FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952483
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTATAGTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCACGTTGGTGATGGTGAGAGCGAAATCCGTG60830.6386465690201295No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG32340.3395336189727271No Hit
GTTATAGTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCACGTTGG28090.29491340002918687No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA25880.27171088617854594No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23570.24745848482335117No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23200.24357390105650178No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC16450.17270649449911443No Hit
CTATAACAGTTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT16380.1719715732459267No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16130.16934685448454198No Hit
GATCAGGACTGAGCAGAGAGAACTCACCATGGAGTTTGGGCTGAACTGGC16010.16808698947907733No Hit
GGACTGAGCAGAGAGAACTCACCATGGAGTTTGGGCTGAACTGGCTTTTT14550.15275863191259056No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC14470.15191872190894745No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14010.1470892393879996No Hit
CTCCTGTGTAGCCTCTGGATTCATCTTTAGCAACTATGCCATGAGCTGGGTCCGCCAGGTTCCAGGGAAGGGC13920.14614434063390108No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACCTGTCGGGCGAGTCACG13890.1458293743825349No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC13220.13879512810202388No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC12730.13365067932970984No Hit
GTATCAACGCAGAGTACGGGTAACTGTTATAGTGTTGGCAGTAATAAGTT12550.13176088182151283No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12110.12714137680147572No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT12070.12672142179965415No Hit
GTGGGAGACAGAGTCACCATCACCTGTCGGGCGAGTCACGGCATTAATAATTATTTGGCCTGGTTTCAGCAGAAA11920.12514659054282334No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11830.12420169178872482No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11830.12420169178872482No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11650.12231189428052783No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC11180.11737742300912458No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCCGAGC10910.11454272674682908No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT10670.11202299673589976No Hit
CTCCAGGCCCTTCCCTGGAACCTGGCGGACCCAGCTCATGGCATAGTTGC10620.11149805298362281No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC10590.11118308673225664No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT10520.11044816547906891No Hit
CCATAAATTAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC10360.10876834547178271No Hit
GTGAAATACGGCCGTGTCCTCGACTCTCAGACTGTTCATCTGCAAATACA10290.10803342421859498No Hit
CACTATAACAGTTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT10150.10656358171221954No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA10070.10572367170857642No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC10030.10530371670675487No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCTCAGGTGCCAGATGTGTCAT9990.10488376170493333No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT9960.10456879545356715No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG9940.10435881795265638No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG9780.10267899794537015No Hit
GTTTCTCAGGTGCCAGATGTGTCATCCAGCTGACCCAGTCTCCATCCTCA9780.10267899794537015No Hit
GAGCAGAGAGAACTCACCATGGAGTTTGGGCTGAACTGGCTTTTTCTTGT9540.10015926793444084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGCA350.003608300861.95465570
CTGTATG400.006121705354.21032370
GTGGTAT2450.053.861351
TGGTATC2400.053.562372
CGCAATT200.00757642551.36959537
CGGAGTA200.00795290450.74332
GGTATCA8000.041.4490741
AATGGTA3850.041.303170
GTATCAA20750.037.9941371
TCCTTAT558.459541E-636.904222
TATCAAC23500.033.6849142
ACAGGTA1253.6379788E-1232.482541
CGTGCCA14150.032.18139370
ATCAACG24700.032.0315673
CAACGCA24700.031.8812855
TCAACGC24900.031.7642734
ACGGGTC652.6603328E-531.216816
AACGCAG25100.030.9692696
ATCCGTG22100.030.53549669
CTAAGGG450.005020779630.15249828
Produced by FastQC (version 0.11.5)