FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146864
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43300.37755130512423446No Hit
GAGTTACACTACCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT42080.3669136009151914No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41360.36063561154592No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30680.2675121025683952No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAATTCTGTC29930.26097253030873757No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCA29490.2571359812497384No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29030.2531250435971484No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28870.25172993484842143No Hit
GAATTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACTACC27060.23594776712844767No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26940.23490143556690246No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26810.2337679097085618No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26050.2271411431521087No Hit
ACCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG25980.22653078307454066No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAGAAA24530.21388761003920254No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24260.21153336402572578No Hit
GGGTAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAATT22930.19993652255193292No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22650.19749508224166076No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG22230.19383292177625244No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22050.19226342443393463No Hit
GCATAGATCAGGACTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT22040.1921762301371392No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21610.18842687537493547No Hit
GTCCTGATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT21360.1862470179550496No Hit
ACACTACCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACT21320.18589824076786785No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21160.1845031320191409No Hit
GATCAGGACTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT21040.1834568004575957No Hit
GGTGAGGGTAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAG20870.18197449741207328No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20420.17805075405627868No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20130.17552211944921106No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA19950.17395262210689325No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19900.17351665062291605No Hit
CTGCTACTCTGGCTCCGGGGTGCCAGAGGTGACATCCAGATGACCCAGTC19840.17299348484214344No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGGGGTGCCAGAGGTGACAT19280.16811060422159907No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18820.16409966656900907No Hit
ACTTACTACTGTCAACAGAGTTACACTACCCTCACCTTCGGCCAAGGGAC18150.1582576486837149No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC17450.1521540479080344No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16540.14421936689964981No Hit
CCCTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG16400.14299864674451374No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16260.14177792658937766No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC16240.14160353799578676No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCA16120.14055720643424155No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15660.13654626878165155No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15340.1337560512841976No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15170.13227374823867522No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14780.12887317066365322No Hit
GTCTCCCTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAC14620.12747806191492628No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA14460.1260829531661993No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14340.1250366216046541No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14300.12468784441747234No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14120.1231183470751545No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGGGGTGCCAGAGGTGACATCCAGATGACCCAGTCTCCCTCCTCC14010.12215920981040473No Hit
CTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGGTGCTA13830.12058971246808689No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13780.12015374098410972No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13570.11832266075140557No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT13410.11692755200267861No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13360.11649158051870144No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13130.11448611169240643No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12980.11317819724047488No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12900.11248064286611141No Hit
GTAATACATGGCCGTGTCCGCGGCGGTCACAGAGTTCACTTTCAGGGAGA12900.11248064286611141No Hit
GTCGTGTCCCTTGGCCGAAGGTGAGGGTAGTGTAACTCTGTTGACAGTAG12820.11178308849174794No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12740.11108553411738446No Hit
CACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACTACCCTCACCTTCGG12660.11038797974302096No Hit
AGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAAGTCCTGATCTATG12620.11003920255583924No Hit
ACCTTGATGGGACCCCACTTTGCAAATTGGATGCAGCATAGATCAGGACTTTAGGGGCTTTCCCTGGTTTCTG12610.10995200825904379No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12500.10899287099429401No Hit
CCATTAGCACCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCT12420.10829531661993051No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12390.10803373372954422No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12250.10681301357440812No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12230.10663862498081725No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12220.10655143068402181No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12220.10655143068402181No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12170.10611545920004464No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12160.10602826490324921No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12100.1055050991224766No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACT11950.10419718467054506No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATTTTG11780.10271488162502268No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11720.10219171584425006No Hit
GTTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACTTA11650.10158135576668202No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11580.10097099568911395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACG200.00777092551.041993
GTGGTAT2500.047.639191
CGCGTAT306.3386385E-445.95983539
GACAGGT2750.042.0709721
GCGTATG602.8911563E-740.28425240
GTATCAA36100.040.2491761
CTCTTAT2600.037.95431
CCCTAAG5300.036.68411628
GGGCGTA656.0722414E-736.645533
ACGAGAC5150.036.359622
ATCTCCG5500.035.8793710
TCTCCGA5500.035.8793711
CACGAGA5150.035.6907321
CGTATGC3600.035.5293841
CTTATAC5750.035.507471
TAAGACA5150.035.1894231
CCCACGA5400.034.6641919
TATCAAC42250.034.390422
TATACAC6150.033.7513433
ATCAACG43050.033.6723023
Produced by FastQC (version 0.11.5)