FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073037
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTAGCTCGCCGTACACTATTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT51720.4819964269638419No Hit
CTACTACACTGCTCACAGTAATACATTCCAAGATCTCCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC34910.32533826885745787No Hit
CTATTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA34460.32114456444651956No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32420.3021331044502659No Hit
GTATTACTGTGAGCAGTGTAGTAGCTCGCCGTACACTATTGGCCAGGGGA31320.2918818270013056No Hit
CAGTAATACATTCCAAGATCTCCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTCCCA28680.26727876112380095No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26970.25134268436223545No Hit
GTATTATTGTACAAGTGCCCCTCGCACATTCATTGCGCCTCCCTGGGACTACTGGGGCCAGGGAACCCTGGTCAC23880.22254591407379243No Hit
GGCCAATAGTGTACGGCGAGCTACTACACTGCTCACAGTAATACATTCCA22390.20866009280201894No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21940.20446638839108064No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA21850.20362764750889295No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20700.19291040290316178No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19670.18331147947368076No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18360.1711031399662826No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17220.1604790887919056No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTCTGGGCTGAGCTGGCTCTTT15960.14873671644127837No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAATCAAGTCATGACTTGGGTCCGCCAGGCTCCAGGGAAGGGG15580.14519536604981934No Hit
GTCCCCAGGCACCCTGTCATTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCCGGGCCAGTCAGAGTGTTGCCAA15460.14407704487356912No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15070.14044250105075595No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14740.13736711781606786No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14520.1353168623262758No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14150.13186870536617096No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13880.129352482719608No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13780.12842054840606615No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13740.12804777468064943No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13660.12730222722981593No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13650.12720903379846174No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13400.12487919801460715No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13340.12432003742648204No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13340.12432003742648204No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12960.12077868703502302No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12870.11993994615283536No Hit
CAATAATACAGGGCCGTGTCTTCGGGTCTCAGGCTGTTCATTTGCAGATA12820.11947397899606445No Hit
GTAGATGATGAGCCTGGGAGCCTGGCCAGGTCTTTGATGGTACCAGGCCAAGTAGTTGTTGGCAACACTCTGA12630.11770330380033495No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11750.10950228184116671No Hit
CTTTATATCTGCAAATGAACAGCCTGAGACCCGAAGACACGGCCCTGTAT11700.10903631468439579No Hit
CATCATCTACAGTGCATCCCACAGGCTCGATGGCATCCCAGACAGGTTCAGTGGGAGTGGGTCTGGGACAGACTT11490.10707925262595792No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11340.10568135115564514No Hit
GAGTGAAGTCTGTCCCAGACCCACTCCCACTGAACCTGTCTGGGATGCCA11090.10335151537179055No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11020.10269916135231125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2800.051.19311
CGCGATA200.00769468651.1692218
GGTATCA11400.042.511231
TGGTATC3550.040.3607032
TGTAAGG350.001424330638.9878922
GTATCAA27250.038.3243751
CATCTGA18050.037.3254770
TATCAAC31400.033.0279582
AACGGCC851.246135E-732.35672437
ATCAACG32050.032.2321363
TAACGGC751.7255861E-632.0629736
TTAACGG751.7421098E-632.02385735
TCAACGC32500.031.887784
CAACGCA33000.031.6097665
AACGCAG33100.031.4098156
TATAGCT450.00490052230.3027114
AGAGTAC34850.029.63141611
ACGCAGA35250.029.4940387
CGCAGAG35250.029.3973378
GCAGAGT36000.028.879589
Produced by FastQC (version 0.11.5)