FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365393
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCTGCACTGGGGACATGAACAATATCGGCATCAACGGAGCGGCTTGGCTGCAGCAGCACCAGGGTCACCCTCC48170.35279219975494236No Hit
GTAATAGTCAGCCTCGTCCTCAGGTTGTAGTCCACTAATGGTCAGAGAGG44970.32935572395639934No Hit
GACTATTACTGCTCGTCATGGGACAACAGCCTCGATACTTGGGTGTTTGG42820.3136093417792533No Hit
GCCTTGGGCTGACCTAGGACGGTGAGCTTGGTCCCACCGCCAAACACCCA37300.2731814210267666No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC37040.27127720736813504No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC37020.27113072939439414No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG35030.2565561710071752No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29190.2137846026748343No Hit
ACCTAGGACGGTGAGCTTGGTCCCACCGCCAAACACCCAAGTATCGAGGC28020.20521564121099198No Hit
GACCTAGGACGGTGAGCTTGGTCCCACCGCCAAACACCCAAGTATCGAGG27350.20030862909067204No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTGAGCTTGGTCCCAC27290.1998691951694494No Hit
GTCTAAGGGCTTGAGACAGACCGCCACACTCACCTGCACTGGGGACATGAACAATATCGGCATCAACGGAGCGG26550.1944495101410363No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA26450.19371712027233184No Hit
CCCAAACTCCTGTCCTACAGGAATAATAACCGGCCCTCAGGAGTCTCAGA25270.1850749198216191No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24370.17848341100327889No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24200.17723834822648132No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23920.17518765659410881No Hit
CTATTACTGCTCGTCATGGGACAACAGCCTCGATACTTGGGTGTTTGGCG23510.1721848581324205No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23510.1721848581324205No Hit
GAATATCTGCATCCAGGTCAGGAAACACAGCCTCTCTGACCATTAGTGGA22180.16244407287865106No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21620.15834268961390602No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACGGCTC21530.15768353873207203No Hit
GTGCAGGTGAGTGTGGCGGTCTGTCTCAAGCCCTTAGACACCGAGGGTGGCTGAGTCAGTCCAGCCTGGAACA21500.15746382177146068No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21300.15599904203405174No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20920.15321596053297476No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20070.14699064664898678No Hit
ATACTTGGGTGTTTGGCGGTGGGACCAAGCTCACCGTCCTAGGTCAGCCC19580.1434019362923349No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19510.14288926338424177No Hit
CCCAAGTATCGAGGCTGTTGTCCCATGACGAGCAGTAATAGTCAGCCTCG19390.1420103955417964No Hit
CAGATATTCTGTCTGAGACTCCTGAGGGCCGGTTATTATTCCTGTAGGAC19010.13922731404071942No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18720.13710338342147646No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18610.13629775456590154No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18230.13351467306482456No Hit
TCATGGGACAACAGCCTCGATACTTGGGTGTTTGGCGGTGGGACCAAGCTCACCGTCCTAGGTCAGCCCAAGG17370.12721612019396614No Hit
TTAGTGGACTACAACCTGAGGACGAGGCTGACTATTACTGCTCGTCATGG17080.12509218957472318No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17020.12465275565350051No Hit
GTCTATTACTGTGCGAGAGAACCATATTTAGACAACTATGGTCTTGACTA17010.12457951666663004No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTGAGCTTGGTCCCACCGCCAAACAC16580.12143024023120083No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16540.12113728428371905No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16290.11930630961195787No Hit
GCATAGTGGGAATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTC16070.11769505190080803No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15700.1149852093866015No Hit
GTCTCAGACAGAATATCTGCATCCAGGTCAGGAAACACAGCCTCTCTGAC15480.1133739516754517No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15340.11234860585926543No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15300.11205564991178364No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15170.11110354308246782No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15120.1107373481481156No Hit
CAGTAATAGTCAGCCTCGTCCTCAGGTTGTAGTCCACTAATGGTCAGAGA14780.10824722259452041No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14580.10678244285711148No Hit
GTAATAGACGGCCGAGTCTTCAGATGTCAGGCTGTTCAACTCCATGTAGG14460.1059035750146661No Hit
GCACCAGGGTCACCCTCCCAAACTCCTGTCCTACAGGAATAATAACCGGCCCTCAGGAGTCTCAGACAGAATAT14330.1049514681853503No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14280.10458527325099808No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTGAGCTTGGTC14090.10319373250045959No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14030.1027542985792369No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14000.10253458161862555No Hit
CTCCTGTCCTACAGGAATAATAACCGGCCCTCAGGAGTCTCAGACAGAAT13880.1016557137761802No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13860.10150923580243928No Hit
GTCCCATGACGAGCAGTAATAGTCAGCCTCGTCCTCAGGTTGTAGTCCACTAATGGTCAGAGAGGCTGTGTTTC13840.10136275782869841No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)