTCTCATCTCTCACACAGAAATATACAGCCGTGTCCTCGGCTCT10470.11004302924180669No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10210.10731034656722505No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10120.10636441794910063No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10010.10520828297139301No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT9980.10489297343201823No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9970.10478787025222662No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9860.103631735274519No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9810.103106219375561No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTT9800.10300111619576939No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC9780.10279090983618619No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9660.10152967167868696No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT9620.10110925895952057No Hit
GAGTAGTAGTTGGCGGGGGTACTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGGAGTGCATC9580.10068884624035415No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9560.10047863988077094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCGT453.24344E-853.20012337
ACGCCCG200.00695115352.50085457
AGACGAC200.00788744750.84950320
GATGGAG7000.048.5262970
TGTTAGA10300.048.49857770
TGGTATC1600.046.57532
AATGGTA1953.0195224E-1046.11095470
GGTATCA6150.045.7243161
AGCGATG606.2318577E-945.4553833
TCCGAGA8800.043.1416970
TCGTATG2000.042.8729540
CTTATAC2400.042.3500861
CACGAGA2350.041.83803621
CGTATGC2150.041.51022341
GTGGTAT1800.041.4089661
ATGCCGT2250.041.33179544
GTATCAA16850.041.219071
TATACAC2550.041.1810383
GTCGTAT504.0441028E-641.09242639
GCGTAGA25450.040.43390370
Produced by FastQC (version 0.11.5)