FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514415
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTA66080.436340104925004No Hit
GTCCTGGGCCCAGTCTGTACTGACTCAGTCACCCTCAGCGTCTGGGACCC53950.3562431698048421No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG51150.33775418230801996No Hit
CCTCTGAAGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT49300.32553824414047666No Hit
GTACTGACTCAGTCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC46730.308567994902322No Hit
AGTCTGTACTGACTCAGTCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG42100.27799513343436244No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGTCACCCTCAGCGTCT41200.27205224459609817No Hit
CTTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCACCTTGGTCCCTC38730.25574231633997285No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG34220.2259618400504485No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32400.21394399817751408No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGA32370.2137459018829053No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32080.2118309710350201No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31930.21084048956197607No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAAGAGC31690.2092557192051056No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAAGAGCTTCAAGCCAACAAGG31650.20899159081229388No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGTCAC29710.1961813637609242No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC29350.19380420822561847No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG29310.19354007983280674No Hit
CTCTGTTCCCGCCCTCCTCTGAAGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTATC28780.1900403786280511No Hit
CCCCTGAGGGCCGCTGATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGAACCAGCTTA28420.18766322309274538No Hit
GCCTTGGGCTGACGTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCACACCATTCAGGCTGTCATCCCATG28340.18713496630712187No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27910.18429558608439564No Hit
CTCTTGTTCTGGGAGCAGCTCCAACATCGGAAGTAATGATGTAAGCTGGTTCCAGCAGCTCCCAGGAACGGCCCC27700.18290891202213394No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27420.18106001327245175No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCTTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27310.18033366019221944No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCTTCAGAGGA27060.178682857737146No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26280.17353235407731699No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG26190.17293806519349056No Hit
GACGTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCACACCATTCAGG26090.1722777442114612No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25780.17023074916717015No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG25600.1690421713995173No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25400.16772152943545857No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTGTTCGGCGGAGGGAC25110.1658065985875734No Hit
GCATGGGATGACAGCCTGAATGGTGTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTACGTCAGCCCAAGG25020.165212309703747No Hit
CCTCAGGGGTCCCTGAGCGATTCTCTGGCTCCAGGTCTGGCACCTCAGTCTCCCTGGCCATCAGTGGGCTCCAGT24570.16224086528461484No Hit
GATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG24510.16184467269539723No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA24410.16118435171336787No Hit
GTGTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC24230.15999577394571501No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23580.15570368756252415No Hit
GTTCCCGCCCTCCTCTGAAGAGCTTCAAGCCAACAAGGCCACACTGGTGT23180.15306240363440668No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT23000.15187382586675383No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22590.14916650984043342No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGAGCGATTCTCTGGCTCCAGG21920.1447423592608367No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTATCCGGGAGCCGTGACAGTGGC21110.13939375930639883No Hit
GGTGTGTCTCATAAGTGACTTCTATCCGGGAGCCGTGACAGTGGCCTGGA20960.1384032778333548No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGATAGAAGTCACTTATGAGACACACCAGTGTGGC20910.13807311734234012No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20770.137148667967499No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGATAGAAGTCACTTA20700.13668644328007845No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG20400.13470548033399035No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC19950.13173403591485822No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA19720.13021529765619066No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19680.12995116926337894No Hit
TTCCAGGCCACTGTCACGGCTCCCGGATAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19290.1273759174334644No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC19210.12684766064784092No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGAACCAGCTTACATCATTACTT18670.12328192734488234No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC18620.12295176685386768No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18500.12215938167543243No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG18060.11925396935450322No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCACAC17710.11694284591740045No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17670.11667871752458871No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT17520.11568823605154466No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT17500.11555617185513879No Hit
CTGATGGCCAGGGAGACTGAGGTGCCAGACCTGGAGCCAGAGAATCGCTC17400.11489585087310943No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA17170.11337711261444189No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16770.11073582868632442No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC16580.10948121882046863No Hit
GTGCAGCATGGGATGACAGCCTGAATGGTGTGGTGTTCGGCGGAGGGACC16570.1094151867222657No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT16530.10915105832945393No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16420.10842470524922165No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC16370.10809454475820696No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCTTCAGAGGAGGGCGGGAACAGAGTG16350.10796248056180108No Hit
GTCTGTACTGACTCAGTCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGGAGCAG16330.10783041636539521No Hit
GGGTCACCATCTCTTGTTCTGGGAGCAGCTCCAACATCGGAAGTAATGAT16010.10571738922290125No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAAGAGCTTCAAGCCAACAAGGCC15950.10532119663368364No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCACCTTGGTC15860.1047269077498572No Hit
ACACCACACCATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC15560.1027459448037691No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTATCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15470.10215165591994269No Hit
GGACCAAGGTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAAGAG15300.10102911025049277No Hit
GAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGCTGAGGGTGA15180.10023672507205753No Hit
GCCCTCAGGGGTCCCTGAGCGATTCTCTGGCTCCAGGTCTGGCACCTCAG15160.10010466087565166No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)