FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382568
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66980.484460800481423No Hit
GTATTACTGTCAGCACTATGGTGGCTCACCTCCGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA46780.33835587110362747No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36020.2605296809994156No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT33360.24129012099224054No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA27050.19565041285491924No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGTCA25960.18776653300235502No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25740.18617529119725032No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT24560.17764044878805238No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23700.17142013991355218No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23140.1673697062278311No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22750.16454886848241823No Hit
GTGTATTACTGTCAGCACTATGGTGGCTCACCTCCGTGCAGTTTTGGCCA22280.1611493973533309No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT20650.14935974216096423No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20370.1473345253181037No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19300.1395953038114581No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19080.13800406200635337No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17510.12664838185174257No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCGTCCCCGACAGGTTCAGTGGCGGTGGGTCTGGGACAGACTTCA17350.12549111508439367No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA17180.1242615191440855No Hit
GTCTTTGTCTCCAGGGGAAAGCGCCACCCTCTCCTGCGGGGCCAGTCAGA17080.12353822741449245No Hit
GTGTATTACTGTGCGAGCAGTTACTTTGATAGTAGGAACCACGCTTTTGA16870.12201931478234705No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA16820.12165766891755053No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA16560.11977711042060861No Hit
GTCCCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGCGCCACCCTCTCCTGCGGGGCCAGTCAGAGTGTCAGCAG16500.11934313538285277No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16330.11811353944254459No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG16290.11782422275070739No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16280.11775189357774807No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15990.11565434756192823No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15610.11290583898947465No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15440.11167624304916647No Hit
CAGTTAGGACCAAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15240.11022965958998039No Hit
GTTAGGACCAAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15190.10986801372518387No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15170.10972335537926525No Hit
GTCCCAGACCCACCGCCACTGAACCTGTCGGGGACGCCAGTGGCCCTGCT14840.10733649267160819No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT14670.10610689673130001No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14430.10437099658027671No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14350.10379236319660227No Hit
CCATAGTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCT14060.10169481718078242No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13890.10046522124047425No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13850.10017590454863703No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCTA306.9462886E-445.11448322
CATACGT657.997418E-443.8386170
GGTATCA16850.040.158351
GTATCAA39550.036.2714731
GAGTTAT708.050829E-735.34929753
AATGGTA5250.033.92273370
TTCGGGG9900.032.3807970
TATCAAC44600.031.5461962
ATCAACG45400.031.0501963
TCAACGC45500.030.9696274
CAACGCA46350.030.3994835
AACGCAG46550.030.3414616
ACAGGTA3300.029.7323911
ACGCAGA48500.029.1215487
CGCAGAG48400.029.1119048
AGAGTAC49100.028.96899211
CCTTTGT13050.028.93180370
ATGGTCA10300.028.35677170
GACAGGC2700.027.5679661
GGCTAAC500.00850790327.0667271
Produced by FastQC (version 0.11.5)