FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804579
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA71220.394662688638181No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAATCC70250.38928747369885164No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC63510.35193804205856327No Hit
CACCTGCACCTTGCGCAGTGGCGTCAATGTTGGTACCTACATGATATACTGGTACCAGCAGAAGCCAGGGAGTC63120.3497768731654308No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCGCTGTTGTGCCAAATCATACAGTAATAGTCAG57650.31946509407457363No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG54190.3002916469713989No Hit
GACTATTACTGTATGATTTGGCACAACAGCGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG54160.30012540321038866No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC47360.26244348404807993No Hit
GTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAG47070.2608364610249814No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46000.25490710021561813No Hit
CTCCAGAGCCCTGCTGCTTATCTGAGTCTGATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTG43680.24205091603083048No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG41460.22974887771607672No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA41180.22819726927998163No Hit
GTGCAGGTGAGACTGGCTGATGCTCCAGGAGATGCAGAGAGGGAAGACGG40490.2243736627767474No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGGG39890.22104878755654367No Hit
GTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA38400.21279201409303777No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT37560.20813718878475257No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG36830.2040919239335047No Hit
GGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCAC36340.20137660917033834No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT36240.2008224633003044No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG35730.19799631936313122No Hit
GGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCC35320.19572432129599202No Hit
TCCTCAGACTGGAGCCCAGAGATGAGTAAAATCCCTGCATTGGCCGAAGC35250.19533641918696826No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA34860.19317525029383584No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG34770.1926765190108053No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC34090.18890832709457442No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC34010.18846501039854724No Hit
GGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCT33640.18641467067942163No Hit
CCCCAGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTC33280.1844197455472994No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33260.1843089163732926No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAAT32230.1786012139119429No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA31560.1748884365827154No Hit
TCTCTGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATTTGG31180.17278268227658639No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG30940.17145273218850493No Hit
CTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAG30700.17012278210042342No Hit
CAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAA29780.16502464009611106No Hit
GTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGAAACA29210.16186600863691752No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC28750.15931693763476135No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA28320.15693411039361535No Hit
CTATTACTGTATGATTTGGCACAACAGCGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC28130.15588123324055084No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA28110.15577040406654405No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT28040.1553825019575203No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC27870.15444045397846257No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27600.1529442601293709No Hit
GTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCACAACAGCGCTTGGGTGTTCGGCGGAGGGACCAAG27360.15161431004128942No Hit
GTATATCATGTAGGTACCAACATTGACGCCACTGCGCAAGGTGCAGGTGA26800.14851109316909927No Hit
CCCAAGCGCTGTTGTGCCAAATCATACAGTAATAGTCAGCCTCATCCTCA26290.14568494923192613No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26290.14568494923192613No Hit
ATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATCATGTAGG26030.14424416996983785No Hit
CTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAG25970.14391168244781746No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25790.14291421988175634No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25610.14191675731569525No Hit
CCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCT25230.13981100300956623No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC24830.1375944195294304No Hit
GTATCAACGCAGAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTC24630.1364861277893625No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCGCTGTTGTGCCAAATCATACAGTAATAGTCAGC24450.13548866522330139No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT24250.1343803734832335No Hit
GAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA23870.13227461917710445No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACTCACTGTTTCTG23740.13155422954606033No Hit
GTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAG23210.12861725643488037No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC23130.12817393973885322No Hit
ATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAG22430.12429491864861554No Hit
GTGTATTACTGTGCGAGAGATCGCGTCGCCGATGCTTTTGATATCTGGGG22430.12429491864861554No Hit
GATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTGCTGGTA22240.12324204149555104No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG22080.12235540810349671No Hit
GTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGC21670.12008341003635752No Hit
GCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGT21650.11997258086235071No Hit
GCTTGGTCCCTCCGCCGAACACCCAAGCGCTGTTGTGCCAAATCATACAGTAATAGTCAGCCTCATCCTCAGA21530.11930760581830997No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC21300.11803307031723188No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG21010.11642604729413342No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC20660.11448653674901459No Hit
GTCTCACCTGCACCTTGCGCAGTGGCGTCAATGTTGGTACCTACATGATA20490.11354448876995688No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC20370.11287951372591612No Hit
GCTTATCTGAGTCTGATTTGTACCTCAGGAGATACTGGGGAGGACTCCCT20290.11243619702988895No Hit
CATGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCC20270.11232536785588218No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA20190.11188205115985501No Hit
ATGTTGGTACCTACATGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTACAAAT20130.11154956363783465No Hit
ATGCAGGGATTTTACTCATCTCTGGGCTCCAGTCTGAGGATGAGGCTGAC20110.11143873446382785No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20090.11132790528982106No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT20050.11110624694180748No Hit
GATTTTACTCATCTCTGGGCTCCAGTCTGAGGATGAGGCTGACTATTACT19890.11021961354975315No Hit
GTATATGGTAGTACTACTACCAGTAATGTATGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT19720.10927756557069543No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19360.10728264043857322No Hit
CTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT19350.10722722585156982No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19240.10661766539453246No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19060.10562020282847136No Hit
TCTTTGGATCCAGAGAAGCGGCTGGGGACTCCAGAGCCCTGCTGCTTATC18840.10440108191439666No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC18670.10345903393533892No Hit
CCAGTATATCATGTAGGTACCAACATTGACGCCACTGCGCAAGGTGCAGG18260.10118703586819973No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC18160.10063288999816578No Hit
GCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAATCCCTGCATTGGCCGAAGCATCTTTGGATCCAGAGAAG18100.1003004024761454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTA200.00747293751.55052638
ATATCGT200.00751350851.47997737
TCGTATG1650.048.0452840
GTGGTAT4200.045.4111941
CGTATGC2050.042.06983641
GGTATCA15600.041.9180261
ATCGTAT350.001365751739.3238839
GTATCAA37950.038.411051
TGGTATC5050.037.7666782
ACGAGAC3300.036.1175422
GTATGCC2400.035.97504442
TATACAC3750.035.4197543
AGGGGGG3500.034.70109670
TATCAAC43500.033.431112
ATGCCGT2300.033.14813644
TCTCCGA3600.033.1022411
ATCAACG44900.032.4645653
GATCTCG1800.032.38437736
TCAACGC45200.032.24824
CTTATAC3650.031.7256321
Produced by FastQC (version 0.11.5)