FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004686065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286833
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATATAGCTCCAATAACTTGAACTATTTAGCTTGGTACCAGCAGAG71770.5577258276715005No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT56910.44224852797526953No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56840.4417045568461487No Hit
CTCCTAAGCTGATCATTTACTGGGCATCTACCCGGGAGTCCGGGGTCCCT55780.4334672797480326No Hit
ATATAAAACACTCCGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA54020.4197902913587077No Hit
GCTTAGGAGGCTGCCCTGGTCTCTGCTGGTACCAAGCTAAATAGTTCAAG50740.39430135845132974No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG48870.37976955828767217No Hit
ATATTATAGTACGCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT48830.3794587176424602No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46910.3645383666722877No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCAC36580.2842637700463075No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36520.2837975090784896No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34280.2663904329466217No Hit
GTACCAGCAGAGACCAGGGCAGCCTCCTAAGCTGATCATTTACTGGGCATCTACCCGGGAGTCCGGGGTCCCTGA34180.2656133313335919No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32240.25053756004081335No Hit
GACCAGGGCAGCCTCCTAAGCTGATCATTTACTGGGCATCTACCCGGGAG31270.24299967439442416No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31140.24198944229748537No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT29050.2257480185851622No Hit
ACCCGGGAGTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCACCATCAGCAGC28280.2197643361648326No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28110.21844326342268186No Hit
GTTTATTACTGTCAGCAATATTATAGTACGCCGACGTTCGGCCAAGGGAC27830.2162673789061984No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27300.21214874035714038No Hit
ATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCACCATCAGCAGCC27010.20989514567935386No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG27000.2098174355180509No Hit
CTGCTGGTACCAAGCTAAATAGTTCAAGTTATTGGAGCTATATAAAACAC26830.20849636277590022No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26690.20740842051765848No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26480.20577650713029583No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGT26150.20321207180729745No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25920.2014247380973289No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25840.20080305680690502No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25820.20064763648429906No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC25620.19909343325823942No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25480.19800549099999765No Hit
GTCAGGGACCCCGGACTCCCGGGTAGATGCCCAGTAAATGATCAGCTTAGGAGGCTGCCCTGGTCTCTGCTGGTA24570.19093386632142634No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23930.18596041599803548No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22450.1744593121251942No Hit
GCAGAGACCAGGGCAGCCTCCTAAGCTGATCATTTACTGGGCATCTACCCGGGAGTCCGGGGTCCCTGACCGATT21970.17072922438265103No Hit
ATTTAGCTTGGTACCAGCAGAGACCAGGGCAGCCTCCTAAGCTGATCATT21960.17065151422134806No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA21900.17018525325353018No Hit
GATCATTTACTGGGCATCTACCCGGGAGTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGAGACAGATTT21830.16964128212440932No Hit
GGTCTCTGCTGGTACCAAGCTAAATAGTTCAAGTTATTGGAGCTATATAA21240.1650563826075334No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21130.16420157083320058No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA20990.16311362857495884No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20880.16225881680062604No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTGTTT20740.16117087454238427No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCTCAGACCCGCTGCCA20700.16086003389717238No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC20340.158062468090265No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20040.15573116325117556No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACG19270.14974748083084596No Hit
GCCCTGGTCTCTGCTGGTACCAAGCTAAATAGTTCAAGTTATTGGAGCTA19180.14904808937911912No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACGCCGACGTTCGG19140.1487372487339072No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18570.14430776953963723No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18540.1440746390557283No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18420.1431421171200925No Hit
GTAAATGATCAGCTTAGGAGGCTGCCCTGGTCTCTGCTGGTACCAAGCTA18320.1423650155070627No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18300.14220959518445672No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT17870.1388680582484285No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17590.136692173731945No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17140.13319521647331084No Hit
GTCTGAGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG17110.1329620859894019No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16540.12853260679513193No Hit
GTCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAA16520.12837718647252597No Hit
GGATCTCTGGTGTCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG16470.12798863566601107No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15770.12254892437480233No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15620.1213832719552576No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15510.12052846018092479No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACGCCGA15490.12037303985831882No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15450.12006219921310692No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG15380.11951822808398604No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15190.11804173501922938No Hit
CTCCCGGGTAGATGCCCAGTAAATGATCAGCTTAGGAGGCTGCCCTGGTCTCTGCTGGTACCAAGCTAAATAGTT14950.11617669114795781No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14690.11415622695408029No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14640.11376767614756537No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA14610.11353454566365644No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14540.11299057453453556No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT14330.11135866114717294No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14270.11089240017935506No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14180.11019300872762822No Hit
GTCCTGTATAGCCTCTGGATTCAGATTCACCTTCAGTGACCACTACATGA13910.1080948343724477No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13890.10793941404984174No Hit
GCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGCGT13880.10786170388853876No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13830.10747315308202385No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13730.10669605146899402No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13730.10669605146899402No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13650.10607437017857019No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13500.10490871775902545No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13370.10389848566208669No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC13310.1034322246942688No Hit
GTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCACCATCAGCAGCCTGCAGG13230.10281054340384493No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13060.10148947066169425No Hit
CTACCCGGGAGTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGAG13050.10141176050039127No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13050.10141176050039127No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13020.10117863001648233No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCTCAGACCCGCTGCCA12950.10063465888736145No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12920.10040152840345251No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)