FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences898223
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT46550.5182454691095641No Hit
CTGTTAGCCTGTTGACAATAGTAGATTGCAAAATCCTCAGGCTGCAGGCTATTGATGGTGAGAGTGAAATCTGTC22830.25416850826576476No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16680.185699987642267No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14610.16265448557874826No Hit
GTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA14300.1592032268156126No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12340.1373823649583678No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGATATTAGCAGCTGGTTAGTCTGGTATCAGCAGAAA12260.1364917175356231No Hit
GGATATTAGCAGCTGGTTAGTCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATGTATGCTGCATC11280.12558128660700071No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC10780.12001474021484643No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10310.1147821866062214No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10290.11455952475053523No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10220.11378020825563362No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG10030.111664920626615No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA9860.10977229485328253No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGT9810.10921564021406711No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC9620.10710035258504848No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9540.1062097051623038No Hit
CAATAGTAGATTGCAAAATCCTCAGGCTGCAGGCTATTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG9530.10609837423446071No Hit
CATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGACTAACC9410.10476240310034368No Hit
GTATCAACGCAGAGTACGGGAAACTGTTAGCCTGTTGACAATAGTAGATT9380.10442841031681444No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9370.10431707938897132No Hit
GTGTATTACTGTGCGAGAGATCCGGACATCTCTGGCAGTGGTTGGTTTTACTTTGACTTCTGGGGCCAGGGAACC9250.10298110825485432No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC9230.10275844639916815No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9110.10142247526505112No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG9080.10108848248152186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGTA1050.052.0778138
GGCGTAT1150.050.71581639
CTTATAC17900.046.9464071
TTATACA18150.046.1141932
GCGTATG1750.045.28030840
TATACAC18750.045.1699453
GTCGTAT2550.044.39827739
CGTATGC12800.043.7706841
ATCTCGT9350.043.72704337
ATGTCGT2500.043.61043537
CATCTCG9900.043.59723336
ATGCCGT12750.043.5222244
ACTCGAC18950.043.46290227
CCGTCTT12250.043.43110747
TCGACGT19050.043.1876629
GTATGCC13200.043.1283342
TCGTATG12150.043.10095640
GACTCGA19250.042.91559226
CGACGTC19150.042.88366730
TGCCGTC12750.042.8363845
Produced by FastQC (version 0.11.5)