FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004686074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365393
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCTGCACTGGGGACATGAACAATATCGGCATCAACGGAGCGGCTTGGCTGCAGCAGCACCAGGGTCACCCTCC45520.33338386823427396No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41370.3029896886830385No Hit
GTAATAGTCAGCCTCGTCCTCAGGTTGTAGTCCACTAATGGTCAGAGAGG40020.29310242545552817No Hit
GACTATTACTGCTCGTCATGGGACAACAGCCTCGATACTTGGGTGTTTGG38840.2844602250048155No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG37100.2717166412893577No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC33190.24308019742301298No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC32250.23619573265719102No Hit
GCCTTGGGCTGACCTAGGACGGTGAGCTTGGTCCCACCGCCAAACACCCA32210.23590277670970922No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26330.1928382524298865No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG25070.18361014008421017No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTGAGCTTGGTCCCAC24440.17899608391137203No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24280.1778242601214449No Hit
GTCTAAGGGCTTGAGACAGACCGCCACACTCACCTGCACTGGGGACATGAACAATATCGGCATCAACGGAGCGG24160.17694539227899952No Hit
ACCTAGGACGGTGAGCTTGGTCCCACCGCCAAACACCCAAGTATCGAGGC23310.17072007839501155No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22760.16669193411713698No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22600.1655201103272098No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTGAGCTTGGTCCCACCGCCAAACAC22380.16390885261605997No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACGGCTC21880.16024690327253766No Hit
GACCTAGGACGGTGAGCTTGGTCCCACCGCCAAACACCCAAGTATCGAGG21650.15856240657451737No Hit
GTGCAGGTGAGTGTGGCGGTCTGTCTCAAGCCCTTAGACACCGAGGGTGGCTGAGTCAGTCCAGCCTGGAACA21360.1564384759552744No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20950.15343567749358608No Hit
CTATTACTGCTCGTCATGGGACAACAGCCTCGATACTTGGGTGTTTGGCG20900.15306948255923386No Hit
CCCAAACTCCTGTCCTACAGGAATAATAACCGGCCCTCAGGAGTCTCAGA20630.1510920299137318No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20480.1499934451106751No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19780.14486671602974383No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19190.14054561580438746No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19160.1403258988437761No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18420.13490621381536305No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18000.13183017636680427No Hit
GAATATCTGCATCCAGGTCAGGAAACACAGCCTCTCTGACCATTAGTGGA17510.1282414660101524No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17130.12545838450907543No Hit
ATACTTGGGTGTTTGGCGGTGGGACCAAGCTCACCGTCCTAGGTCAGCCC16910.12384712679792559No Hit
CAGATATTCTGTCTGAGACTCCTGAGGGCCGGTTATTATTCCTGTAGGAC16880.12362740983731424No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16740.12260206402112798No Hit
CCCAAGTATCGAGGCTGTTGTCCCATGACGAGCAGTAATAGTCAGCCTCG16630.12179643516555307No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16550.1212105232705895No Hit
GTGGGAATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTCCTCAC15850.11608379418965821No Hit
GTAATAGACGGCCGAGTCTTCAGATGTCAGGCTGTTCAACTCCATGTAGGCCGTGTTCGCGGATGTGTCTCTGGT15660.11469225343911972No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15360.11249508383300631No Hit
GTCTATTACTGTGCGAGAGAACCATATTTAGACAACTATGGTCTTGACTA15350.11242184484613588No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14790.10832046158139085No Hit
TCATGGGACAACAGCCTCGATACTTGGGTGTTTGGCGGTGGGACCAAGCTCACCGTCCTAGGTCAGCCCAAGG14770.10817398360764996No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14760.10810074462077951No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC14620.10707539880459327No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14570.10670920387024102No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14390.105390902106573No Hit
GCATAGTGGGAATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTC14110.10334021047420047No Hit
GGTTGTAGTCCACTAATGGTCAGAGAGGCTGTGTTTCCTGACCTGGATGC14090.10319373250045959No Hit
TTAGTGGACTACAACCTGAGGACGAGGCTGACTATTACTGCTCGTCATGG13940.10209514769740288No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13900.10180219174992107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)