FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804579
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA65370.3622451552411947No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAATCC63010.34916731270839346No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC59940.33215503449835115No Hit
CACCTGCACCTTGCGCAGTGGCGTCAATGTTGGTACCTACATGATATACTGGTACCAGCAGAAGCCAGGGAGTC58950.326668990385015No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG56250.3117070518940983No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCGCTGTTGTGCCAAATCATACAGTAATAGTCAG52970.29353106735698464No Hit
GACTATTACTGTATGATTTGGCACAACAGCGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG49860.2762971307989287No Hit
GTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAG44590.24709364344813944No Hit
CTCCAGAGCCCTGCTGCTTATCTGAGTCTGATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTG43310.24000057631170485No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC42200.23384955715432795No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41720.23118965697816496No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40910.22670107543089No Hit
GTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA38340.21245952657101738No Hit
GTGCAGGTGAGACTGGCTGATGCTCCAGGAGATGCAGAGAGGGAAGACGG38300.2122378682230038No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG37350.20697348245768127No Hit
GGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCC36610.20287280301943003No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG36510.2023186571493961No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGGG36310.20121036540932816No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT36050.19976958614723989No Hit
GGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCAC36020.19960334238622973No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT34680.19217778772777475No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA34590.19167905644474417No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC34320.1901828625956525No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC34120.1890745708555846No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33350.18480764765632318No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG32870.1821477474801602No Hit
CCCCAGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTC32370.17937701812999043No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31970.17716043464985462No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA31290.17339224273362375No Hit
GGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCT31210.1729489260375966No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG30690.17006736751342003No Hit
TCCTCAGACTGGAGCCCAGAGATGAGTAAAATCCCTGCATTGGCCGAAGC29990.16618834642318236No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC29630.16419342129106013No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAAT29470.1633067878990058No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA29260.1621430815719345No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG28970.16053605854883604No Hit
TCTCTGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATTTGG28670.15887362093873417No Hit
GTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCACAACAGCGCTTGGGTGTTCGGCGGAGGGACCAAG27860.15438503939145917No Hit
CTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAG27150.15045060371421812No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA26810.14856650775610267No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26590.14734738684202797No Hit
GTATCAACGCAGAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTC25760.14274797612074616No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25640.14208300107670543No Hit
CTATTACTGTATGATTTGGCACAACAGCGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC25620.14197217190269862No Hit
GAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA25480.1411963676846511No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25380.14064222181461716No Hit
CCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCT25080.1389797842045153No Hit
GTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGAAACA24880.13787149246444738No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC24430.1353778360492946No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA24300.13465744641825045No Hit
CAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAA24170.13393705678720633No Hit
GTATATCATGTAGGTACCAACATTGACGCCACTGCGCAAGGTGCAGGTGA23880.13233003376410787No Hit
CTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAG23580.130667596154006No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC23370.1295038898269347No Hit
ATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATCATGTAGG23340.12933764606592452No Hit
CCCAAGCGCTGTTGTGCCAAATCATACAGTAATAGTCAGCCTCATCCTCA23190.1285064272608736No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC22880.12678857506376834No Hit
GTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAG22690.12573569791070383No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC22630.12540321038868343No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22540.1249044791056529No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT22470.12451657699662913No Hit
GTGTATTACTGTGCGAGAGATCGCGTCGCCGATGCTTTTGATATCTGGGG22160.12279872479952388No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCGCTGTTGTGCCAAATCATACAGTAATAGTCAGC22010.12196750599447294No Hit
ATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAG21860.12113628718942202No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21680.12013882462336091No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACTCACTGTTTCTG21450.1188642891222828No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20910.11587190142409948No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG20870.11565024307608589No Hit
GATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTGCTGGTA20850.11553941390207911No Hit
GCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGT20600.11415404922699422No Hit
GTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGC20540.11382156170497384No Hit
GCTTGGTCCCTCCGCCGAACACCCAAGCGCTGTTGTGCCAAATCATACAGTAATAGTCAGCCTCATCCTCAGA20110.11143873446382785No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19830.10988712602773279No Hit
ATGTTGGTACCTACATGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTACAAAT19680.10905590722268185No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC19330.10711639667756302No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC19040.10550937365446456No Hit
CTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT18960.10506605695843739No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT18890.10467815484941363No Hit
CTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT18890.10467815484941363No Hit
ATGCAGGGATTTTACTCATCTCTGGGCTCCAGTCTGAGGATGAGGCTGAC18750.10390235063136609No Hit
GTCTCACCTGCACCTTGCGCAGTGGCGTCAATGTTGGTACCTACATGATA18580.10296030265230836No Hit
GCTTATCTGAGTCTGATTTGTACCTCAGGAGATACTGGGGAGGACTCCCT18510.1025724005432846No Hit
GGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATCATGTAGGTACCA18210.10090996293318276No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18200.10085454834617937No Hit
GGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCT18180.10074371917217256No Hit
GTATATGGTAGTACTACTACCAGTAATGTATGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT18120.1004112316501522No Hit
GGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCA18060.10007874412813184No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAG150.00208075471.1938160
GTGGTAT5300.048.7943761
TGGTATC6050.041.604452
GGTATCA16900.040.4707641
ACGTCGG350.001439577938.90494526
GTATCAA37000.035.591091
GTCTCTT11900.032.72047470
TATCAAC42750.030.6328952
ATCAACG44200.029.616473
GACAGGT3350.029.4567811
TCAACGC44500.029.2558864
CAACGCA45600.028.6239015
AACGCAG45900.028.5841336
GGACTAT1005.701604E-727.2009963
CGCAGAG48850.026.7883878
ATTGTCT10100.026.76346264
TCGTCTC9550.026.71069520
ACGGGCT1156.4934284E-826.61188516
AGAGTAC50000.026.17080311
CTCGTCT9950.025.97728319
Produced by FastQC (version 0.11.5)