FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952483
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTATAGTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCACGTTGGTGATGGTGAGAGCGAAATCCGTG56700.5952862150820539No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG30930.3247302051585173No Hit
GTTATAGTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCACGTTGG27110.2846245024845588No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG26630.27958504246270016No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA23070.24220904730058174No Hit
GATCAGGACTGAGCAGAGAGAACTCACCATGGAGTTTGGGCTGAACTGGC18470.19391422209110293No Hit
CTATAACAGTTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT18300.19212941333336134No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17870.1876148970637796No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17570.18446523455011796No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC17470.18341534704556406No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15630.16409741696177255No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15470.16241759695448632No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC14960.1570631706812615No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACCTGTCGGGCGAGTCACG14880.1562232606776184No Hit
GGACTGAGCAGAGAGAACTCACCATGGAGTTTGGGCTGAACTGGCTTTTT14820.15559332817488605No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14360.1507638456539382No Hit
CTCCTGTGTAGCCTCTGGATTCATCTTTAGCAACTATGCCATGAGCTGGGTCCGCCAGGTTCCAGGGAAGGGC14010.1470892393879996No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT13140.13795521809838077No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC13070.13722029684519302No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12870.13512052183608528No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG12780.13417562308198677No Hit
CTCCAGGCCCTTCCCTGGAACCTGGCGGACCCAGCTCATGGCATAGTTGC12480.1310259605683251No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT12040.126406455548288No Hit
GTATCAACGCAGAGTACGGGTAACTGTTATAGTGTTGGCAGTAATAAGTT11920.12514659054282334No Hit
TCCTCACAGTCTGCATCTGTGGGAGACAGAGTCACCATCACCTGTCGGGC11900.12493661304191256No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11890.12483162429145717No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCTCAGGTGCCAGATGTGTCAT11780.1236767480364479No Hit
GTCTCCATCCTCACAGTCTGCATCTGTGGGAGACAGAGTCACCATCACCT11520.12094704052460778No Hit
CACTATAACAGTTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT11320.11884726551550003No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11280.11842731051367845No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT11210.11769238926049073No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC11130.11685247925684764No Hit
CCATAAATTAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC11050.11601256925320452No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT11040.11590758050274913No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCTCAGGTGCCAGATGTGTCATCCAG10940.11485769299819525No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG10930.11475270424773985No Hit
GTGAAATACGGCCGTGTCCTCGACTCTCAGACTGTTCATCTGCAAATACA10710.1124429517377213No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC10420.10939827797451503No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC10180.10687854796358572No Hit
GTTTCTCAGGTGCCAGATGTGTCATCCAGCTGACCCAGTCTCCATCCTCA10180.10687854796358572No Hit
CTCTCAGACTGTTCATCTGCAAATACAGGGTGTTCTTCGAATTGTCTCTG9970.10467378420402254No Hit
GGCCTGGAGTGGGTCTCATCTATTAGTGATAGTGGTTCCAGTACATATTA9920.10414884045174559No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT9920.10414884045174559No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT9910.1040438517012902No Hit
AATTTATGGTGCATCCAATCTGCAAAGTGGGGCCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACGGATTTCG9830.1032039416976471No Hit
GTGGGAGACAGAGTCACCATCACCTGTCGGGCGAGTCACGGCATTAATAATTATTTGGCCTGGTTTCAGCAGAAA9700.10183908794172704No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9680.10162911044081625No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG9600.10078920043717315No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAAG200.006649372653.09337654
ATCGTAT200.00745807751.57456239
GTTAACG200.00790110250.82726313
GTGGTAT2300.045.6852651
ACTAGAT1750.044.76703632
CGTACTA1750.044.64452429
ACCGTAC1750.044.5928927
GTACTAG1850.044.11388430
GGTATCA10250.043.9815031
AGACCGT1850.042.15144725
TGGTATC2650.040.930442
TACTAGA2000.040.83761631
TCGTATG1700.040.55000740
CGTATGC1800.040.2701541
GACCGTA1950.040.00454326
GGGTATC603.342666E-739.5447771
CTTATAC2150.039.41341
ATCTCGT1750.039.15931337
GCCGTCT2050.039.0672546
CTAGATC1750.038.9689933
Produced by FastQC (version 0.11.5)