FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286833
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT72940.5668179165439493No Hit
ATATTATAGTACGCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT68360.5312266626671838No Hit
GTTTTATATAGCTCCAATAACTTGAACTATTTAGCTTGGTACCAGCAGAG59130.45950018378453145No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51510.40028504087165934No Hit
GCTTAGGAGGCTGCCCTGGTCTCTGCTGGTACCAAGCTAAATAGTTCAAG43520.33819462199057687No Hit
CTCCTAAGCTGATCATTTACTGGGCATCTACCCGGGAGTCCGGGGTCCCT43180.3355524765062755No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40490.3146484431157734No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36700.2851962919819433No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35320.2744722897221318No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33850.26304889601059345No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCTCAGACCCGCTGCCA33710.26196095375235173No Hit
GTTTATTACTGTCAGCAATATTATAGTACGCCGACGTTCGGCCAAGGGAC30400.2362388903610647No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29270.22745764213382777No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCAC28920.2247377864882234No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28780.22364984422998166No Hit
ATATAAAACACTCCGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA28690.22295045277825484No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGT28140.21867639390659083No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26880.20888491358241512No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24910.1935760118057277No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24670.19171096793445613No Hit
GACCAGGGCAGCCTCCTAAGCTGATCATTTACTGGGCATCTACCCGGGAG23710.18425079244936987No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23710.18425079244936987No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT23710.18425079244936987No Hit
GTACCAGCAGAGACCAGGGCAGCCTCCTAAGCTGATCATTTACTGGGCATCTACCCGGGAGTCCGGGGTCCCTGA23560.18308514002982518No Hit
ATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCACCATCAGCAGCC23330.18129780631985656No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22640.17593580518995083No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22610.1757026747060419No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG22470.17461473244780015No Hit
CTGCTGGTACCAAGCTAAATAGTTCAAGTTATTGGAGCTATATAAAACAC22330.1735267901895584No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22120.17189487680219578No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22110.1718171666408928No Hit
ACCCGGGAGTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCACCATCAGCAGC21470.16684371631750194No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCTCAGACCCGCTGCCACTGAATCGGTCAGGGACC21330.1657557740592602No Hit
GTCAGGGACCCCGGACTCCCGGGTAGATGCCCAGTAAATGATCAGCTTAGGAGGCTGCCCTGGTCTCTGCTGGTA21250.16513409276883637No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21200.16474554196232147No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21140.16427928099450356No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC21000.16319133873626182No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20550.15969438147762763No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20150.15658597502550836No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19830.15409924986381293No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCTCAGACCCGCTGCCA19660.15277817712166225No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19210.14928121986302806No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19120.14858182841130121No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18980.1474938861530595No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18710.14539571179787897No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG18520.14391921873312233No Hit
GGTCTCTGCTGGTACCAAGCTAAATAGTTCAAGTTATTGGAGCTATATAA18330.1424427256683657No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACG18230.14166562405533586No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18150.141043942764912No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACGCCGACGTTCGG18100.14065539195839707No Hit
GATCATTTACTGGGCATCTACCCGGGAGTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGAGACAGATTT17680.13739156518367185No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACGCCGA17610.136847594054551No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT17510.13607049244152117No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA16990.13202956405376612No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16960.13179643356985715No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16960.13179643356985715No Hit
GTCTCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGACTCCCGGGT16910.13140788276334225No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTGTTT16870.13109704211813034No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16570.12876573727904086No Hit
GCAGAGACCAGGGCAGCCTCCTAAGCTGATCATTTACTGGGCATCTACCCGGGAGTCCGGGGTCCCTGACCGATT16440.1277555051821021No Hit
GTCTGAGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG16390.1273669543755872No Hit
GAGTGAAATCTGTCTCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG16220.1260458816334365No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC16030.12456938856867986No Hit
GGATCTCTGGTGTCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG15810.12285976502001425No Hit
GCCCTGGTCTCTGCTGGTACCAAGCTAAATAGTTCAAGTTATTGGAGCTA15730.1222380837295904No Hit
ATTTAGCTTGGTACCAGCAGAGACCAGGGCAGCCTCCTAAGCTGATCATT15520.12060617034222777No Hit
GTCCTGTATAGCCTCTGGATTCAGATTCACCTTCAGTGACCACTACATGA15490.12037303985831882No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT15090.11726463340619955No Hit
GTAAATGATCAGCTTAGGAGGCTGCCCTGGTCTCTGCTGGTACCAAGCTA15050.11695379276098763No Hit
GTCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAA14980.11640982163186675No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14850.115399589534928No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA14770.11477790824450415No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14310.11120324082456699No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGCGTACTATAATATTGCTGACAGT14290.11104782050196102No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14240.11065926969544612No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14040.10910506646938647No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13930.10825025469505367No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13820.10739544292072087No Hit
CCTTGGTCCCTTGGCCGAACGTCGGCGTACTATAATATTGCTGACAGTAA13530.1051418482429344No Hit
CTCCCGGGTAGATGCCCAGTAAATGATCAGCTTAGGAGGCTGCCCTGGTCTCTGCTGGTACCAAGCTAAATAGTT13520.10506413808163143No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13500.10490871775902545No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13460.10459787711381352No Hit
GCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGCGT13330.10358764501687476No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13280.10319909421035985No Hit
GCTGATGGTGAGAGTGAAATCTGTCTCAGACCCGCTGCCACTGAATCGGT13090.1017226011456032No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13020.10117863001648233No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGTCTACGGGGACATCGTGATGA12930.10047923856475549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3350.045.754271
GGTATCA13400.044.2291261
TGGTATC3750.039.0465662
GTATCAA33450.036.8617821
CGCGCGT400.002694574934.23996432
TAGCGCG400.002720501534.17315729
GGTCTAG400.00276450334.0615121
AATGGTA3500.033.93163370
TATCAAC36900.033.22172
ATCAACG39000.031.5988623
TCAACGC39050.031.4626644
CAACGCA39800.030.9552865
AACGCAG39950.030.5834886
GACAGGA1900.030.476091
AGGGAAG9650.029.68113170
CGCAGAG41650.029.4168958
AGAGTAC41800.029.22650311
CAGAGTA42300.028.9626110
TTGCAAT1454.493185E-528.90727470
GCAGAGT42750.028.5803599
Produced by FastQC (version 0.11.5)