FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275516
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTAAGCTGCTGACAGTAATAAGCTGCAAAATCTTCAGGCTGCAGGCTGCTGATGCTGAGAGTGAAATCTGTG42940.33664807027116866No Hit
GTGTATGCATCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAAAACTGGTAATG42800.3355504752586404No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40260.31563696574562766No Hit
GCTTAGTAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT35390.2774563392383945No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35250.27635874422586626No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28150.2206949971619329No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27890.2186566064243804No Hit
TGCATACACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACTCTCAGCAT27090.21238463492421888No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26650.20893505059913006No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25410.19921349477387976No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTACCAGTTTTTTAGCCTGGTATCAGCAAAAA24380.19113833146742182No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24030.18839434393610113No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT23900.1873751485673249No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG23690.18572875604853253No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC22060.17294961411695345No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21640.1696568290793687No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21370.16754003869806416No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT21130.1656584472480157No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20960.16432565330423138No Hit
GATTTCACTCTCAGCATCAGCAGCCTGCAGCCTGAAGATTTTGCAGCTTATTACTGTCAGCAGCTTAGTAGTTAC20130.15781848287281383No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20120.1577400832290618No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19990.15672088786028557No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19820.15538809391650124No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT19670.15421209926022095No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19090.14966491992260386No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19010.14903772277258773No Hit
GGGCATTACCAGTTTTTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGATGCATA18640.14613693595376304No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18160.14237375305366612No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC18010.14119775839738585No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17690.13868896979732123No Hit
GTAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGACATCAAACGA17420.13657217941601674No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17410.1364937797722647No Hit
GCTTATTACTGTCAGCAGCTTAGTAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGACATCAAACGAA17260.13531778511598444No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCGTCCTCC17180.13469058796596828No Hit
GTAATAAGCTGCAAAATCTTCAGGCTGCAGGCTGCTGATGCTGAGAGTGAAATCTGTGCCAGATCCACTGCCG16310.12786981895954266No Hit
CTCCTGTGCAGCCTCTGGATTCAGCTTTATCAGCTATGCCATGGCCTGGGTCCGCCAGGCTCCAGGGAAGGGG16300.12779141931579063No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCTCCGCCGAAAGTG15670.12285224175941345No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15250.11955945672182866No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15090.11830506242179635No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15050.11799146384678827No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14740.1155610748904757No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGG14680.11509067702796358No Hit
CAGTAATAAGCTGCAAAATCTTCAGGCTGCAGGCTGCTGATGCTGAGAGTGAAATCTGTGCCAGATCCACTGCCG14620.11462027916545148No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14550.11407148165918735No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGAGGGTAACTACTAAGCTGCTGACAGTAATAAGCTGCAAAATCTTCAGG14490.11360108379667522No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14380.11273868771540302No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14260.11179789199037879No Hit
GTACAAGATCTTTCGTACAGTAATATCGGGCCGTGTCGTCGGCTCTCAGG14150.11093549590910658No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14030.10999470018408238No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14000.1097595012528263No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13990.1096811016090743No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13950.10936750303406621No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13950.10936750303406621No Hit
ATGCATACACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGA13780.10803470909028189No Hit
ATATTACTGTACGAAAGATCTTGTACTGGGGGGTTTTGCAGTGGCTGGTGCGGACTACTGGGGCCAGGGAGCC13250.10387952797142491No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13250.10387952797142491No Hit
CAGTAATATCGGGCCGTGTCGTCGGCTCTCAGGTTGTTCATTTGCAGATA13080.10254673402764057No Hit
GGTAACTACTAAGCTGCTGACAGTAATAAGCTGCAAAATCTTCAGGCTGCAGGCTGCTGATGCTGAGAGTGAAAT13010.10199793652137645No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12960.10160593830261634No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12950.10152753865886434No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACTCTCAGCATCAGCAGCCTGCAGCCTGAAGATTTTG12950.10152753865886434No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)