FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004685865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951446
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41790.4392261883491023No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA39460.4147371474576592No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCACCGGGTCTG34820.36596927203435614No Hit
GTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAAT33840.3556691604147792No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29240.30732169771064255No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTGTTATTGTTGGAGGTGTAGAAAACACT28740.3020665387210625No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT27110.28493472041503143No Hit
ATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA25600.2690641402664996No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC24560.25813340956817304No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24150.2538241791967174No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA23510.24709757569005494No Hit
ATTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA23140.24320875803776568No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGGTGAGGAAGATGCTGGCAAAGGATGG22200.23332905913735513No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21810.2292300351254827No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21550.22649735245090105No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT21540.22639224927110943No Hit
TTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT21320.22407997931569423No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC20920.21987585212403016No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC20700.21756358216861493No Hit
GAGTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAAT20600.21651255037069891No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGGTGCCA19800.2081042959873708No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA19510.20505630377341436No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19420.20411037515528996No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19140.2011674861211251No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17840.18750407274821693No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA17650.18550711233217648No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17320.18203870739905365No Hit
GTTTATTACTGTCAGCAATATTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA16520.17363045301572555No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT16520.17363045301572555No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTCTA16360.17194880213905991No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA15920.16732426222822946No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTCTACACCTCCAA15490.1628048254971906No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15440.16227930959823259No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15240.16017724600240058No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15210.15986193646302577No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14880.1563935315299029No Hit
GTCCCAGACCCGGTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTA14840.1559731188107365No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTC14350.15082306300094803No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC14340.15071795982115643No Hit
GGCCAAAAGTGTACGGAGTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTT14290.15019244392219844No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGA14050.1476699676072No Hit
GAAATATACAGCCGTGTCCTCGGCTCTCAGACTATTCATTTGCAGATACAGCGTGTTCTTGGCGTTGTCTCTGGA14000.14714445170824197No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13930.14640872944970076No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTG13930.14640872944970076No Hit
GAGTGAAATCTGTCCCAGACCCGGTGCCACTGAATCGGTCAGGGACCCCG13470.1415739831792871No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13470.1415739831792871No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGGTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTA13460.1414688799994955No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13190.13863109414512229No Hit
GTCCCTGACCGATTCAGTGGCACCGGGTCTGGGACAGATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGAT13160.13831578460574745No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCACCGGGTCTGGGACAGATTT13030.13694944326845665No Hit
GTTTTCTACACCTCCAACAATAACAACTACTTAGCTTGGTACCAGCAGAA12720.133691244694917No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12410.13043304612137735No Hit
ATATTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT12310.12938201432346133No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAATACT12270.12896160160429496No Hit
CACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC12140.12759526026700413No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12030.1264391252892965No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTGTTATTGTTGGAGGTG12000.1261238157499217No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT11960.12570340303075528No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11910.12517788713179728No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11860.12465237123283927No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTGTTATTGTTGGAGGTGTA11770.12370644261471486No Hit
TCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGGTGCCACTGAATCGGTCAGGGACC11660.12255030763700725No Hit
ATTCAGTGGCACCGGGTCTGGGACAGATTTCACTCTCACCATCAACAGCC11610.12202479173804924No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTCTACACCTCCAACAATAACAACTA11500.12086865676034164No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG11340.11918700588367602No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11280.11855638680492639No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC11250.11824107726555161No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGGTGCCA11220.11792576772617679No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11210.11782066454638518No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11210.11782066454638518No Hit
GGATCTCTGGTGCCTACGGAGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGG11210.11782066454638518No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11050.11613901366971957No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGTATT10860.11414205325367913No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA10640.11182978329826392No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGA10570.1110940610397227No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATC10560.1109889578599311No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA10510.11046344196097307No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA10340.10867668790451586No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10310.10836137836514106No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10140.10657462430868385No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9990.1049980766118098No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9870.1037368384543106No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC9680.10173987803827017No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9670.10163477485847856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)