FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1351527
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76170.5635847452548118No Hit
CATTATTACTGTGCAGCATGGGATGGCAGCCTGAATGGTTGGGTGTTCGG73780.5459010437823292No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC52070.38526792287538464No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46050.3407257124718929No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC44970.3327347511370472No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37530.27768590638588797No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACACCCAACCATTCAGGCTGCCATCCCATG37240.2755401852867164No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT34090.2522332147267498No Hit
GTAATAATGAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGC32880.24328037841641345No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32830.24291042650276318No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT32000.2367692247361688No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31720.23469749401972734No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG31640.2341055709578869No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30120.22285903278291885No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29370.2173097540781649No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC29270.2165698502508644No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG28890.2137582157071224No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28080.2077649947059881No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC27870.206211196668657No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC27400.20273364868034455No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC26580.19666643729648023No Hit
GCATGGGATGGCAGCCTGAATGGTTGGGTGTTCGGCGGAGGGACCGAGCTGACCGTCCTAGGTCAGCCCAAGG26080.19296691815997757No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG26050.1927449470117874No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25540.1889714374925547No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTC25040.18527191835605208No Hit
TTATTACTGTGCAGCATGGGATGGCAGCCTGAATGGTTGGGTGTTCGGCG24730.18297821649142046No Hit
GTCTGAGGATGAGGCTCATTATTACTGTGCAGCATGGGATGGCAGCCTGAATGGTTGGGTGTTCGGCGGAGGGAC23820.17624509166298566No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23780.17594913013206542No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23720.1755051878356851No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG23580.17446932247746438No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT23240.1719536494646426No Hit
CCCCTGAGGGCCGCTGATAATTAGTATAGATGACGAGTTTGGGGGCCGTTCCTGGGAGATGCTGGTACCAGTTTA22760.16840211109360007No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA22610.16729225535264927No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22470.16625638999442852No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21990.162704851623386No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21280.15745153444955223No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACAC20970.1551578325849206No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTATGTAAACTGGTACCAGCATCTCCCAGGAACGGCCCC20620.15256816918936877No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20520.15182826536206825No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC20450.15131033268295785No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20360.1506444192383874No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC20250.1498305250283568No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACCTCGGGCTCCCTGGCCATCAGTGGGCTCCAGT20180.14931259234924643No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19810.1465749481882345No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19570.14479917900271322No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19380.14339336173084224No Hit
GACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACACCCAACCATTCAGG19340.14309740019992204No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG19290.14272744828627176No Hit
GTCTGGCACCTCGGGCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG19180.14191355407624115No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19170.1418395636935111No Hit
GTGCCAGACTTGGAGGCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG18750.1387319676188489No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18260.1351064388650763No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA18220.1348104773341561No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTCATTATTACTGTGCAG17820.131850862024954No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT17320.12815134288845137No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC17270.1277813909748011No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC16920.12519172757924926No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC16760.12400788145556842No Hit
ATACTAATTATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACCTCGGGCTCCCTGG16680.12341595839372799No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC16480.12193615073912693No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16430.12156619882547667No Hit
CTGATGGCCAGGGAGCCCGAGGTGCCAGACTTGGAGGCAGAGAATCGGTC16430.12156619882547667No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16250.12023437193633571No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16020.1185325931335445No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTAT14990.11091158371234906No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC14800.10950576644047808No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14620.10817393955133711No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC14620.10817393955133711No Hit
GTAATACACAGCCGTGTCTTCACCTCTCAGGCTGATCATTTGGAGATACA14570.10780398763768685No Hit
GTGCAGCATGGGATGGCAGCCTGAATGGTTGGGTGTTCGGCGGAGGGACC14290.10573225692124537No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13930.10306860314296348No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA13840.102402689698393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGAC200.00634480553.7277661
ACCCGTT200.00787275450.874667
GGTATCA12900.041.3133131
GTGGTAT4200.038.7946661
TGGTATC4350.037.451372
GTATCAA31800.037.3612141
GTCACCG6050.032.6829670
TATCAAC36450.032.497032
ATCAACG38500.030.932673
GCCTAGT950.004498715530.83538670
TCAACGC38650.030.805784
CAACGCA39200.030.5432325
AACGCAG40100.030.1949396
AGAGTAC41500.029.17415211
ACGCAGA41900.028.897787
CGCAGAG42000.028.8289768
CAGAGTA42150.028.80364810
TCTGGAG8150.028.75446770
GACAGGT2500.028.514081
GCAGAGT42600.028.5014959
Produced by FastQC (version 0.11.5)