FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382568
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCACTATGGTGGCTCACCTCCGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA51640.37350784916184954No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT39470.2854832456703757No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36870.26667766070095644No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34060.24635316309939187No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGTCA32520.2352144704636589No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA32130.23239363271824603No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT26720.19326355014726218No Hit
GTGTATTACTGTCAGCACTATGGTGGCTCACCTCCGTGCAGTTTTGGCCA24570.17771277796101168No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24220.17518125690743602No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24030.17380700262120924No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23920.17301138171865688No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT23370.16903327720589512No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT22170.16035377645077856No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22090.1597751430671041No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21450.15514607599770863No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA21140.15290387163597016No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20820.1505893381012724No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20410.14762384200994091No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG19430.14053558305992905No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19230.13908899960074297No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCGTCCCCGACAGGTTCAGTGGCGGTGGGTCTGGGACAGACTTCA18950.13706378275788245No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18570.13431527418542885No Hit
GTCCCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGCGCCACCCTCTCCTGCGGGGCCAGTCAGAGTGTCAGCAG17900.12946921959715543No Hit
GTGTATTACTGTGCGAGCAGTTACTTTGATAGTAGGAACCACGCTTTTGA17800.1287459278675624No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17790.12867359869460307No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17650.12766099027317282No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17470.12635906515990533No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA17440.12614207764102742No Hit
CAGTTAGGACCAAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC17430.12606974846806812No Hit
GTCCCAGACCCACCGCCACTGAACCTGTCGGGGACGCCAGTGGCCCTGCT17320.12527412756551576No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT17120.12382754410632967No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA17050.12332123989561453No Hit
GTCTTTGTCTCCAGGGGAAAGCGCCACCCTCTCCTGCGGGGCCAGTCAGA16460.11905381869101557No Hit
GTTAGGACCAAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA16390.11854751448030043No Hit
CCATAGTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCT16070.11623298094560267No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC16030.11594366425376545No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15480.1119655597410037No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15330.11088062214661414No Hit
GGCCAAAACTGCACGGAGGTGAGCCACCATAGTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTG15240.11022965958998039No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGCTGGTACCAGGCTAAGAAGCTGCTGCTGACACTCTGA14920.10791512605528263No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14620.10574525086650349No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAG14480.10473264244507323No Hit
GAGGTGAGCCACCATAGTGCTGACAGTAATACACTGCAAAATCTTCAGGC14120.10212879221853825No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGT14030.10147782966190451No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13920.10068220875935217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15700.042.920441
CAACCGT359.714877E-442.1429666
GTATCAA40600.038.4490551
CGTATGC7050.036.75312841
GCCGTCT7250.036.52989646
TCGTATG7150.035.6992640
CTTATAC7600.035.644061
TATACAC7800.035.1642343
CCGTCTT7450.034.66921647
ATCTCGT6250.034.6059337
TATCAAC46250.033.7520332
ACATGCG7350.033.2429527
TCTCCGA7750.033.1992611
ATGCGCA7600.033.08804729
TTATACA8250.032.835742
CCCACGA8150.032.82552319
CATGCGC7650.032.40522428
CTTGAAA7950.032.27004657
TACACAT8450.032.058565
GTATGCC8100.032.0515342
Produced by FastQC (version 0.11.5)