FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055663
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC82630.7827308525542716No Hit
GTATAATACTTATTCTCTCCTCTTCACTTTCGGCCCTGGGACCAGAGTGG67530.6396927807453705No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45980.4355556650180976No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGT39600.37511971149884005No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36260.3434808267411096No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34470.32652465796376307No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34030.32235666116933154No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC29680.28115032922438316No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28060.2658045228448852No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27280.2584158012547565No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27050.2562370756576673No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26630.2522585332629826No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23930.2266821892971526No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23800.2254507356987978No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23340.22109328450461938No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23330.2209985573047459No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23230.22005128530601148No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA23140.2191987405071505No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23100.2188198317076567No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22930.21720946930980814No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22710.21512547091259238No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA22660.21465183491322515No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA21320.20195839013018357No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA21060.19949548293347405No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20960.1985482109347396No Hit
GAGTGGATGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG20910.19807457493537234No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG20840.19741148453625823No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20480.19400130534081425No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20200.19134894374435782No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA20140.19078058054511715No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19360.18339185895498847No Hit
TACTTATTCTCTCCTCTTCACTTTCGGCCCTGGGACCAGAGTGGATGTCAAACGAACTGTGGCTGCACCAT19230.1821604053566337No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19120.1811184061580258No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18900.17903440776081003No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18890.1789396805609366No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT18760.17770822696258182No Hit
CCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAATACTCTGA18750.17761349976270835No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18280.17316132136865647No Hit
GTGCAGCCACAGTTCGTTTGACATCCACTCTGGTCCCAGGGCCGAAAGTGAAGAGGAGAGAATAAGTATTATA18170.17211932217004858No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17640.16709878057675603No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16900.16008896778612114No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16240.15383697259447382No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT16140.15288970059573936No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15510.14692188700371234No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15440.14625879660459826No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15400.14587988780510447No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15330.14521679740599036No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15260.14455370700687625No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15000.1420907998101667No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14940.141522436610926No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG14930.1414277094110526No Hit
GAATAAGTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTG14860.14076461901193846No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG14620.13849116621497579No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT14370.13612298621813967No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACATCCACTCTGGTCC14330.13574407741864591No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14310.13555462301889903No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC14300.13545989581902557No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14200.13451262382029114No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14150.1340389878209239No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14050.13309171582218945No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13750.13024989982598614No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13440.12731335662990936No Hit
GATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA13220.12522935823269357No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG13210.12513463103282013No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA13210.12513463103282013No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12690.12020881663940101No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12580.11916681744079313No Hit
CTCTGGTCCCAGGGCCGAAAGTGAAGAGGAGAGAATAAGTATTATACTGTTGGCAGTAATAAGTTGCAAAATCAT12290.11641972864446323No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12220.11575663824534912No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC11940.1131042766488927No Hit
GGACCAGAGTGGATGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11770.11149391425104413No Hit
GCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAACTAGA11760.11139918705117069No Hit
TTCTAAACTAGACGCCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT11710.11092555105180348No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG11690.11073609665205658No Hit
TTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC11610.10997827905306903No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT11510.10903100705433458No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAACT11220.10628391825800469No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11170.10581028225863746No Hit
ACTTATTACTGCCAACAGTATAATACTTATTCTCTCCTCTTCACTTTCGGCCCTGGGACCAGAGTGGATGTCAAA11170.10581028225863746No Hit
GGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCC11110.10524191905939678No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC11080.10495773745977646No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11080.10495773745977646No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG11080.10495773745977646No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10950.10372628386142169No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10950.10372628386142169No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10910.1033473750619279No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10910.1033473750619279No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10880.10306319346230758No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCAT10870.10296846626243414No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10820.10249483026306691No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10710.10145283106445901No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG10660.10097919506509179No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG10640.10078974066534489No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC10610.10050555906572457No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAATACTTATTCTCTCCTCTT10580.10022137746610424No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10560.10003192306635734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAA400.006729458552.92953570
GTTGTTG400.006729458552.92953570
CGTACTG305.382786E-447.50909863
GTGGTAT2450.047.4267921
GTATCAA20400.039.871051
TATCAAC22750.034.988132
ATCAACG23100.034.2971083
GGCTAAA400.002728357234.1525118
TCAACGC23800.033.2741784
CAACGCA23900.033.1349565
AACGCAG24250.032.9382446
CGCAGAG25300.031.436328
AGAGTAC25550.031.39592411
AAAACCG1352.5729338E-531.36565470
ACGCAGA25650.031.0073647
GTTCGCC450.00484703630.37074320
GCAGAGT26350.030.3131839
GACAGGT2350.029.0852661
CATGAGA3650.029.00248770
AATGGTA2952.1827873E-1128.70754670
Produced by FastQC (version 0.11.5)