FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275516
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52430.4110493321918345No Hit
CTACTAAGCTGCTGACAGTAATAAGCTGCAAAATCTTCAGGCTGCAGGCTGCTGATGCTGAGAGTGAAATCTGTG42350.3320224912897996No Hit
GTGTATGCATCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAAAACTGGTAATG41100.32222253582079724No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38660.30309302274530464No Hit
GCTTAGTAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT31320.24554768423132287No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27210.21332543064924314No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25930.20329027624898474No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25290.1982726990488555No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24540.19239272576745411No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23610.18510155889851637No Hit
TGCATACACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACTCTCAGCAT22780.1785943884670988No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTACCAGTTTTTTAGCCTGGTATCAGCAAAAA21010.16471765152299148No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20310.15922967646035016No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19910.1560936907102694No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT19330.15154651137265232No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19010.14903772277258773No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19000.1489593231288357No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18780.14723453096629127No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18580.1456665380912509No Hit
GATTTCACTCTCAGCATCAGCAGCCTGCAGCCTGAAGATTTTGCAGCTTATTACTGTCAGCAGCTTAGTAGTTAC17770.1393161669473374No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17610.1380617726473051No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17560.137669774428545No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17560.137669774428545No Hit
GAGTGAAATCTGTGCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA17310.13570978333474454No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT17130.1342985897472082No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17110.13414179045970415No Hit
GTAATAAGCTGCAAAATCTTCAGGCTGCAGGCTGCTGATGCTGAGAGTGAAATCTGTGCCAGATCCACTGCCG17090.1339849911722001No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16810.1317898011471436No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT16670.13069220613461532No Hit
CTCCTGTGCAGCCTCTGGATTCAGCTTTATCAGCTATGCCATGGCCTGGGTCCGCCAGGCTCCAGGGAAGGGG16590.13006500898459916No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16200.12700742287827044No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16170.12677222394701437No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC15790.12379303748443767No Hit
GCTTATTACTGTCAGCAGCTTAGTAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGACATCAAACGAA15760.12355783855318161No Hit
GGGCATTACCAGTTTTTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGATGCATA15580.12214664496564527No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCTCCGCCGAAAGTG15450.12112744959686904No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15400.12073545137810894No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15300.11995145494058876No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15060.1180698634905403No Hit
GTAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGACATCAAACGA14990.11752106598427617No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCGTCCTCC14840.11634507132799589No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14440.11320908557791515No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGAGGGTAACTACTAAGCTGCTGACAGTAATAAGCTGCAAAATCTTCAGG14420.11305228629041109No Hit
CAGTAATAAGCTGCAAAATCTTCAGGCTGCAGGCTGCTGATGCTGAGAGTGAAATCTGTGCCAGATCCACTGCCG14020.10991630054033034No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGG13790.10811310873403392No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13780.10803470909028189No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13770.10795630944652988No Hit
GTACAAGATCTTTCGTACAGTAATATCGGGCCGTGTCGTCGGCTCTCAGG13110.10278193295889662No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACT12980.10176273759012039No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12800.10035154400258406No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAA200.006617767353.15846658
GTATCAA29900.037.9696041
GACAGGT2400.035.4070361
CGACATT400.002794432533.9867521
TATCAAC35000.032.035852
ATCAACG35750.031.5402373
TCAACGC35850.031.4423944
CTCGAAA553.4258544E-431.0947733
CAACGCA36700.030.7117585
AACGCAG36900.030.6373026
TCCCTAG1753.7267855E-629.0442170
CGCAGAG39400.028.9518098
AGAGTAC39400.028.60265711
CAGAGTA40200.028.03674910
GCAGAGT40750.027.7427339
GATTCAC2800.026.7070221
ACAGGTA2550.026.6594161
GAGTTTA4250.024.7932571
ATTCACT2750.024.7108482
AATGGTA3251.4570105E-924.5758770
Produced by FastQC (version 0.11.5)