FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514415
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGA74450.4916089711208618No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTA73750.48698672424665634No Hit
CCTCTGAAGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT55110.36390289319638275No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG50810.3355090909691201No Hit
GTCCTGGGCCCAGTCTGTACTGACTCAGTCACCCTCAGCGTCTGGGACCC45600.3011063678053902No Hit
CTTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCACCTTGGTCCCTC44170.2916637777623703No Hit
GTACTGACTCAGTCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC42190.2785894223181889No Hit
AGTCTGTACTGACTCAGTCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG38510.25428961017950824No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT37500.24762036826101166No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAAGAGC37030.24451685964547365No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAAGAGCTTCAAGCCAACAAGG36750.24266796089579146No Hit
GACGTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCACACCATTCAGG35880.23692316835213595No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGTCACCCTCAGCGTCT35870.23685713625393304No Hit
GCCTTGGGCTGACGTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCACACCATTCAGGCTGTCATCCCATG34250.22615993634505735No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33340.2201510154085901No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG32500.21460431915954348No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCTTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG32470.21440622286493463No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG31920.21077445746377316No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31590.2085953982230762No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCTTCAGAGGA31400.20734078835722042No Hit
CCCCTGAGGGCCGCTGATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGAACCAGCTTA30810.2034448945632472No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA30740.20298266987582664No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC30450.20106773902794148No Hit
CTCTGTTCCCGCCCTCCTCTGAAGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTATC30220.19954900076927395No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28870.19063466751187752No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA28440.18779528728915126No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG27580.18211652684369872No Hit
GATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG27580.18211652684369872No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT26750.17663586269285497No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGTCAC26700.1763057022018403No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25950.17135329483662007No Hit
GCATGGGATGACAGCCTGAATGGTGTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTACGTCAGCCCAAGG25930.1712212306402142No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG25560.16877804300670557No Hit
CTCTTGTTCTGGGAGCAGCTCCAACATCGGAAGTAATGATGTAAGCTGGTTCCAGCAGCTCCCAGGAACGGCCCC25370.16752343314084978No Hit
GTTCCCGCCCTCCTCTGAAGAGCTTCAAGCCAACAAGGCCACACTGGTGT24500.1617786405971943No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGACTGAGGTGCCAGACCTGGAGCCAGAGAATCGCTCAGGGACC24460.16151451220438254No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTATCCGGGAGCCGTGACAGTGGC24450.16144848010617963No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGATAGAAGTCACTTA24170.15959958135649738No Hit
CCTCAGGGGTCCCTGAGCGATTCTCTGGCTCCAGGTCTGGCACCTCAGTCTCCCTGGCCATCAGTGGGCTCCAGT23990.15841100358884452No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTGTTCGGCGGAGGGAC23950.1581468751960328No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA23170.15299637153620374No Hit
TTCCAGGCCACTGTCACGGCTCCCGGATAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG22080.14579887283208368No Hit
GTGTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC21510.1420350432345163No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21170.13978995189561647No Hit
ACACCACACCATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC20820.13747882845851367No Hit
GGTGTGTCTCATAAGTGACTTCTATCCGGGAGCCGTGACAGTGGCCTGGA20820.13747882845851367No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCACAC20300.13404515935196099No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCACCTTGGTC20270.13384706305735217No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGAGCGATTCTCTGGCTCCAGG20100.13272451738790225No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19930.13160197171845234No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT19880.13127181122743764No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGAACCAGCTTACATCATTACTT19800.13074355444181418No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19650.12975307296877012No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC19630.12962100877236424No Hit
CCATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTC19290.1273759174334644No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCAC19240.1270457569424497No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19220.12691369274604383No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGATAGAAGTCACTTATGAGACACACCAGTGTGGC19210.12684766064784092No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19180.1266495643532321No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC19170.12658353225502916No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA18920.12493272979995575No Hit
CTGATGGCCAGGGAGACTGAGGTGCCAGACCTGGAGCCAGAGAATCGCTC18790.12407431252331759No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT18700.12348002363949115No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG18510.12222541377363537No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTATCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC18400.12149906069340306No Hit
GAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGCTGAGGGTGA18290.12077270761317077No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG18250.12050857922035901No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC18250.12050857922035901No Hit
CCTTGGTCCCTCCGCCGAACACCACACCATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCT18250.12050857922035901No Hit
ACGTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCACACCATTCAGGC18230.12037651502395313No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC18170.11998032243473553No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC17730.11707491011380632No Hit
GTGCAGCATGGGATGACAGCCTGAATGGTGTGGTGTTCGGCGGAGGGACC17700.11687681381919751No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCTTCAGAGGAGGGCGGGAACAGAGTG17700.11687681381919751No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA17670.11667871752458871No Hit
GGACCAAGGTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAAGAG17080.11278282373061545No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAAGAGCTTCAAGCCAACAAGGCC16670.11007550770429506No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16670.11007550770429506No Hit
CTGTCACGGCTCCCGGATAGAAGTCACTTATGAGACACACCAGTGTGGCC16630.10981137931148331No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16280.10750025587438054No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGACTGAGGTGCCAGAC15840.10459484355345133No Hit
CAGTAATACAAGGCCGTGTCCTCGGCTCTCAGATTGTTCACTTGCAGATA15780.10419865096423372No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15670.1034722978840014No Hit
GTCACGGCTCCCGGATAGAAGTCACTTATGAGACACACCAGTGTGGCCTT15610.1030761052947838No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT15520.10248181641095737No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCTTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15510.10241578431275443No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15510.10241578431275443No Hit
GTATTACTGTGCGAGAGTTCATAGCAGTGGTTATTACTTCCCTTTTGACTACTGGGGCCAGGGAACCCTGGTCAC15480.10221768801814562No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAT200.007721203451.1257321
TCGTATG2150.048.09722540
CGTATGC2250.046.0302841
ATCTCGT2300.044.795937
TATCTCG2300.043.2681536
GTGGTAT3800.043.053251
GGTATCA12050.042.1451451
GCCTATC2250.041.06738333
TCTCGTA2400.040.1088138
GTATAGA350.001430809338.9529381
ACGAGAC2750.038.4255722
AATGGTA4600.038.4150770
CCTATCT2500.038.3486334
TGGTATC4200.038.141422
CCGTCTT2400.037.7047847
GCCGTCT2400.037.65526646
CACGAGA2900.037.61097721
ATGCCGT2500.037.44608744
CCCACGA3100.037.37855519
GTATGCC2800.037.0431342
Produced by FastQC (version 0.11.5)