FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073037
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTAGCTCGCCGTACACTATTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT57410.5350234894043728No Hit
CTATTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA39870.3715622108091333No Hit
CTACTACACTGCTCACAGTAATACATTCCAAGATCTCCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC36790.3428586339520445No Hit
GTATTACTGTGAGCAGTGTAGTAGCTCGCCGTACACTATTGGCCAGGGGA36470.33987644414871065No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34300.31965346954485263No Hit
CAGTAATACATTCCAAGATCTCCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTCCCA31180.29057711896234706No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30610.28526509337515854No Hit
GTATTATTGTACAAGTGCCCCTCGCACATTCATTGCGCCTCCCTGGGACTACTGGGGCCAGGGAACCCTGGTCAC25570.2382956039726496No Hit
GGCCAATAGTGTACGGCGAGCTACTACACTGCTCACAGTAATACATTCCA25130.23419509299306548No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA22010.20511874241055994No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21610.20139100515639258No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21340.19887478250982957No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20600.19197846858961992No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19340.1802360962389927No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTCTGGGCTGAGCTGGCTCTTT18110.168773304182428No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17360.1617837968308642No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16830.1568445449690924No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16800.15656496467502984No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16730.15591261065555057No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16580.1545147091852378No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16490.15367596830305014No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15920.14836394271586162No Hit
GTCCCCAGGCACCCTGTCATTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCCGGGCCAGTCAGAGTGTTGCCAA15510.14454301203034006No Hit
CAATAATACAGGGCCGTGTCTTCGGGTCTCAGGCTGTTCATTTGCAGATA15480.1442634317362775No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15390.14342469085408985No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15350.1430519171286731No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAATCAAGTCATGACTTGGGTCCGCCAGGCTCCAGGGAAGGGG15210.14174720908971453No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14970.13951056673721407No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14630.13634199007117181No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14580.1358760229144009No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14340.13363938056190047No Hit
GTAGATGATGAGCCTGGGAGCCTGGCCAGGTCTTTGATGGTACCAGGCCAAGTAGTTGTTGGCAACACTCTGA14220.13252105938565026No Hit
CATCATCTACAGTGCATCCCACAGGCTCGATGGCATCCCAGACAGGTTCAGTGGGAGTGGGTCTGGGACAGACTT13760.1282341615433578No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13510.12590432575950317No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12890.12012633301554372No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12890.12012633301554372No Hit
CTTTATATCTGCAAATGAACAGCCTGAGACCCGAAGACACGGCCCTGTAT12840.11966036585877281No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12360.11518708115377195No Hit
GTCCCAGACCCACTCCCACTGAACCTGTCTGGGATGCCATCGAGCCTGTG12350.11509388772241777No Hit
GTCAGAGTGTTGCCAACAACTACTTGGCCTGGTACCATCAAAGACCTGGC12300.11462792056564684No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12010.11192531105637549No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGAC11680.10884992782168741No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11580.10791799350814556No Hit
GAGTGAAGTCTGTCCCAGACCCACTCCCACTGAACCTGTCTGGGATGCCA11560.1077316066454372No Hit
CTACTCAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAGACAATTTCAAGAACACTTTATATCTGCAAATGAA11530.10745202635137464No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11530.10745202635137464No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11340.10568135115564514No Hit
CCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGTAATCAAGTCATGA11280.10512219056752004No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT11160.10400386939126982No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11020.10269916135231125No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10980.10232638762689451No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)